UniProt:Q8TDZ2 MICAL1

chain
  • chain:1-1067
checksum 78C1BBF3E3CCD56B
comment
  • FUNCTION Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:29343822). In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (PubMed:21864500, PubMed:26845023, PubMed:29343822). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels (By similarity). May act as Rab effector protein and play a role in vesicle trafficking. Promotes endosomal tubule extension by associating with RAB8 (RAB8A or RAB8B), RAB10 and GRAF (GRAF1/ARHGAP26 or GRAF2/ARHGAP10) on the endosomal membrane which may connect GRAFs to Rabs, thereby participating in neosynthesized Rab8-Rab10-Rab11-dependent protein export (PubMed:32344433).CATALYTIC ACTIVITY L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2OCATALYTIC ACTIVITY NADPH + O2 + H(+) = H2O2 + NADP(+)COFACTOR kcat is 0.28 sec(-1) for NADPH for the NADPH oxidase reaction.SUBUNIT Interacts with STK38 and STK38L (By similarity). Interacts with RAB1B, RAB8A, RAB10, RAB13, RAB15 and RAB35 (in their GTP-bound forms); binding to RAB1B is of low affinity compared to other Rab proteins; at least in case of RAB8A and RAB10 can bind 2 molecules of the Rab proteins simultaneously; ternary complex formation of RAB8A, RAB13 and MICAL1 is possible. Associates with the SH3 domain of NEDD9. Interacts with VIM and PLXNA3. Interacts with GRAF1/ARHGAP26, GRAF2/ARHGAP10, RAB8A, RAB8B and RAB10; may bind simultaneously to GRAFs and Rabs and connects GRAFs to Rabs (PubMed:32344433). Does not interact with RAB1 and RAB11A (PubMed:32344433).INTERACTION Accumulates transiently at the abscission site before abscission occurs. Colocalized with GRAF1/ARHGAP26 and GRAF2/ARHGAP10, RAB8A, RAB8B and RAB10 on endosomal tubules (PubMed:32344433).ALTERNATIVE PRODUCTS Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.DOMAIN The bivalent Mical/EHBP Rab binding (bMERB) domain, mediates binding to predominantly Rab8, Rab10, Rab13 and Rab15 (in their GTP-bound forms).DOMAIN The C-terminal coiled coil part contains the plexin-interacting region.SIMILARITY Belongs to the Mical family.CAUTION The reaction mechanism is subject to discussion. Some work suggest MICAL enzymes directly oxidize actin methionine residues to produce methionine-(R)-S-oxide. Other publications suggest that the enzyme functions as a NADPH oxidase producing H(2)O(2) (EC 1.6.3.1) and that it is the produced H(2)O(2) that is responsible for the methionine-(R)-S-oxide production.
crossReference
databaseName UniProt
dbId 59255
description
  • recommendedName: fullName evidence="28"[F-actin]-monooxygenase MICAL1 ecNumber evidence="22"1.14.13.225 ecNumber evidence="18 19 22"1.6.3.1 alternativeName: Molecule interacting with CasL protein 1 shortName: MICAL-1 alternativeName: NEDD9-interacting protein with calponin homology and LIM domains
displayName UniProt:Q8TDZ2 MICAL1
geneName
  • MICAL1
  • MICAL
  • NICAL
identifier Q8TDZ2
isSequenceChanged false
keyword
  • 3D-structure
  • Actin-binding
  • Alternative splicing
  • Coiled coil
  • Cytoplasm
  • Cytoskeleton
  • Endosome
  • FAD
  • Flavoprotein
  • LIM domain
  • Membrane
  • Metal-binding
  • Monooxygenase
  • NADP
  • Oxidoreductase
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • Zinc
modified [InstanceEdit:9948485] Weiser, Joel, 2025-05-21
moleculeType Protein
name
  • MICAL1
otherIdentifier
  • 11719686_a_at
  • 17022422
  • 218376_PM_s_at
  • 218376_s_at
  • 2968872
  • 2968874
  • 2968876
  • 2968878
  • 2968882
  • 2968884
  • 2968886
  • 2968890
  • 2968892
  • 2968894
  • 2968898
  • 2968900
  • 2968902
  • 2968904
  • 2968906
  • 2968908
  • 2968910
  • 2968912
  • 2968914
  • 2968916
  • 2968918
  • 2968920
  • 2968922
  • 2968924
  • 2968926
  • 2968928
  • 2968930
  • 2968932
  • 2968934
  • 2968936
  • 2968938
  • 2968940
  • 2968942
  • 2968944
  • 2968946
  • 2968948
  • 2968950
  • 2968952
  • 2968954
  • 2968956
  • 2968958
  • 2968960
  • 2968962
  • 2968964
  • 2968966
  • 2968968
  • 2968970
  • 2968972
  • 2968974
  • 2968978
  • 2968980
  • 2968984
  • 2968986
  • 2968988
  • 2968990
  • 2968992
  • 2968994
  • 2968996
  • 2968998
  • 2969000
  • 2969002
  • 2969024
  • 2969026
  • 2969028
  • 2969030
  • 2969032
  • 44693_at
  • 64780
  • 8128737
  • A_24_P283341
  • A_33_P3324884
  • GE559908
  • GE56564
  • GO:0003779
  • GO:0004497
  • GO:0005515
  • GO:0005654
  • GO:0005737
  • GO:0005768
  • GO:0005815
  • GO:0005829
  • GO:0005856
  • GO:0005882
  • GO:0005884
  • GO:0005886
  • GO:0005929
  • GO:0006790
  • GO:0007010
  • GO:0007165
  • GO:0008092
  • GO:0010008
  • GO:0015629
  • GO:0016020
  • GO:0016174
  • GO:0016491
  • GO:0016709
  • GO:0017124
  • GO:0019417
  • GO:0019901
  • GO:0030042
  • GO:0030496
  • GO:0031267
  • GO:0036064
  • GO:0043066
  • GO:0043226
  • GO:0045171
  • GO:0046872
  • GO:0051015
  • GO:0051017
  • GO:0071949
  • GO:0120025
  • GO:0120501
  • GO:1903305
  • GO:1990026
  • ILMN_1807972
  • PH_hs_0002801
  • TC06002003.hg
  • g12232438_3p_s_at
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • MICA1_HUMAN
  • B7Z3R5
  • E1P5F0
  • Q7Z633
  • Q8IVS9
  • Q96G47
  • Q9H6X6
  • Q9H7I0
  • Q9HAA1
  • Q9UFF7
sequenceLength 1067
species [Species:48887] Homo sapiens
stId uniprot:Q8TDZ2
url http://purl.uniprot.org/uniprot/Q8TDZ2
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