Reactome | UniProt:Q8TD19 NEK9

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UniProt:Q8TD19 NEK9 Show undefined attributes

chain	initiator methionine:1 chain:2-979
checksum	8583FDDE599324A2
comment	FUNCTION Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation (PubMed:12101123, PubMed:12840024, PubMed:14660563, PubMed:19941817). Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2 (PubMed:11864968). Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues (PubMed:11864968). Important for G1/S transition and S phase progression (PubMed:12840024, PubMed:14660563, PubMed:19941817). Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively (PubMed:12840024, PubMed:14660563, PubMed:19941817, PubMed:26522158).CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)COFACTOR Activated during mitosis by intramolecular autophosphorylation (PubMed:11864968). Activity and autophosphorylation is activated by manganese >> magnesium ions (PubMed:11864968). Sensitive to increasing concentration of detergents (PubMed:11864968). It is not cell-cycle regulated but activity is higher in G0-arrested cells (PubMed:11864968).SUBUNIT Homodimer; homodimerization is required to activate NEK7 (PubMed:23482567, PubMed:26522158). Binds to Ran GTPase (PubMed:12101123). Has a greater affinity for Ran-GDP over Ran-GTP (PubMed:12101123). Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex (PubMed:14660563). Interacts with DYNLL1; phosphorylation at Ser-944 strongly reduces DYNLL1 binding (PubMed:23482567).INTERACTION Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.DEVELOPMENTAL STAGE Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.DOMAIN Dimerizes through its coiled-coil domain.PTM Autophosphorylated on serine and threonine residues (PubMed:27153399). When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro (PubMed:14660563).DISEASE The disease is caused by variants affecting the gene represented in this entry.DISEASE The disease is caused by variants affecting the gene represented in this entry.DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.SEQUENCE CAUTION Extended N-terminus.
crossReference	[DatabaseIdentifier:12810562] ZINC - Predictions - Purchasable:NEK9_HUMAN [DatabaseIdentifier:12818602] ZINC - World Drugs:NEK9_HUMAN [DatabaseIdentifier:12815701] ZINC target:Q8TD19 [DatabaseIdentifier:12812335] ZINC - Substances:NEK9_HUMAN [DatabaseIdentifier:11461140] ENSEMBL:ENSG00000119638 [DatabaseIdentifier:11554317] ENSEMBL:ENSP00000238616 [DatabaseIdentifier:11677016] ENSEMBL:ENST00000238616 [DatabaseIdentifier:11781011] Guide to Pharmacology - Targets:2124 [DatabaseIdentifier:11794428] GeneCards:NEK9 [DatabaseIdentifier:11810372] GlyGen:Q8TD19 [DatabaseIdentifier:11831459] HMDB Protein:HMDBP01300 [DatabaseIdentifier:11847402] HPA:ENSG00000119638-NEK9 [DatabaseIdentifier:11851030] IntEnz:2.7.11.38 [DatabaseIdentifier:12132077] OpenTargets:ENSG00000119638 [DatabaseIdentifier:12136317] Orphanet:NEK9 [DatabaseIdentifier:12167739] PDB:3ZKF [DatabaseIdentifier:12167738] PDB:3ZKE [DatabaseIdentifier:12254499] PRO:Q8TD19 [DatabaseIdentifier:12292261] Pharos - Targets:Q8TD19 [DatabaseIdentifier:12342730] RefSeq:NP_149107.4 [DatabaseIdentifier:12342728] RefSeq:NP_001316166.1 [DatabaseIdentifier:12342729] RefSeq:NP_001316167.1 [DatabaseIdentifier:12804884] ZINC - Biogenic:NEK9_HUMAN [DatabaseIdentifier:12805955] ZINC - FDA approved:NEK9_HUMAN [DatabaseIdentifier:12807047] ZINC - Investigational:NEK9_HUMAN
databaseName	UniProt
dbId	96139
description	recommendedName: fullName evidence="21"Serine/threonine-protein kinase Nek9 ecNumber evidence="5 7"2.7.11.38 alternativeName: fullName evidence="19"Nercc1 kinase alternativeName: fullName evidence="20"Never in mitosis A-related kinase 9 shortName evidence="20"NimA-related protein kinase 9 alternativeName: fullName evidence="18"NimA-related kinase 8 shortName evidence="18"Nek8
