Reactome | UniProt:P63280 Ube2i

chain	initiator methionine:1 chain:2-158
checksum	E2C826E9C8D0683D
comment	FUNCTION Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5 (By similarity). Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (By similarity). Sumoylates SHMT1 at 'Lys-32' or 'Lys-33' leading to RAN-dependent nuclear import of SHMT1 (By similarity). Also sumoylates TYMS and DHFR (By similarity).PATHWAY Protein modification; protein sumoylation.SUBUNIT Forms a complex with SENP6 and UBE2I in response to UV irradiation (By similarity). Forms a tight complex with RANGAP1 and RANBP2 (By similarity). Identified in a complex with SUMO2 and UBE2I, where one ZNF451 interacts with one UBE2I and two SUMO2 chains, one bound to the UBE2I active site and the other to another region of the same UBE2I molecule (By similarity). Interacts with SETX (By similarity). Interacts with HIPK1 and HIPK2 (PubMed:10535925). Interacts with PPM1J (PubMed:12633878). Interacts with RASD2 (By similarity). Interacts with TCF3 (PubMed:9409784). Interacts with NR2C1; the interaction promotes its sumoylation (PubMed:17187077). Interacts with SIAH1 (By similarity). Interacts with PARP (By similarity). Interacts with various transcription factors such as TFAP2A, TFAP2B, and TFAP2C (By similarity). Interacts with AR (By similarity). Interacts with ETS1 (By similarity). Interacts with SOX4 (By similarity). Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B (By similarity). Interacts with FOXL2 (By similarity). Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain (By similarity). Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains (By similarity). Interacts with FHIT (By similarity). Interacts with PRKRA and p53/TP53 (PubMed:22214662). Interacts with UHRF2 (By similarity). Interacts with NR3C1 and this interaction is enhanced in the presence of RWDD3 (By similarity). Interacts with MTA1 (By similarity). Interacts with ZNF451 (By similarity). Interacts with CPEB3 (PubMed:26074071). Interacts with SUMO1, SUMO2, and SUMO3 (By similarity). Interacts with IPO13 (By similarity). Interacts with DNMT1 (By similarity).INTERACTION Mainly nuclear (PubMed:18681895). In spermatocytes, localizes in synaptonemal complexes (By similarity). Recruited by BCL11A into the nuclear body (PubMed:18681895).TISSUE SPECIFICITY Present in spleen, kidney, lung, brain, heart and testis (at protein level).PTM Phosphorylation at Ser-71 significantly enhances SUMOylation activity.DISRUPTION PHENOTYPE Death prior to E7.5 due to major defects in nuclear organization.SIMILARITY Belongs to the ubiquitin-conjugating enzyme family.
