UniProt:P33991 MCM4

chain
  • initiator methionine:1
  • chain:2-863
checksum 96D9CA2A7D88D015
comment
  • FUNCTION Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:9305914).CATALYTIC ACTIVITY ATP + H2O = ADP + phosphate + H(+)SUBUNIT Component of the MCM2-7 complex (PubMed:16899510, PubMed:9305914). The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (PubMed:16899510, PubMed:32453425, PubMed:9305914). Component of the CMG helicase complex, a hexameric ring of related MCM2-7 subunits stabilized by CDC45 and the tetrameric GINS complex (PubMed:32453425, PubMed:34694004, PubMed:34700328). Interacts with MCMBP (PubMed:17296731).INTERACTION Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.PTM Sumoylated; SUMO2 modified in response to stress caused by inhibition of proteasome activity (in vitro).DISEASE The disease is caused by variants affecting the gene represented in this entry.MISCELLANEOUS Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.SIMILARITY Belongs to the MCM family.
crossReference
databaseName UniProt
dbId 94950
description
  • recommendedName: DNA replication licensing factor MCM4 ecNumber evidence="1"3.6.4.12 alternativeName: CDC21 homolog alternativeName: P1-CDC21
displayName UniProt:P33991 MCM4
geneName
  • MCM4
  • CDC21
identifier P33991
isSequenceChanged false
keyword
  • 3D-structure
  • Acetylation
  • ATP-binding
  • Cell cycle
  • Chromosome
  • DNA replication
  • DNA-binding
  • Helicase
  • Hydrolase
  • Isopeptide bond
  • Nucleotide-binding
  • Nucleus
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • Ubl conjugation
modified [InstanceEdit:9963647] Weiser, Joel, 2025-08-15
moleculeType Protein
name
  • MCM4
otherIdentifier
  • 11736670_a_at
  • 11743692_a_at
  • 11743693_a_at
  • 11759509_s_at
  • 11759510_x_at
  • 17068782
  • 17068812
  • 212141_PM_at
  • 212141_at
  • 212142_PM_at
  • 212142_at
  • 222036_PM_s_at
  • 222036_s_at
  • 222037_PM_at
  • 222037_at
  • 242479_PM_s_at
  • 242479_s_at
  • 3097154
  • 3097155
  • 3097156
  • 3097157
  • 3097158
  • 3097159
  • 3097160
  • 3097161
  • 3097162
  • 3097163
  • 3097164
  • 3097165
  • 3097166
  • 3097167
  • 3097168
  • 3097169
  • 3097170
  • 3097171
  • 3097172
  • 3097173
  • 3097175
  • 3097176
  • 3097177
  • 3097178
  • 3097179
  • 3097180
  • 3097181
  • 3097182
  • 3097183
  • 3097184
  • 3097185
  • 3097186
  • 3097187
  • 3097188
  • 3097189
  • 3097190
  • 3097191
  • 3097192
  • 3097193
  • 3097194
  • 3097195
  • 3097196
  • 3097197
  • 3097198
  • 3097199
  • 3097200
  • 3097201
  • 3097202
  • 3097203
  • 3097205
  • 4173
  • 42875_r_at
  • 52597_at
  • 63855_r_at
  • 8146357
  • 8146377
  • 981_at
  • A_14_P102189
  • A_14_P118280
  • A_14_P118623
  • A_14_P134350
  • A_23_P370989
  • GO:0000166
  • GO:0000727
  • GO:0000781
  • GO:0003677
  • GO:0003678
  • GO:0003697
  • GO:0003824
  • GO:0004386
  • GO:0005515
  • GO:0005524
  • GO:0005634
  • GO:0005654
  • GO:0005694
  • GO:0006260
  • GO:0006270
  • GO:0006271
  • GO:0006281
  • GO:0006310
  • GO:0016020
  • GO:0016787
  • GO:0016887
  • GO:0017116
  • GO:0030174
  • GO:0042555
  • GO:0071162
  • GO:0140097
  • GO:0140657
  • GO:1902975
  • HMNXSV003008366
  • Hs.154443.0.S1_3p_at
  • Hs.154443.0.S2_3p_at
  • Hs.154443.1.S1_3p_a_at
  • Hs.154443.1.S1_3p_x_at
  • Hs.296277.0.S1_3p_s_at
  • ILMN_1737205
  • ILMN_2412860
  • PH_hs_0026403
  • TC08000346.hg
  • TC08000347.hg
  • X74794_at
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • MCM4_HUMAN
  • Q8NEH1
  • Q99658
sequenceLength 863
species [Species:48887] Homo sapiens
stId uniprot:P33991
url http://purl.uniprot.org/uniprot/P33991

Referrals

(referenceEntity)
(interactor)
Cite Us!