UniProt:P33755 NPL4

chain
  • chain:1-580
checksum 6EF35D21338EE07E
comment
  • FUNCTION Substrate-recruiting cofactor of the CDC48-NPL4-UFD1 segregase (PubMed:31249135). Assists CDC48 in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation (PubMed:11739805, PubMed:11740563, PubMed:11847109). Involved in the import of nuclear-targeted proteins into the nucleus and the export of poly(A) RNA out of the nucleus (PubMed:11733065, PubMed:8930904). Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1 (PubMed:11733065). Regulates ubiquitin-mediated mitochondria protein degradation (PubMed:31249135). Involved in spindle disassembly probably by promoting the degradation of spindle assembly factors ASE1 and CDC5 at the end of mitosis (PubMed:14636562).SUBUNIT Component of the heterotrimeric CDC48-NPL4-UFD1 ATPase complex (PubMed:16873066). The CDC48-NPL4-UFD1 ATPase complex interacts with the HRD1 ubiquitin ligase complex composed of the E3 ligase HRD1, its cofactors HRD3, USA1 and DER1, substrate recruiting factor YOS9 and CDC48-binding protein UBX2 (PubMed:16873066). Interaction between the complexes is mediated by interaction between CDC48-NPL4-UFD1 complex member CDC48 and HRD1 complex member UBX2 (PubMed:16873066). Forms a complex composed of CDC48, NPL4, UFD1, DOA1, SHP1 and deubiquitinase OTU1 (PubMed:16427015). Interacts with CDC48, UFD1 and VMS1 (PubMed:11598205, PubMed:11733065, PubMed:11739805, PubMed:21070972, PubMed:31249135).INTERACTION Localizes mainly at the nuclear periphery and the endoplasmic reticulum membrane.MISCELLANEOUS Present with 1050 molecules/cell in log phase SD medium.SIMILARITY Belongs to the NPL4 family.
crossReference
databaseName UniProt
dbId 96537
description
  • recommendedName: Nuclear protein localization protein 4 alternativeName: HMG-CoA reductase degradation protein 4
displayName UniProt:P33755 NPL4
geneName
  • NPL4
  • HRD4
  • YBR170C
  • YBR1231
identifier P33755
isSequenceChanged false
keyword
  • 3D-structure
  • Cytoplasm
  • Endoplasmic reticulum
  • Membrane
  • mRNA transport
  • Nucleus
  • Protein transport
  • Reference proteome
  • Translocation
  • Transport
modified [InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType Protein
name
  • NPL4
otherIdentifier
  • 852468
  • GO:0000837
  • GO:0000839
  • GO:0003674
  • GO:0005515
  • GO:0005575
  • GO:0005634
  • GO:0005737
  • GO:0005783
  • GO:0005789
  • GO:0006274
  • GO:0006401
  • GO:0006414
  • GO:0006511
  • GO:0006515
  • GO:0007010
  • GO:0008150
  • GO:0008270
  • GO:0015031
  • GO:0015931
  • GO:0016020
  • GO:0019899
  • GO:0030433
  • GO:0030894
  • GO:0030970
  • GO:0031625
  • GO:0031965
  • GO:0032182
  • GO:0034098
  • GO:0036266
  • GO:0036435
  • GO:0036503
  • GO:0042175
  • GO:0043130
  • GO:0043161
  • GO:0043167
  • GO:0048471
  • GO:0051028
  • GO:0051228
  • GO:0051603
  • GO:0051974
  • GO:0070651
  • GO:0071629
  • GO:0071712
  • GO:0072344
  • GO:0072665
  • GO:0072671
  • GO:0099638
  • GO:1900182
  • GO:1990112
  • GO:1990116
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • NPL4_YEAST
  • D6VQG6
sequenceLength 580
species [Species:68322] Saccharomyces cerevisiae
stId uniprot:P33755
url http://purl.uniprot.org/uniprot/P33755
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