UniProt:Q96NY9 MUS81

chain
  • chain:1-551
checksum D0F331CC2269D847
comment
  • FUNCTION Catalytic subunit of two functionally distinct, structure-specific, heterodimeric DNA endonucleases MUS81-EME1 and MUS81-EME2 that are involved in the maintenance of genome stability (PubMed:11741546, PubMed:12374758, PubMed:12686547, PubMed:12721304, PubMed:24371268, PubMed:24733841, PubMed:24813886, PubMed:35290797, PubMed:39015284). Both endonucleases have essentially the same substrate specificity though MUS81-EME2 is more active than its MUS81-EME1 counterpart. Both cleave 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs (PubMed:24371268, PubMed:24733841, PubMed:35290797). MUS81-EME2 which is active during the replication of DNA is more specifically involved in replication fork processing (PubMed:24813886). Replication forks frequently encounter obstacles to their passage, including DNA base lesions, DNA interstrand cross-links, difficult-to-replicate sequences, transcription bubbles, or tightly bound proteins. One mechanism for the restart of a stalled replication fork involves nucleolytic cleavage mediated by the MUS81-EME2 endonuclease. By acting upon the stalled fork, MUS81-EME2 generates a DNA double-strand break (DSB) that can be repaired by homologous recombination, leading to the restoration of an active fork (PubMed:24813886). MUS81-EME2 could also function in telomere maintenance (PubMed:24813886). MUS81-EME1, on the other hand, is active later in the cell cycle and functions in the resolution of mitotic recombination intermediates including the Holliday junctions, the four-way DNA intermediates that form during homologous recombination (PubMed:11741546, PubMed:12374758, PubMed:14617801, PubMed:15805243, PubMed:24813886).COFACTOR Part of the heterodimeric DNA structure-specific endonuclease complex MUS81-EME1 (PubMed:12686547, PubMed:12721304, PubMed:14617801, PubMed:17289582, PubMed:35290797, PubMed:39015284). Part of the heterodimeric DNA structure-specific endonuclease complex MUS81-EME2 (PubMed:17289582, PubMed:24813886, PubMed:35290797). Interacts with BLM; may stimulate the endonuclease activity of MUS81 (PubMed:15805243). Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair (PubMed:19595721, PubMed:19596235, PubMed:19596236). Interacts with DCLRE1B/Apollo (PubMed:18469862). Interacts with RECQL5; this interaction stimulates mitotic DNA synthesis (PubMed:28575661). Interacts with CHEK2 (PubMed:11741546).INTERACTION Recruited to foci of DNA damage in S-phase cells.TISSUE SPECIFICITY Widely expressed.DEVELOPMENTAL STAGE Expressed in S phase and G2 phase.INDUCTION Up-regulated in cells treated with agents that damage DNA or block replication. This up-regulation seems to be independent of transcription.SIMILARITY Belongs to the XPF family.
created [InstanceEdit:143527] Schmidt, EE, 2004-11-12 07:45:10
crossReference
databaseName UniProt
dbId 150672
description
  • recommendedName: fullName evidence="25"Structure-specific endonuclease subunit MUS81 ecNumber evidence="21"3.1.22.- alternativeName: Crossover junction endonuclease MUS81 alternativeName: fullName evidence="26"MUS81 endonuclease homolog
displayName UniProt:Q96NY9 MUS81
geneName
  • MUS81
identifier Q96NY9
isSequenceChanged false
keyword
  • 3D-structure
  • DNA damage
  • DNA recombination
  • DNA repair
  • Endonuclease
  • Hydrolase
  • Magnesium
  • Metal-binding
  • Nuclease
  • Nucleus
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
modified [InstanceEdit:9963647] Weiser, Joel, 2025-08-15
moleculeType Protein
name
  • MUS81
otherIdentifier
  • 11757637_a_at
  • 16727111
  • 218463_PM_s_at
  • 218463_s_at
  • 3335644
  • 3335646
  • 3335647
  • 3335648
  • 3335649
  • 3335650
  • 3335651
  • 3335652
  • 3335653
  • 3335654
  • 3335655
  • 3335656
  • 3335657
  • 3335658
  • 3335659
  • 3335660
  • 3335661
  • 3335662
  • 3335663
  • 3335664
  • 3335665
  • 3335666
  • 3335667
  • 3335668
  • 3335669
  • 3335670
  • 3335671
  • 3335672
  • 3335673
  • 3335674
  • 3335675
  • 3335676
  • 3335677
  • 3335678
  • 3335679
  • 3335680
  • 3335681
  • 3335682
  • 3335683
  • 3335684
  • 3335685
  • 3335686
  • 3335687
  • 3335688
  • 3335690
  • 3335692
  • 3335694
  • 3335696
  • 56253_at
  • 7941425
  • 80198
  • 85124_at
  • A_14_P107000
  • A_14_P126179
  • A_23_P161628
  • A_23_P161634
  • A_24_P412238
  • GE62767
  • GO:0000712
  • GO:0000723
  • GO:0000727
  • GO:0000781
  • GO:0003677
  • GO:0003824
  • GO:0004518
  • GO:0004519
  • GO:0004520
  • GO:0005515
  • GO:0005634
  • GO:0005654
  • GO:0005657
  • GO:0005730
  • GO:0006281
  • GO:0006302
  • GO:0006308
  • GO:0006310
  • GO:0006974
  • GO:0008821
  • GO:0016787
  • GO:0016889
  • GO:0022414
  • GO:0031297
  • GO:0031573
  • GO:0032200
  • GO:0033687
  • GO:0043596
  • GO:0046872
  • GO:0048257
  • GO:0048476
  • GO:0071140
  • GO:0072429
  • GO:0140097
  • GO:1905347
  • HMNXSV003018455
  • HMNXSV003035803
  • ILMN_1780937
  • PH_hs_0014997
  • TC11000644.hg
  • g13376706_3p_s_at
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • MUS81_HUMAN
  • Q9H7D9
sequenceLength 551
species [Species:48887] Homo sapiens
stId uniprot:Q96NY9
url http://purl.uniprot.org/uniprot/Q96NY9
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