crossReference	[DatabaseIdentifier:11533726] ENSEMBL:ENSMUSP00000133311 [DatabaseIdentifier:12313121] RefSeq:XP_036016378.1 [DatabaseIdentifier:12313119] RefSeq:XP_006524112.1 [DatabaseIdentifier:12313124] RefSeq:NP_035795.1 [DatabaseIdentifier:12313122] RefSeq:NP_001171080.1 [DatabaseIdentifier:12313123] RefSeq:NP_001171081.1 [DatabaseIdentifier:12313120] RefSeq:NP_001344623.1 [DatabaseIdentifier:12240543] PRO:P63280 [DatabaseIdentifier:12159641] PDB:2UYZ [DatabaseIdentifier:12159640] PDB:1U9A [DatabaseIdentifier:12159639] PDB:1U9B [DatabaseIdentifier:12159642] PDB:2VRR [DatabaseIdentifier:11930732] MGI:107365 [DatabaseIdentifier:11656430] ENSEMBL:ENSMUST00000173713 [DatabaseIdentifier:11656438] ENSEMBL:ENSMUST00000173084 [DatabaseIdentifier:11656431] ENSEMBL:ENSMUST00000249707 [DatabaseIdentifier:11656426] ENSEMBL:ENSMUST00000173231 [DatabaseIdentifier:11656433] ENSEMBL:ENSMUST00000249703 [DatabaseIdentifier:11656446] ENSEMBL:ENSMUST00000249684 [DatabaseIdentifier:11656441] ENSEMBL:ENSMUST00000249701 [DatabaseIdentifier:11656443] ENSEMBL:ENSMUST00000249700 [DatabaseIdentifier:11656442] ENSEMBL:ENSMUST00000249689 [DatabaseIdentifier:11656440] ENSEMBL:ENSMUST00000173621 [DatabaseIdentifier:11656444] ENSEMBL:ENSMUST00000249688 [DatabaseIdentifier:11656449] ENSEMBL:ENSMUST00000249681 [DatabaseIdentifier:11656447] ENSEMBL:ENSMUST00000249683 [DatabaseIdentifier:11656448] ENSEMBL:ENSMUST00000249682 [DatabaseIdentifier:11656435] ENSEMBL:ENSMUST00000249698 [DatabaseIdentifier:11656425] ENSEMBL:ENSMUST00000172462 [DatabaseIdentifier:11656434] ENSEMBL:ENSMUST00000249699 [DatabaseIdentifier:11656427] ENSEMBL:ENSMUST00000174001 [DatabaseIdentifier:11656429] ENSEMBL:ENSMUST00000049911 [DatabaseIdentifier:11656445] ENSEMBL:ENSMUST00000249686 [DatabaseIdentifier:11656428] ENSEMBL:ENSMUST00000172520 [DatabaseIdentifier:11656432] ENSEMBL:ENSMUST00000249705 [DatabaseIdentifier:11656436] ENSEMBL:ENSMUST00000249695 [DatabaseIdentifier:11656437] ENSEMBL:ENSMUST00000249690 [DatabaseIdentifier:11656439] ENSEMBL:ENSMUST00000174031 [DatabaseIdentifier:11533736] ENSEMBL:ENSMUSP00000159870 [DatabaseIdentifier:11533728] ENSEMBL:ENSMUSP00000159864 [DatabaseIdentifier:11533748] ENSEMBL:ENSMUSP00000159861 [DatabaseIdentifier:11446377] ENSEMBL:ENSMUSG00000015120 [DatabaseIdentifier:11533732] ENSEMBL:ENSMUSP00000134491 [DatabaseIdentifier:11533733] ENSEMBL:ENSMUSP00000134450 [DatabaseIdentifier:11533741] ENSEMBL:ENSMUSP00000159879 [DatabaseIdentifier:11533743] ENSEMBL:ENSMUSP00000159877 [DatabaseIdentifier:11533745] ENSEMBL:ENSMUSP00000159859 [DatabaseIdentifier:11533747] ENSEMBL:ENSMUSP00000159860 [DatabaseIdentifier:11533738] ENSEMBL:ENSMUSP00000159873 [DatabaseIdentifier:11533731] ENSEMBL:ENSMUSP00000133338 [DatabaseIdentifier:11533744] ENSEMBL:ENSMUSP00000159856 [DatabaseIdentifier:11533729] ENSEMBL:ENSMUSP00000159865 [DatabaseIdentifier:11533746] ENSEMBL:ENSMUSP00000134161 [DatabaseIdentifier:11533739] ENSEMBL:ENSMUSP00000159874 [DatabaseIdentifier:11533740] ENSEMBL:ENSMUSP00000159857 [DatabaseIdentifier:11533750] ENSEMBL:ENSMUSP00000159881 [DatabaseIdentifier:11533737] ENSEMBL:ENSMUSP00000159875 [DatabaseIdentifier:11533727] ENSEMBL:ENSMUSP00000055714 [DatabaseIdentifier:11533734] ENSEMBL:ENSMUSP00000159872 [DatabaseIdentifier:11533742] ENSEMBL:ENSMUSP00000159858 [DatabaseIdentifier:11533730] ENSEMBL:ENSMUSP00000159863 [DatabaseIdentifier:11533735] ENSEMBL:ENSMUSP00000134350 [DatabaseIdentifier:11533749] ENSEMBL:ENSMUSP00000134261
databaseName	UniProt
