UniProt:P0CS91 SSC1

chain
  • transit peptide:1-23
  • chain:24-655
checksum 2B08AF9E3BC21082
comment
  • FUNCTION Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Constitutes the ATP-driven core of the motor and binds the precursor preprotein. Required for the import of the processed frataxin homolog YFH1 into the mitochondrion (By similarity).FUNCTION Acts as a non-catalytic component of endonuclease SceI (endo.SceI), which cleaves specifically at multiple sites on mitochondrial DNA and produces double-stranded breaks. SSC1 confers broader sequence specificity, greater stability, and higher activity on the catalytic subunit.CATALYTIC ACTIVITY ATP + H2O = ADP + phosphate + H(+)SUBUNIT Component of the PAM complex, at least composed of SSC1 (mtHsp70), MGE1, TIM44, PAM16/TIM16, PAM17 and PAM18/TIM14. In the complex, SSC1 interacts directly with PAM18 and TIM44. Interacts with NAP1 (By similarity). Component of endonuclease SceI (endo.SceI), which is a heterodimer of ENS2 and SSC1.INTERACTION Belongs to the heat shock protein 70 family.CAUTION S.cerevisiae displays strain polymorphism with regard to Endo.SceI endouclease activity. This is due to the mitochondrion-encoded, catalytic subunit ENS2, which exhibits strain polymorphism. It can be either present as continuous ORF in the mitochondrial genome (e.g. strain IAM 4274), present but disrupted by the insertion of GC clusters (e.g. strain D273-10B/A), or completely absent in the mitochondrial genome (e.g. strain S288c). Sequences for the 2 subunits ENS2 (AC P12294) and SSC1 (AC P0CS91) for an active Endo.SceI endonuclease can be found in strain IAM 4274.
created [InstanceEdit:2455454] Weiser, JD
databaseName UniProt
dbId 2455466
description
  • recommendedName: fullName evidence="8"Import motor subunit, mitochondrial ecNumber evidence="2"3.6.4.10 alternativeName: fullName evidence="8"Endonuclease SceI 75 kDa subunit shortName evidence="8"Endo.SceI 75 kDa subunit alternativeName: fullName evidence="8"Heat shock protein SSC1, mitochondrial alternativeName: fullName evidence="8"mtHSP70
displayName UniProt:P0CS91 SSC1
geneName
  • SSC1
  • ENS1
identifier P0CS91
isSequenceChanged false
keyword
  • ATP-binding
  • Chaperone
  • Direct protein sequencing
  • Hydrolase
  • Mitochondrion
  • Nucleotide-binding
  • Phosphoprotein
  • Stress response
  • Transit peptide
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
moleculeType Protein
name
  • SSC1
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • HSP77_YEASX
  • D6VWL6
  • P12398
sequenceLength 655
species [Species:68322] Saccharomyces cerevisiae
stId uniprot:P0CS91
url http://purl.uniprot.org/uniprot/P0CS91
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