UniProt:Q8QGX4 PRKDC

chain
  • chain:1-4134
checksum 8907B1EA6E7E9AA8
comment
  • FUNCTION Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). Recruited by XRCC5 and XRCC6 to DNA ends and is required to (1) protect and align broken ends of DNA, thereby preventing their degradation, (2) and sequester the DSB for repair by NHEJ. Acts as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome (By similarity). Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX, thereby regulating DNA damage response mechanism (By similarity).CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)SUBUNIT DNA-PK is a heterotrimer of PRKDC and the Ku dimer (composed of XRCC6/Ku70 and XRCC5/Ku86). Component of the core long-range non-homologous end joining (NHEJ) complex (also named DNA-PK complex) composed of PRKDC, LIG4, XRCC4, XRCC6/Ku70, XRCC5/Ku86 and NHEJ1/XLF. Additional component of the NHEJ complex includes PAXX. Following autophosphorylation, PRKDC dissociates from DNA.SUBCELLULAR LOCATION Autophosphorylated at two clusters, the T2609 cluster and the S2056 cluster. Autophosphorylated on Ser-2055, Thr-2609, Thr-2638 and Thr-2647. Ser-2055 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C (By similarity). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair (By similarity). Autophosphorylation in trans within DNA-PK complexes loaded on DNA ends leads to the dissociation of PRKDC from DNA and the transition into the short-range NHEJ complex (By similarity). Autophosphorylation of the T2609 cluster is required for hematopoietic development and protein synthesis in erythrocytes precursors (By similarity).SIMILARITY Belongs to the PI3/PI4-kinase family.
created [InstanceEdit:217050] Saxena, A, 2008-03-25 20:36:09
crossReference
databaseName UniProt
dbId 217208
description
  • recommendedName: DNA-dependent protein kinase catalytic subunit shortName: DNA-PK catalytic subunit shortName: DNA-PKcs ecNumber evidence="1"2.7.11.1
displayName UniProt:Q8QGX4 PRKDC
geneName
  • PRKDC
  • XRCC7
identifier Q8QGX4
isSequenceChanged false
keyword
  • ATP-binding
  • DNA damage
  • DNA repair
  • Kinase
  • Nucleotide-binding
  • Nucleus
  • Phosphoprotein
  • Reference proteome
  • Repeat
  • Ribosome biogenesis
  • Serine/threonine-protein kinase
  • TPR repeat
  • Transferase
modified [InstanceEdit:9948485] Weiser, Joel, 2025-05-21
moleculeType Protein
name
  • PRKDC
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • PRKDC_CHICK
  • P79791
  • Q9DEI2
sequenceLength 4134
species [Species:49591] Gallus gallus
stId uniprot:Q8QGX4
url http://purl.uniprot.org/uniprot/Q8QGX4
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