UniProt:P33435 Mmp13

chain
  • signal peptide:1-19
  • propeptide:20-104
  • chain:105-472
checksum 67F437B89B4D0DBD
comment
  • FUNCTION Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.COFACTOR Can bind about 5 Ca(2+) ions per subunit.COFACTOR Binds 2 Zn(2+) ions per subunit.SUBCELLULAR LOCATION Detected in epidermal cells and stromal fibroblasts in wounded skin, but not in normal skin (at protein level). Detected in embryonic hypertrophic chondrocytes and newly recruited bone cells at primary ossification centers. After adult bone fracture, detected in periosteum and in chondrocytes in the cartilage. Detected in immature and mature osteoblasts in the fracture callus. Detected in calvaria from neonates. Detected in wounded skin, but not in normal skin.DOMAIN The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme (By similarity).DOMAIN The C-terminal region binds to collagen.PTM The proenzyme is activated by removal of the propeptide; this cleavage can be effected by other matrix metalloproteinases, such as MMP2, MMP3 and MMP14 and may involve several cleavage steps. Cleavage can also be autocatalytic, after partial maturation by another protease or after treatment with 4-aminophenylmercuric acetate (APMA) (in vitro) (By similarity).PTM N-glycosylated.PTM Tyrosine phosphorylated by PKDCC/VLK.DISRUPTION PHENOTYPE No visible phenotype. Mice are born at the expected Mendelian rate, are fertile and have a normal life span. Mutant embryos show a delay in the development of the primary ossification centers. Besides, they display an increased length of the growth plates of the long bones from the hind limbs (PubMed:15563592). Three week old mutant mice display an increased trabecular bone volume due to an increase in the length of the hypertrophic chondrocyte zone of the growth plate. This phenotype persists during several months (PubMed:15563592, PubMed:15539485), but one year old mutant mice display no longer any difference relative to wild-type (PubMed:15539485). After bone fractures, mutant mice show delays in carticage remodeling and resorption, as well as an increased volume of spongy bone mass. In addition, mutant mice show delayed healing of cutaneous wounds that is most evident three to seven days after wounding. The delay in wound healing and in re-epithelialization is exacerbated in mice lacking both Mmp13 and Mmp9.SIMILARITY Belongs to the peptidase M10A family.
crossReference
databaseName UniProt
dbId 95381
description
  • recommendedName: Collagenase 3 ecNumber: 3.4.24.- alternativeName: Matrix metalloproteinase-13 shortName: MMP-13
displayName UniProt:P33435 Mmp13
geneName
  • Mmp13
identifier P33435
isSequenceChanged false
keyword
  • 3D-structure
  • Calcium
  • Collagen degradation
  • Direct protein sequencing
  • Disulfide bond
  • Extracellular matrix
  • Glycoprotein
  • Hydrolase
  • Metal-binding
  • Metalloprotease
  • Phosphoprotein
  • Protease
  • Reference proteome
  • Repeat
  • Secreted
  • Signal
  • Zinc
  • Zymogen
modified [InstanceEdit:9852000] Weiser, Joel, 2023-11-03
moleculeType Protein
name
  • Mmp13
otherIdentifier
  • 100484_at
  • 10583044
  • 1417256_at
  • 17386
  • 17514482
  • 4321242
  • 4363253
  • 4399105
  • 4530145
  • 4856821
  • 4882081
  • 4928623
  • 5001549
  • 5039503
  • 5285682
  • 5490916
  • 5503372
  • A_51_P184484
  • GE34028
  • GO:0001958
  • GO:0003417
  • GO:0004175
  • GO:0004222
  • GO:0005509
  • GO:0005518
  • GO:0005576
  • GO:0005615
  • GO:0006508
  • GO:0008233
  • GO:0008237
  • GO:0008270
  • GO:0016787
  • GO:0022617
  • GO:0030198
  • GO:0030282
  • GO:0030312
  • GO:0030574
  • GO:0031012
  • GO:0046872
  • GO:0048856
  • GO:0060349
  • ILMN_1249336
  • ILMN_2737685
  • ILMN_2799267
  • X66473_s_at
  • mMC001582
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • MMP13_MOUSE
sequenceLength 472
species [Species:48892] Mus musculus
stId uniprot:P33435
url http://purl.uniprot.org/uniprot/P33435
Cite Us!