Reactome | UniProt:Q16512 PKN1

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UniProt:Q16512 PKN1 Show undefined attributes

chain	initiator methionine:1 chain:2-942
checksum	61360295EC70BB8E
comment	FUNCTION PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)ACTIVITY REGULATION Kinase activity is activated upon binding to Rho proteins (RHOA, RHOB and RAC1). Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by caspase-3 (CASP3) cleavage during apoptosis. Two specific sites, Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif), need to be phosphorylated for its full activation.BIOPHYSICOCHEMICAL PROPERTIES Interacts with ZFAND6 (By similarity). Interacts with AR (PubMed:12514133). Interacts with PRKCB (PubMed:20228790). Interacts (via REM 1 and REM 2 repeats) with RAC1 (PubMed:14514689, PubMed:18006505). Interacts (via REM 1 repeat) with RHOA (PubMed:10619026, PubMed:8571126). Interacts with RHOB (PubMed:9478917). Interacts (via C-terminus) with PDPK1 (PubMed:10792047). Interacts with CCNT2; enhances MYOD1-dependent transcription (PubMed:16331689). Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (PubMed:21224381).SUBUNIT (Microbial infection) Interacts (via the second REM repeat) with S.typhimurium E3 ubiquitin-protein ligase SspH1 (via the leucine-rich repeat region) (PubMed:16611232, PubMed:24248594).INTERACTION Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.ALTERNATIVE PRODUCTS Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.DOMAIN The C1 domain does not bind the diacylglycerol (DAG).PTM Autophosphorylated; preferably on serine. Phosphorylated during mitosis.PTM Activated by limited proteolysis with trypsin.PTM (Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.SIMILARITY Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
crossReference	[DatabaseIdentifier:11481967] ENSEMBL:ENSG00000123143 [DatabaseIdentifier:12818556] ZINC - World Drugs:PKN1_HUMAN [DatabaseIdentifier:12815670] ZINC target:Q16512 [DatabaseIdentifier:12812307] ZINC - Substances:PKN1_HUMAN [DatabaseIdentifier:12810527] ZINC - Predictions - Purchasable:PKN1_HUMAN [DatabaseIdentifier:12807638] ZINC - Metabolites:PKN1_HUMAN [DatabaseIdentifier:12807001] ZINC - Investigational:PKN1_HUMAN [DatabaseIdentifier:12805918] ZINC - FDA approved:PKN1_HUMAN [DatabaseIdentifier:12804839] ZINC - Biogenic:PKN1_HUMAN [DatabaseIdentifier:12388981] RefSeq:NP_002732.3 [DatabaseIdentifier:12388980] RefSeq:NP_998725.1 [DatabaseIdentifier:12292227] Pharos - Targets:Q16512 [DatabaseIdentifier:12275180] PRO:Q16512 [DatabaseIdentifier:12221992] PDB:4OTG [DatabaseIdentifier:12221995] PDB:1URF [DatabaseIdentifier:12177974] PDB:1CXZ [DatabaseIdentifier:12221994] PDB:4OTI [DatabaseIdentifier:12205062] PDB:4NKG [DatabaseIdentifier:12221991] PDB:4OTD [DatabaseIdentifier:12165541] PDB:2RMK [DatabaseIdentifier:12221993] PDB:4OTH [DatabaseIdentifier:12132044] OpenTargets:ENSG00000123143 [DatabaseIdentifier:11847368] HPA:ENSG00000123143-PKN1 [DatabaseIdentifier:11831411] HMDB Protein:HMDBP01531 [DatabaseIdentifier:11810342] GlyGen:Q16512 [DatabaseIdentifier:11794395] GeneCards:PKN1 [DatabaseIdentifier:11780951] Guide to Pharmacology - Targets:1520 [DatabaseIdentifier:11706017] ENSEMBL:ENST00000242783 [DatabaseIdentifier:11706018] ENSEMBL:ENST00000342216 [DatabaseIdentifier:11583319] ENSEMBL:ENSP00000343325 [DatabaseIdentifier:11583318] ENSEMBL:ENSP00000242783
