UniProt:Q6ZNE5 ATG14

chain
  • chain:1-492
checksum A3EAB0580077D7A6
comment
  • FUNCTION Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604, PubMed:37632749). Modulates the hepatic lipid metabolism (By similarity).SUBUNIT Forms homooligomers; homo-oligomerization is essential for the roles in membrane tethering and enhancement of SNARE-mediated fusion (PubMed:25686604). Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex I (PI3KC3-C1) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with ATG14 (PubMed:18843052, PubMed:19050071, PubMed:19270696, PubMed:22314358, PubMed:23878393). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (PubMed:25490155). PI3KC3-C1 can associate with further regulatory subunits. Interacts with PIK3CB (By similarity). Interacts (via coiled-coil domain) with BECN2 (via coiled-coil domain); this interaction is tighter than BECN2 self-association (PubMed:23954414, PubMed:28218432). Interacts with the STX17-SNAP29 binary t-SNARE complex (PubMed:25686604, PubMed:37632749). Interacts with NRBF2 (By similarity). Interacts with PIK3C3 and BECN1; this interaction is increased in the absence of TMEM39A (PubMed:31806350). Interacts with STEEP1; the interaction is required for trafficking of STING1 from the endoplasmic reticulum (PubMed:32690950). Interacts with ARMC3 (via ARM domains) (By similarity).INTERACTION Cytosolic under nutrient-rich conditions (PubMed:19050071). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:19050071). Accumulates on highly curved PtdIns(3)P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Also localizes to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).ALTERNATIVE PRODUCTS The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.DOMAIN The final 80 residues in the C-terminus define a minimum required region for autophagosome binding called BATS.DOMAIN The N-terminal cysteine repeats are required for proper localization to the endoplasmic reticulum.PTM Ubiquitinated via 'Lys-6', 'Lys-11' and 'Lys-63'-linked polyubiquitin chains on multiple lysines by MARCHF7, leading to ATG14 aggregation and loss of interaction with STX17.SIMILARITY Belongs to the ATG14 family.SEQUENCE CAUTION Extended N-terminus.
created [InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53
crossReference
databaseName UniProt
dbId 233502
description
  • recommendedName: fullName evidence="21"Beclin 1-associated autophagy-related key regulator shortName evidence="21"Barkor alternativeName: fullName evidence="22"Autophagy-related protein 14-like protein shortName evidence="22"Atg14L
displayName UniProt:Q6ZNE5 ATG14
geneName
  • ATG14
  • ATG14L
  • KIAA0831
identifier Q6ZNE5
isSequenceChanged false
keyword
  • 3D-structure
  • Alternative splicing
  • Autophagy
  • Coiled coil
  • Cytoplasm
  • Cytoplasmic vesicle
  • Endoplasmic reticulum
  • Membrane
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • Ubl conjugation
modified [InstanceEdit:9948485] Weiser, Joel, 2025-05-21
moleculeType Protein
name
  • ATG14
otherIdentifier
  • 11725487_at
  • 11725488_a_at
  • 11725489_x_at
  • 11725490_s_at
  • 16793263
  • 204568_PM_at
  • 204568_at
  • 22863
  • 3565740
  • 3565741
  • 3565742
  • 3565743
  • 3565744
  • 3565745
  • 3565746
  • 3565749
  • 3565750
  • 3565753
  • 3565756
  • 3565757
  • 3565758
  • 3565759
  • 3565762
  • 3565763
  • 3565767
  • 3565768
  • 3565775
  • 41372_at
  • 7979328
  • A_23_P309989
  • GE53529
  • GO:0000045
  • GO:0000407
  • GO:0000421
  • GO:0000423
  • GO:0001933
  • GO:0001934
  • GO:0002376
  • GO:0005515
  • GO:0005737
  • GO:0005773
  • GO:0005776
  • GO:0005783
  • GO:0005789
  • GO:0005829
  • GO:0005930
  • GO:0006622
  • GO:0006886
  • GO:0006914
  • GO:0008333
  • GO:0009267
  • GO:0010608
  • GO:0016020
  • GO:0016192
  • GO:0016236
  • GO:0016240
  • GO:0016241
  • GO:0031410
  • GO:0032991
  • GO:0034045
  • GO:0035014
  • GO:0035032
  • GO:0036092
  • GO:0042149
  • GO:0043491
  • GO:0043495
  • GO:0044233
  • GO:0045022
  • GO:0045087
  • GO:0045335
  • GO:0051020
  • GO:0051607
  • GO:0060090
  • GO:0061635
  • GO:0090207
  • GO:0097352
  • GO:0097629
  • GO:0097632
  • GO:0098542
  • GO:0098780
  • GO:0141039
  • HMNXSV003023850
  • ILMN_1678808
  • PH_hs_0033240
  • TC14001159.hg
  • g7662325_3p_at
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • BAKOR_HUMAN
  • A6NJE4
  • A8K9U5
  • B7ZWP5
  • O94920
  • Q32MK7
  • Q32MK8
sequenceLength 492
species [Species:48887] Homo sapiens
stId uniprot:Q6ZNE5
url http://purl.uniprot.org/uniprot/Q6ZNE5
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