UniProt:Q24306 Diap1

chain
  • chain:1-438
checksum 24CA8BC13F5DEF31
comment
  • FUNCTION Anti-apoptotic protein which functions as a caspase regulator, using its E3 ubiquitin-protein ligase activity to smother caspase activity. Binds, ubiquitinates and inactivates initiator caspase Dronc, and effector caspases Drice and Dcp-1. Acts as a Nedd8-E3 ubiquitin-protein ligase for Drice. Suppresses apoptosis by targeting the apoptosome for ubiquitination and inactivation. Plays an important role in cell motility. Overexpression suppresses rpr and hid-dependent cell death in the eye. Interaction of Diap1 with Dronc is required to suppress Dronc-mediated cell death through Diap1-mediated ubiquitination of Dronc. Acts as a positive regulator of Wnt signaling.CATALYTIC ACTIVITY S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.SUBUNIT Interacts (via BIR 2 domain) with Dronc (via residues 114-125) (PubMed:10675329, PubMed:10984473, PubMed:14517550). Rpr, hid and grim can outcompete Dronc for binding Diap1 therefore removing Diap1-mediated ubiquitination (PubMed:10675329, PubMed:14517550). Interacts (via BIR 2 domain) with HtrA2; this displaces any bound Dronc. Interacts with Strica (PubMed:11550090). The N-terminally cleaved form interacts with Ubr3 (via UBR-type zinc finger) (PubMed:25146930, PubMed:26383956); the interaction promotes the recruitment and uniquitination of substrate capases such as Dronc (PubMed:25146930).INTERACTION Detected in wing disks (at protein level).PTM Ubiquitinated and degraded by HtrA2 in apoptotic cells; proteolytic cleavage at specific sites in the BIR domain linker region generating inactive fragments. Mutation of one site reduces but does not abolish cleavage as another site is selected by the protease.SIMILARITY Belongs to the IAP family.SEQUENCE CAUTION Wrong choice of frame.
crossReference
databaseName UniProt
dbId 92747
description
  • recommendedName: Death-associated inhibitor of apoptosis 1 ecNumber evidence="7 10 11"2.3.2.27 alternativeName: Apoptosis 1 inhibitor alternativeName: E3 ubiquitin-protein ligase th alternativeName: Inhibitor of apoptosis 1 alternativeName: Protein thread alternativeName: fullName evidence="16"RING-type E3 ubiquitin transferase Diap1
displayName UniProt:Q24306 Diap1
geneName
  • Diap1
  • Iap1
  • th
  • CG12284
identifier Q24306
isSequenceChanged false
keyword
  • 3D-structure
  • Apoptosis
  • Metal-binding
  • Reference proteome
  • Repeat
  • Transferase
  • Ubl conjugation
  • Ubl conjugation pathway
  • Wnt signaling pathway
  • Zinc
  • Zinc-finger
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
moleculeType Protein
name
  • Diap1
otherIdentifier
  • 152631_at
  • 1636787_s_at
  • 39753
  • GO:0000209
  • GO:0003824
  • GO:0004842
  • GO:0004869
  • GO:0005515
  • GO:0005634
  • GO:0005737
  • GO:0005829
  • GO:0006915
  • GO:0007283
  • GO:0007289
  • GO:0007298
  • GO:0008270
  • GO:0010604
  • GO:0016055
  • GO:0016740
  • GO:0022414
  • GO:0022416
  • GO:0031398
  • GO:0031624
  • GO:0031625
  • GO:0043027
  • GO:0043066
  • GO:0044390
  • GO:0045035
  • GO:0045116
  • GO:0045752
  • GO:0046329
  • GO:0046673
  • GO:0046872
  • GO:0048471
  • GO:0048800
  • GO:0048856
  • GO:0048870
  • GO:0051726
  • GO:0051865
  • GO:0061630
  • GO:0061663
  • GO:0070936
  • GO:0089720
  • GO:0090263
  • GO:0098772
  • GO:0140096
  • GO:1903688
  • GO:1990381
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • DIAP1_DROME
  • A4V1Z9
  • A9UN33
  • Q0E8E4
  • Q8MRM5
  • Q9VUX5
sequenceLength 438
species [Species:56210] Drosophila melanogaster
stId uniprot:Q24306
url http://purl.uniprot.org/uniprot/Q24306
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