databaseName	UniProt
dbId	61782
description	recommendedName: Serine/threonine-protein kinase N1 ecNumber evidence="22"2.7.11.13 alternativeName: Protease-activated kinase 1 shortName: PAK-1 alternativeName: Protein kinase C-like 1 alternativeName: Protein kinase C-like PKN alternativeName: Protein kinase PKN-alpha alternativeName: Protein-kinase C-related kinase 1 alternativeName: Serine-threonine protein kinase N
displayName	UniProt:Q16512 PKN1
geneName	PKN1 PAK1 PKN PRK1 PRKCL1
identifier	Q16512
isSequenceChanged	false
keyword	3D-structure Acetylation Alternative splicing ATP-binding Cell membrane Chromatin regulator Coiled coil Cytoplasm Endosome Kinase Membrane Nucleotide-binding Nucleus Phosphoprotein Proteomics identification Reference proteome Repeat Serine/threonine-protein kinase Transcription Transcription regulation Transferase Ubl conjugation
modified	[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
moleculeType	Protein
name	PKN1
otherIdentifier	11733072_a_at 16858991 175_s_at 202161_PM_at 202161_at 3822724 3822725 3822726 3822728 3822730 3822731 3822732 3822733 3822734 3822735 3822738 3822739 3822740 3822744 3822745 3822746 3822747 3822749 3822752 3822753 3822754 3822755 3822756 3822757 3822758 3822759 3822760 3822761 3822762 3822763 3822764 3822765 3822766 3822768 3822770 3822771 3822772 3822773 3822775 3822776 3822778 3822780 3822781 5585 8026315 80688_at A_14_P134902 A_23_P67271 GE59091 GO:0000166 GO:0001782 GO:0001783 GO:0002376 GO:0002634 GO:0002637 GO:0003014 GO:0003682 GO:0003713 GO:0003824 GO:0004672 GO:0004674 GO:0004697 GO:0005080 GO:0005515 GO:0005524 GO:0005634 GO:0005654 GO:0005737 GO:0005768 GO:0005829 GO:0005886 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006468 GO:0006972 GO:0007165 GO:0010631 GO:0016020 GO:0016301 GO:0016740 GO:0030496 GO:0030889 GO:0031267 GO:0031410 GO:0032154 GO:0032991 GO:0035402 GO:0035556 GO:0042393 GO:0042826 GO:0043687 GO:0045893 GO:0048536 GO:0048856 GO:0048870 GO:0050681 GO:0060765 GO:0106310 GO:0140096 GO:2000145 ILMN_1662687 ILMN_2272120 ILMN_2367707 ILMN_2367710 PH_hs_0000335 TC19000257.hg U33053_at g4506072_3p_at p8579
physicalEntity	[EntityWithAccessionedSequence:R-HSA-5623651] p-T774-PKN1 [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-5623613] PKN1 [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-5625721] p-T774-PKN1 [nucleoplasm] - Homo sapiens
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:ensembl:ENSG00000123143] ENSEMBL:ENSG00000123143 PKN1 [ReferenceDNASequence:12693817] UCSC:Q16512 PKN1 [ReferenceDNASequence:12116438] OMIM:601032 PKN1 [ReferenceDNASequence:12061290] NCBI Gene:5585 PKN1 [ReferenceDNASequence:11977197] Monarch:5585 PKN1 [ReferenceDNASequence:11887435] KEGG:hsa:5585 PKN1 [ReferenceDNASequence:11826091] HGNC:9405 PKN1 [ReferenceDNASequence:11429152] dbSNP Gene:5585 PKN1 [ReferenceDNASequence:11423135] CTD Gene:5585 PKN1 [ReferenceDNASequence:11393215] COSMIC - genes:PAK1 PKN1 [ReferenceDNASequence:11393214] COSMIC - genes:PKN1 PKN1 [ReferenceDNASequence:11347408] BioGPS Gene:5585 PKN1
