Reactome | UniProt:Q8TDX7 NEK7

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UniProt:Q8TDX7 NEK7 Show undefined attributes

chain	chain:1-302
checksum	C1FC990B34D94D4C
comment	FUNCTION Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132, PubMed:19941817, PubMed:31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596, PubMed:19941817, PubMed:26522158, PubMed:31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed:26642356, PubMed:36442502, PubMed:39173637). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 (PubMed:26642356, PubMed:31189953, PubMed:36442502, PubMed:39173637, PubMed:40450990). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed:26642356, PubMed:31189953).CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)COFACTOR Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97.SUBUNIT Monomer (PubMed:21320329). Interacts with NEK9; interaction takes place during mitosis; it relieves NEK7 autoinhibition and prevents interaction with NLRP3 (PubMed:19414596, PubMed:19941817, PubMed:26522158). Interacts with ANKS3; this interaction alters the subcellular distribution of NEK7 by preventing its nuclear translocation (PubMed:26188091). Interacts (via N-terminus) with NLRP3 (via LRR repeat domain); the interaction is required for the formation of the complex NLRP3:PYCARD, oligomerization of PYCARD and activation of CASP1 (PubMed:31189953, PubMed:36442502).SUBUNIT (Microbial infection) Interacts with human cytomegalovirus protein US18; this interaction promotes inflammasome assembly.INTERACTION Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity).ALTERNATIVE PRODUCTS Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.DOMAIN Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.DOMAIN The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.PTM Phosphorylation at Ser-195 required for its activation.SIMILARITY Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
crossReference	[DatabaseIdentifier:12219795] PDB:8WS0 [DatabaseIdentifier:12817971] ZINC - World Drugs:NEK7_HUMAN [DatabaseIdentifier:12815237] ZINC target:Q8TDX7 [DatabaseIdentifier:12811891] ZINC - Substances:NEK7_HUMAN [DatabaseIdentifier:12809856] ZINC - Predictions - Purchasable:NEK7_HUMAN [DatabaseIdentifier:12806482] ZINC - Investigational:NEK7_HUMAN [DatabaseIdentifier:12805426] ZINC - FDA approved:NEK7_HUMAN [DatabaseIdentifier:12804247] ZINC - Biogenic:NEK7_HUMAN [DatabaseIdentifier:12340972] RefSeq:XP_047302520.1 [DatabaseIdentifier:12340967] RefSeq:XP_047302519.1 [DatabaseIdentifier:12340970] RefSeq:XP_054190475.1 [DatabaseIdentifier:12340968] RefSeq:XP_054190474.1 [DatabaseIdentifier:12340969] RefSeq:XP_047302518.1 [DatabaseIdentifier:12340971] RefSeq:XP_054190473.1 [DatabaseIdentifier:12340973] RefSeq:NP_598001.1 [DatabaseIdentifier:12291721] Pharos - Targets:Q8TDX7 [DatabaseIdentifier:12253783] PRO:Q8TDX7 [DatabaseIdentifier:12150457] PDB:8EJ4 [DatabaseIdentifier:12150453] PDB:8SXN [DatabaseIdentifier:12219798] PDB:2WQN [DatabaseIdentifier:12219797] PDB:2WQM [DatabaseIdentifier:11460369] ENSEMBL:ENSG00000151414 [DatabaseIdentifier:11553149] ENSEMBL:ENSP00000356355 [DatabaseIdentifier:11553148] ENSEMBL:ENSP00000356353 [DatabaseIdentifier:11553150] ENSEMBL:ENSP00000444621 [DatabaseIdentifier:11675847] ENSEMBL:ENST00000538004 [DatabaseIdentifier:11675848] ENSEMBL:ENST00000367383 [DatabaseIdentifier:11675849] ENSEMBL:ENST00000367385 [DatabaseIdentifier:11780300] Guide to Pharmacology - Targets:2122 [DatabaseIdentifier:11793879] GeneCards:NEK7 [DatabaseIdentifier:11809841] GlyGen:Q8TDX7 [DatabaseIdentifier:11830744] HMDB Protein:HMDBP01432 [DatabaseIdentifier:11846868] HPA:ENSG00000151414-NEK7 [DatabaseIdentifier:12131530] OpenTargets:ENSG00000151414 [DatabaseIdentifier:12144457] PDB:5DE2 [DatabaseIdentifier:12219792] PDB:6S73 [DatabaseIdentifier:12219794] PDB:6S76 [DatabaseIdentifier:12219793] PDB:6S75 [DatabaseIdentifier:12150465] PDB:6NPY [DatabaseIdentifier:12219796] PDB:8WS1
databaseName	UniProt
dbId	96135
description	recommendedName: fullName evidence="27"Serine/threonine-protein kinase Nek7 ecNumber evidence="13 16 19"2.7.11.34 alternativeName: fullName evidence="23"Never in mitosis A-related kinase 7 shortName evidence="23"NimA-related protein kinase 7
displayName	UniProt:Q8TDX7 NEK7