displayName	UniProt:Q8TD19 NEK9
geneName	NEK9 KIAA1995 NEK8 NERCC
identifier	Q8TD19
isSequenceChanged	false
keyword	3D-structure Acetylation ATP-binding Cell cycle Cell division Coiled coil Cytoplasm Direct protein sequencing Disease variant Kinase Magnesium Metal-binding Mitosis Nucleotide-binding Nucleus Phosphoprotein Proteomics identification Reference proteome Repeat Serine/threonine-protein kinase Transferase
modified	[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType	Protein
name	NEK9
otherIdentifier	11736077_a_at 11736078_a_at 11736079_a_at 16794898 212299_PM_at 212299_at 214738_3p_s_at 214738_PM_s_at 214738_s_at 3572143 3572144 3572279 3572280 3572281 3572282 3572283 3572284 3572285 3572287 3572288 3572289 3572291 3572292 3572293 3572295 3572296 3572297 3572298 3572299 3572300 3572302 3572303 3572304 3572305 3572306 3572307 3572308 3572309 3572310 3572312 3572313 3572314 3572315 3572316 3572318 3572320 3572321 3572322 3572323 3572337 3572339 35843_at 60722_at 7980271 91754 A_23_P3131 A_24_P347480 GE501409 GE56489 GE890268 GE905911 GO:0000166 GO:0000278 GO:0003824 GO:0004672 GO:0004674 GO:0005515 GO:0005524 GO:0005634 GO:0005737 GO:0005813 GO:0005815 GO:0005829 GO:0006468 GO:0007346 GO:0016301 GO:0016740 GO:0019901 GO:0030295 GO:0046872 GO:0051301 GO:0098772 GO:0106310 GO:0140096 GO:1900227 HMNXSV003034185 Hs.306533.0.S1_3p_s_at Hs.7200.0.A1_3p_at ILMN_1847889 PH_hs_0000740 TC14001317.hg
physicalEntity	[EntityWithAccessionedSequence:R-HSA-2530600] p-T333-NEK9 [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-2984213] p-S29,T333,S750,S869-NEK9 [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-2984225] p-S29,T210,T333,S750,S869-NEK9 [cytosol] - Homo sapiens
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:ensembl:ENSG00000119638] ENSEMBL:ENSG00000119638 NEK9 [ReferenceDNASequence:11325611] BioGPS Gene:91754 NEK9 [ReferenceDNASequence:11392018] COSMIC - genes:NEK9 NEK9 [ReferenceDNASequence:11392019] COSMIC - genes:NEK8 NEK9 [ReferenceDNASequence:11423179] CTD Gene:91754 NEK9 [ReferenceDNASequence:11427757] dbSNP Gene:91754 NEK9 [ReferenceDNASequence:11826125] HGNC:18591 NEK9 [ReferenceDNASequence:11867989] KEGG:hsa:91754 NEK9 [ReferenceDNASequence:11955400] Monarch:91754 NEK9 [ReferenceDNASequence:12039493] NCBI Gene:91754 NEK9 [ReferenceDNASequence:12116471] OMIM:609798 NEK9 [ReferenceDNASequence:12678859] UCSC:Q8TD19 NEK9
referenceTranscript	[ReferenceRNASequence:12524610] RefSeq:NM_033116.5 NEK9 [ReferenceRNASequence:12524608] RefSeq:NM_001329238.1 NEK9 [ReferenceRNASequence:12524609] RefSeq:NM_001329237.1 NEK9
schemaClass	ReferenceGeneProduct
secondaryIdentifier	NEK9_HUMAN Q52LK6 Q8NCN0 Q8TCY4 Q9UPI4 Q9Y6S4 Q9Y6S5 Q9Y6S6
sequenceLength	979
species	[Species:48887] Homo sapiens
stId	uniprot:Q8TD19
url	http://purl.uniprot.org/uniprot/Q8TD19

Referrals

(referenceEntity)	[EntityWithAccessionedSequence:R-HSA-2530600] p-T333-NEK9 [cytosol] [EntityWithAccessionedSequence:R-HSA-2984213] p-S29,T333,S750,S869-NEK9 [cytosol] [EntityWithAccessionedSequence:R-HSA-2984225] p-S29,T210,T333,S750,S869-NEK9 [cytosol]
(referenceSequence)	[ModifiedResidue:2984209] O-phospho-L-threonine at 333 [ModifiedResidue:2984207] O-phospho-L-serine at 29 [ModifiedResidue:2984223] O-phospho-L-serine at 750 [ModifiedResidue:2984211] O-phospho-L-serine at 869 [ModifiedResidue:2984228] O-phospho-L-threonine at 210
(interactor)	[UndirectedInteraction:11203653] UniProt:Q9GZQ8 <-> UniProt:Q8TD19 (IntAct) [UndirectedInteraction:11279447] UniProt:P08238 <-> UniProt:Q8TD19 (IntAct) [UndirectedInteraction:11285945] UniProt:P53350 <-> UniProt:Q8TD19 (IntAct)