referenceTranscript	[ReferenceRNASequence:12570765] RefSeq:NM_213560.3 PKN1 [ReferenceRNASequence:12570766] RefSeq:NM_002741.3 PKN1
schemaClass	ReferenceGeneProduct
secondaryIdentifier	PKN1_HUMAN A8K7W5 B2R9R4 B3KVN3 Q15143 Q504U4 Q8IUV5 Q9UD44
sequenceLength	942
species	[Species:48887] Homo sapiens
stId	uniprot:Q16512
url	http://purl.uniprot.org/uniprot/Q16512

Referrals

(referenceEntity)	[EntityWithAccessionedSequence:R-HSA-5623651] p-T774-PKN1 [cytosol] [EntityWithAccessionedSequence:R-HSA-5623613] PKN1 [cytosol] [EntityWithAccessionedSequence:R-HSA-5625721] p-T774-PKN1 [nucleoplasm]
(referenceSequence)	[ModifiedResidue:5623657] O-phospho-L-threonine at 774
(interactor)	[UndirectedInteraction:11281519] UniProt:Q16512 <-> UniProt:Q15834 (IntAct) [UndirectedInteraction:11281517] UniProt:Q16512 <-> UniProt:P43363 (IntAct) [UndirectedInteraction:11281515] UniProt:Q16512 <-> UniProt:Q13064 (IntAct) [UndirectedInteraction:11281513] UniProt:Q16512 <-> UniProt:Q92764 (IntAct) [UndirectedInteraction:11281511] UniProt:Q16512 <-> UniProt:Q96MN9-2 (IntAct) [UndirectedInteraction:11281509] UniProt:Q16512 <-> UniProt:Q8WTV1 (IntAct) [UndirectedInteraction:11281507] UniProt:Q16512 <-> UniProt:Q8NHQ1 (IntAct) [UndirectedInteraction:11281505] UniProt:Q16512 <-> UniProt:Q8N108-16 (IntAct) [UndirectedInteraction:11281503] UniProt:Q16512 <-> UniProt:O76013-2 (IntAct) [UndirectedInteraction:11281501] UniProt:Q16512 <-> UniProt:Q96QF0-7 (IntAct) [UndirectedInteraction:11281499] UniProt:Q16512 <-> UniProt:O15344 (IntAct) [UndirectedInteraction:11281497] UniProt:Q16512 <-> UniProt:Q9H2G9 (IntAct) [UndirectedInteraction:11281495] UniProt:Q16512 <-> UniProt:Q9P0T4 (IntAct) [UndirectedInteraction:11281493] UniProt:Q16512 <-> UniProt:Q9UJV3-2 (IntAct) [UndirectedInteraction:11281491] UniProt:Q16512 <-> IntAct:EBI-22616457 (IntAct) [UndirectedInteraction:11281489] UniProt:Q16512 <-> UniProt:Q5TA45 (IntAct) [UndirectedInteraction:11281487] UniProt:Q16512 <-> UniProt:Q9NRD5 (IntAct) [UndirectedInteraction:11281485] UniProt:Q16512 <-> UniProt:Q96CS2 (IntAct) [UndirectedInteraction:11281483] UniProt:Q16512 <-> UniProt:Q9ULX6 (IntAct) [UndirectedInteraction:11281481] UniProt:Q16512 <-> UniProt:Q8ND90 (IntAct) [UndirectedInteraction:11281479] UniProt:Q16512 <-> UniProt:P08779 (IntAct) [UndirectedInteraction:11281477] UniProt:Q16512 <-> UniProt:Q96R06 (IntAct) [UndirectedInteraction:11281475] UniProt:Q16512 <-> UniProt:Q5JR59-3 (IntAct) [UndirectedInteraction:11281473] UniProt:Q16512 <-> UniProt:Q96M89-2 (IntAct) [UndirectedInteraction:11281469] UniProt:Q16512 <-> UniProt:Q8WU43 (IntAct) [UndirectedInteraction:11281467] UniProt:Q16512 <-> UniProt:Q9BV99 (IntAct) [UndirectedInteraction:11281465] UniProt:Q16512 <-> UniProt:Q9NRI5-2 (IntAct) [UndirectedInteraction:11281463] UniProt:Q16512 <-> UniProt:Q8IWV8-2 (IntAct) [UndirectedInteraction:11281459] UniProt:Q16512 <-> UniProt:Q8TF47 (IntAct) [UndirectedInteraction:11281457] UniProt:Q16512 <-> UniProt:Q6UY14-3 (IntAct) [UndirectedInteraction:11281455] UniProt:Q16512 <-> UniProt:Q9H7B4 (IntAct) [UndirectedInteraction:11281453] UniProt:Q16512 <-> UniProt:Q9UNY5 (IntAct) [UndirectedInteraction:11281451] UniProt:Q16512 <-> UniProt:A8MQ03 (IntAct) [UndirectedInteraction:11281449] UniProt:Q16512 <-> UniProt:Q86T90 (IntAct) [UndirectedInteraction:11133849] UniProt:P08670 