geneName	NEK7
identifier	Q8TDX7
isSequenceChanged	false
keyword	3D-structure Acetylation Alternative splicing ATP-binding Cytoplasm Cytoskeleton Kinase Magnesium Metal-binding Microtubule Nucleotide-binding Nucleus Phosphoprotein Proteomics identification Reference proteome Serine/threonine-protein kinase Transferase
modified	[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType	Protein
name	NEK7
otherIdentifier	11717450_a_at 11717451_at 11717452_x_at 140609 16675558 212530_PM_at 212530_at 2373749 2373750 2373751 2373766 2373771 2373773 2373774 2373776 2373781 2373782 2373785 2373786 2373789 2373790 2373796 2373798 2373802 2373804 2373805 2373815 2373816 2373817 2373818 2373823 2373825 2373827 2373829 2373831 34752_at 69905_r_at 7908543 91600_at A_14_P103773 A_14_P118223 A_23_P201619 A_23_P353574 A_33_P3318475 GE471590 GE56338 GO:0000166 GO:0000922 GO:0003824 GO:0004672 GO:0004674 GO:0005515 GO:0005524 GO:0005634 GO:0005654 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005874 GO:0006468 GO:0006954 GO:0007010 GO:0007346 GO:0009306 GO:0016301 GO:0016740 GO:0032206 GO:0032731 GO:0032741 GO:0033044 GO:0035655 GO:0035865 GO:0044546 GO:0046872 GO:0050729 GO:0051225 GO:0051604 GO:0065003 GO:0070269 GO:0070498 GO:0098772 GO:0106310 GO:0140096 GO:0140677 GO:1900227 HMNXSV003037969 HMNXSV003046155 Hs.24119.0.S1_3p_at ILMN_1758548 ILMN_1869897 PH_hs_0027102 PH_hs_0040147 TC01001639.hg
physicalEntity	[EntityWithAccessionedSequence:R-HSA-2530599] NEK7 [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-2984246] p-S195-NEK7 [cytosol] - Homo sapiens
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:ensembl:ENSG00000151414] ENSEMBL:ENSG00000151414 NEK7 [ReferenceDNASequence:12678437] UCSC:Q8TDX7 NEK7 [ReferenceDNASequence:11324973] BioGPS Gene:140609 NEK7 [ReferenceDNASequence:11391888] COSMIC - genes:NEK7 NEK7 [ReferenceDNASequence:11422495] CTD Gene:140609 NEK7 [ReferenceDNASequence:11427605] dbSNP Gene:140609 NEK7 [ReferenceDNASequence:11825574] HGNC:13386 NEK7 [ReferenceDNASequence:11867416] KEGG:hsa:140609 NEK7 [ReferenceDNASequence:11954762] Monarch:140609 NEK7 [ReferenceDNASequence:12038855] NCBI Gene:140609 NEK7 [ReferenceDNASequence:12115984] OMIM:606848 NEK7
referenceTranscript	[ReferenceRNASequence:12522872] RefSeq:XM_047446564.1 NEK7 [ReferenceRNASequence:12522870] RefSeq:XM_054334499.1 NEK7 [ReferenceRNASequence:12522875] RefSeq:XM_054334500.1 NEK7 [ReferenceRNASequence:12522869] RefSeq:NM_133494.3 NEK7 [ReferenceRNASequence:12522874] RefSeq:XM_054334498.1 NEK7 [ReferenceRNASequence:12522873] RefSeq:XM_047446562.1 NEK7 [ReferenceRNASequence:12522871] RefSeq:XM_047446563.1 NEK7
schemaClass	ReferenceGeneProduct
secondaryIdentifier	NEK7_HUMAN A6NGT8
sequenceLength	302
species	[Species:48887] Homo sapiens
stId	uniprot:Q8TDX7
url	http://purl.uniprot.org/uniprot/Q8TDX7

Referrals

(referenceEntity)	[EntityWithAccessionedSequence:R-HSA-2530599] NEK7 [cytosol] [EntityWithAccessionedSequence:R-HSA-2984246] p-S195-NEK7 [cytosol]
(referenceSequence)	[ModifiedResidue:2984252] O-phospho-L-serine at 195
(interactor)	[UndirectedInteraction:11184471] UniProt:P16422 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11184595] UniProt:P07237 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11196061] UniProt:Q9ULW0 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11207807] UniProt:P26715 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11210221] UniProt:P07437 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11217317] UniProt:P61224 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11230955] UniProt:Q10981 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11234731] UniProt:P00558 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11236081] UniProt:Q8N2W9 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11245301] UniProt:Q96P20 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11269417] UniProt:Q13200 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11271079] UniProt:P43686 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11283391] UniProt:Q9HC35 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11290835] UniProt:P52756 <-> UniProt:Q8TDX7 (IntAct) [UndirectedInteraction:11297331] UniProt:Q7Z434 <-> UniProt:Q8TDX7 (IntAct)