UniProt:Q8N684 CPSF7

chain
  • chain:1-471
checksum 69529E441D742CF9
comment
  • FUNCTION Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:17024186, PubMed:29276085, PubMed:8626397). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:17024186, PubMed:8626397). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905, PubMed:29276085). CPSF7 activates directly the mRNA 3'-processing machinery (PubMed:29276085). Binds to pA signals in RNA substrates (PubMed:17024186, PubMed:8626397).SUBUNIT Component of the cleavage factor Im (CFIm) complex which is a heterotetramer composed of two subunits of NUDT21/CPSF5 and two subunits of CPSF6 or CPSF7 or a heterodimer of CPSF6 and CPSF7 (PubMed:20695905, PubMed:23187700, PubMed:8626397, Ref.22). The cleavage factor Im (CFIm) complex associates with the CPSF and CSTF complexes to promote the assembly of the core mRNA 3'-processing machinery (PubMed:29276085). Interacts with NUDT21/CPSF5 (PubMed:29276085). Interacts (via Arg/Ser-rich domain) with FIP1L1 (preferentially via unphosphorylated form and Arg/Glu/Asp-rich region); this interaction mediates, at least in part, the interaction between the CFIm and CPSF complexes and may be inhibited by CPSF7 hyper-phosphorylation (PubMed:29276085).INTERACTION Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460).ALTERNATIVE PRODUCTS Contains an Arg/Ser-rich domain composed of arginine-serine dipeptide repeats within the C-terminal region that is necessary and sufficient for activating mRNA 3'-processing (PubMed:29276085).PTM Phosphorylated (PubMed:29276085).PTM Asymmetrically dimethylated on arginine residues by PRMT1 (PubMed:20562214).SIMILARITY Belongs to the RRM CPSF6/7 family.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Extended N-terminus.
crossReference
databaseName UniProt
dbId 72011
description
  • recommendedName: fullName evidence="14"Cleavage and polyadenylation specificity factor subunit 7 alternativeName: Cleavage and polyadenylation specificity factor 59 kDa subunit shortName: CPSF 59 kDa subunit alternativeName: fullName evidence="13"Cleavage factor Im complex 59 kDa subunit shortName evidence="13"CFIm59 alternativeName: Pre-mRNA cleavage factor Im 59 kDa subunit
displayName UniProt:Q8N684 CPSF7
geneName
  • CPSF7
identifier Q8N684
isSequenceChanged false
keyword
  • 3D-structure
  • Alternative splicing
  • Cytoplasm
  • Isopeptide bond
  • Methylation
  • mRNA processing
  • Nucleus
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • RNA-binding
  • Ubl conjugation
modified [InstanceEdit:9926675] Weiser, Joel, 2024-11-03
moleculeType Protein
name
  • CPSF7
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • CPSF7_HUMAN
  • B3KU04
  • C9K0Q4
  • Q7Z3H9
  • Q9H025
  • Q9H9V1
sequenceLength 471
species [Species:48887] Homo sapiens
stId uniprot:Q8N684
url http://purl.uniprot.org/uniprot/Q8N684
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