dbId	66765
description	recommendedName: SUMO-conjugating enzyme UBC9 ecNumber: 2.3.2.- alternativeName: RING-type E3 SUMO transferase UBC9 alternativeName: SUMO-protein ligase alternativeName: Ubiquitin carrier protein 9 shortName: mUBC9 alternativeName: Ubiquitin carrier protein I alternativeName: Ubiquitin-conjugating enzyme E2 I alternativeName: Ubiquitin-protein ligase I
displayName	UniProt:P63280 Ube2i
geneName	Ube2i Ubc9 Ubce2i Ubce9
identifier	P63280
isSequenceChanged	false
keyword	3D-structure Acetylation ATP-binding Cell cycle Cell division Chromosome partition Cytoplasm Developmental protein Host-virus interaction Isopeptide bond Mitosis Nucleotide-binding Nucleus Phosphoprotein Reference proteome Transferase Ubl conjugation Ubl conjugation pathway
modified	[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType	Protein
name	Ube2i
otherIdentifier	10448912 10478012 10598636 107897_at 133474_at 1422712_a_at 1422713_a_at 1422714_at 1425478_x_at 1429545_at 1436457_at 1453189_at 17342219 22196 4363964 4371453 4393366 4417409 4486178 4517816 4549465 4559775 4588862 4596401 4630922 4636767 4759668 4764201 4771206 4897603 4901620 4920336 4943594 4971430 4973579 4988327 4996370 5000153 5097726 5157053 5173224 5230168 5246213 5270223 5319148 5360075 5441357 5605693 97530_at A_51_P366406 A_51_P400909 A_52_P1067 A_52_P269595 A_52_P297731 A_52_P369112 A_55_P1980543 A_55_P1984721 A_55_P2114915 A_55_P2326517 A_66_P132625 GE36858 GO:0000122 GO:0000166 GO:0001221 GO:0003824 GO:0005515 GO:0005524 GO:0005634 GO:0005643 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006355 GO:0006913 GO:0007059 GO:0016020 GO:0016604 GO:0016605 GO:0016740 GO:0016925 GO:0019787 GO:0019789 GO:0019899 GO:0030335 GO:0036211 GO:0043123 GO:0043398 GO:0044388 GO:0045892 GO:0050804 GO:0051168 GO:0051301 GO:0061656 GO:0071535 GO:0098685 GO:0098978 GO:0099523 GO:0099524 GO:0106068 GO:0140096 GO:1990234 ILMN_2417991 ILMN_2499058 ILMN_2518142 ILMN_2786360 mMC019519 mMR028357 mMR028452
physicalEntity	[EntityWithAccessionedSequence:R-MMU-3730619] Ube2i [nucleoplasm] - Mus musculus [EntityWithAccessionedSequence:R-MMU-912483] Ube2i [nucleoplasm] - Mus musculus [EntityWithAccessionedSequence:R-MMU-3730617] Ube2i [nucleoplasm] - Mus musculus [EntityWithAccessionedSequence:R-MMU-4656909] Ube2i [cytosol] - Mus musculus [EntityWithAccessionedSequence:R-MMU-4655403] Ube2i [cytosol] - Mus musculus [EntityWithAccessionedSequence:R-MMU-4656944] Ube2i [cytosol] - Mus musculus [EntityWithAccessionedSequence:R-MMU-3730629] Ube2i [nucleoplasm] - Mus musculus [EntityWithAccessionedSequence:R-MMU-4655408] Ube2i [nuclear envelope] - Mus musculus [EntityWithAccessionedSequence:R-MMU-3730632] Sumo1-C93-Ube2i [nucleoplasm] - Mus musculus [EntityWithAccessionedSequence:R-MMU-3730779] Sumo3-C93-Ube2i [nucleoplasm] - Mus musculus [EntityWithAccessionedSequence:R-MMU-3730635] Sumo2-C93-Ube2i [nucleoplasm] - Mus musculus [EntityWithAccessionedSequence:R-MMU-4085358] Ube2i [nucleoplasm] - Mus musculus