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11157913] UniProt:P14373 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11175821] UniProt:Q9UGI0 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11185831] UniProt:P26641 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11189133] UniProt:A1L190 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11198001] UniProt:Q08379 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11206861] UniProt:P28070 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11245005] UniProt:Q12933 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11259139] UniProt:P07196 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11274023] UniProt:P05067 <-> UniProt:Q16512 (IntAct) [UndirectedInteraction:11281385] UniProt:Q16512 <-> UniProt:Q9NSC5 (IntAct) [UndirectedInteraction:11281387] UniProt:Q16512 <-> UniProt:Q01850 (IntAct) [UndirectedInteraction:11281389] UniProt:Q16512 <-> UniProt:Q15323 (IntAct) [UndirectedInteraction:11281391] UniProt:Q16512 <-> UniProt:Q14457 (IntAct) [UndirectedInteraction:11281393] UniProt:Q16512 <-> UniProt:P08727 (IntAct) [UndirectedInteraction:11281395] UniProt:Q16512 <-> UniProt:Q9UJW9 (IntAct) [UndirectedInteraction:11281397] UniProt:Q16512 <-> UniProt:P19012 (IntAct) [UndirectedInteraction:11281399] UniProt:Q16512 <-> UniProt:A6NEM1 (IntAct) [UndirectedInteraction:11281401] UniProt:Q16512 <-> UniProt:Q8IW40 (IntAct) [UndirectedInteraction:11281403] UniProt:Q16512 <-> UniProt:Q9BZW7 (IntAct) [UndirectedInteraction:11281405] UniProt:Q16512 <-> UniProt:O76014 (IntAct) [UndirectedInteraction:11281407] UniProt:Q16512 <-> IntAct:EBI-16435328 (IntAct) [UndirectedInteraction:11281409] UniProt:Q16512 <-> UniProt:O00303 (IntAct) [UndirectedInteraction:11281411] UniProt:Q16512 <-> UniProt:Q9NZ72 (IntAct) [UndirectedInteraction:11281413] UniProt:Q16512 <-> UniProt:Q06547-2 (IntAct) [UndirectedInteraction:11281415] UniProt:Q16512 <-> UniProt:Q9Y2D8 (IntAct) [UndirectedInteraction:11281417] UniProt:Q16512 <-> UniProt:P15289 (IntAct) [UndirectedInteraction:11281419] UniProt:Q16512 <-> UniProt:O76011 (IntAct) [UndirectedInteraction:11281421] UniProt:Q16512 <-> UniProt:Q8N6Y0 (IntAct) [UndirectedInteraction:11281423] UniProt:Q16512 <-> UniProt:Q8IV13 (IntAct) [UndirectedInteraction:11281425] UniProt:Q16512 <-> UniProt:Q9UKT9 (IntAct) [UndirectedInteraction:11281427] UniProt:Q16512 <-> UniProt:Q13077 (IntAct) [UndirectedInteraction:11281429] UniProt:Q16512 <-> UniProt:P54257 (IntAct) [UndirectedInteraction:11281431] UniProt:Q16512 <-> UniProt:Q86XR8-3 (IntAct) [UndirectedInteraction:11281433] UniProt:Q16512 <-> UniProt:Q8TD10 (IntAct) [UndirectedInteraction:11281435] UniProt:Q16512 <-> UniProt:Q8WWB3 (IntAct) [UndirectedInteraction:11281437] UniProt:Q16512 <-> UniProt:Q8IYX8-2 (IntAct) [UndirectedInteraction:11281439] UniProt:Q16512 <-> UniProt:P78424 (IntAct) [UndirectedInteraction:11281441] UniProt:Q16512 <-> UniProt:O75558 (IntAct) [UndirectedInteraction:11281443] UniProt:Q16512 <-> UniProt:Q8N5Z5 (IntAct) [UndirectedInteraction:11281445] UniProt:Q16512 <-> UniProt:Q6PJH3 (IntAct) [UndirectedInteraction:11281447] UniProt:Q16512 <-> UniProt:O75031 (IntAct)

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