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:11312013] BioGPS Gene:22196 Ube2i [ReferenceDNASequence:12670820] UCSC:P63280 Ube2i [ReferenceDNASequence:12025895] NCBI Gene:22196 Ube2i [ReferenceDNASequence:11941802] Monarch:22196 Ube2i [ReferenceDNASequence:11855647] KEGG:mmu:22196 Ube2i
referenceTranscript	[ReferenceRNASequence:12495177] RefSeq:NM_001177610.1 Ube2i [ReferenceRNASequence:12495176] RefSeq:XM_006524049.5 Ube2i [ReferenceRNASequence:12495179] RefSeq:NM_001357694.1 Ube2i [ReferenceRNASequence:12495175] RefSeq:NM_001177609.1 Ube2i [ReferenceRNASequence:12495178] RefSeq:XM_036160485.1 Ube2i [ReferenceRNASequence:12495180] RefSeq:NM_011665.4 Ube2i
schemaClass	ReferenceGeneProduct
secondaryIdentifier	UBC9_MOUSE P50550 Q15698 Q6RUT3 Q86VB3
sequenceLength	158
species	[Species:48892] Mus musculus
stId	uniprot:P63280
url	http://purl.uniprot.org/uniprot/P63280

(referenceEntity)	[EntityWithAccessionedSequence:R-MMU-3730619] Ube2i [nucleoplasm] [EntityWithAccessionedSequence:R-MMU-912483] Ube2i [nucleoplasm] [EntityWithAccessionedSequence:R-MMU-3730617] Ube2i [nucleoplasm] [EntityWithAccessionedSequence:R-MMU-4656909] Ube2i [cytosol] [EntityWithAccessionedSequence:R-MMU-4655403] Ube2i [cytosol] [EntityWithAccessionedSequence:R-MMU-4656944] Ube2i [cytosol] [EntityWithAccessionedSequence:R-MMU-3730629] Ube2i [nucleoplasm] [EntityWithAccessionedSequence:R-MMU-4655408] Ube2i [nuclear envelope] [EntityWithAccessionedSequence:R-MMU-3730632] Sumo1-C93-Ube2i [nucleoplasm] [EntityWithAccessionedSequence:R-MMU-3730779] Sumo3-C93-Ube2i [nucleoplasm] [EntityWithAccessionedSequence:R-MMU-3730635] Sumo2-C93-Ube2i [nucleoplasm] [EntityWithAccessionedSequence:R-MMU-4085358] Ube2i [nucleoplasm]
(referenceSequence)	[InterChainCrosslinkedResidue:10046936] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 93 and 93 (in Homo sapiens) [InterChainCrosslinkedResidue:10046781] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 93 and 97 (in Homo sapiens) [InterChainCrosslinkedResidue:10046944] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 93 and 92 (in Homo sapiens) [InterChainCrosslinkedResidue:3232132] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 93 and 97 [InterChainCrosslinkedResidue:3232125] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 93 and 92 [InterChainCrosslinkedResidue:3214919] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 93 and 93 [GroupModifiedResidue:10050344] sumoylated lysine (monoSUMO1 [nucleoplasm]) at 14 (in Homo sapiens)
(secondReferenceSequence)	[InterChainCrosslinkedResidue:3730633] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 97 and 93 [InterChainCrosslinkedResidue:3730773] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 92 and 93 [InterChainCrosslinkedResidue:3730636] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 93 and 93
(interactor)	[UndirectedInteraction:11145133] UniProt:P08152 <-> UniProt:P63280 (IntAct) [UndirectedInteraction:11161055] UniProt:Q505F1 <-> UniProt:P63280 (IntAct)

UniProt:P63280 Ube2i Show undefined attributes

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