UniProt:P05141 SLC25A5

chain
  • chain:1-298
  • initiator methionine:1
  • chain:2-298
checksum DC53D083E7217AFE
comment
  • FUNCTION ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (By similarity). Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity). In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (By similarity). Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (By similarity). Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A5/ANT2 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it (By similarity). Probably mediates mitochondrial uncoupling in tissues that do not express UCP1 (By similarity). Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (PubMed:31883789). It is however unclear if SLC25A5/ANT2 constitutes a pore-forming component of mPTP or regulates it (By similarity). Acts as a regulator of mitophagy independently of ADP:ATP antiporter activity: promotes mitophagy via interaction with TIMM44, leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1 (By similarity). As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed:20797633).CATALYTIC ACTIVITY ADP(in) + ATP(out) = ADP(out) + ATP(in)CATALYTIC ACTIVITY H(+)(in) = H(+)(out)ACTIVITY REGULATION The matrix-open state (m-state) is inhibited by the membrane-permeable bongkrekic acid (BKA). The cytoplasmic-open state (c-state) is inhibited by the membrane-impermeable toxic inhibitor carboxyatractyloside (CATR) (By similarity). Proton transporter activity is inhibited by ADP:ATP antiporter activity (By similarity).SUBUNIT Monomer (By similarity). Component of the MMXD complex, which includes CIAO1, ERCC2, CIAO2B, MMS19 and SLC25A5/ANT2 (PubMed:20797633). Interacts with AK4 (PubMed:19130895). Interacts with ARHGAP11B, thereby inhibiting the mitochondrial permeability transition pore (mPTP) (PubMed:31883789). Interacts with TIMM44; leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1 (By similarity).SUBUNIT (Microbial infection) Interacts with HIV-1 Vpr.INTERACTION May localize to non-mitochondrial membranes.TISSUE SPECIFICITY Expressed in erythrocytes (at protein level).DOMAIN The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue.PTM Trimethylated by ANTKMT at Lys-52.SIMILARITY Belongs to the mitochondrial carrier (TC 2.A.29) family.
crossReference
databaseName UniProt
dbId 49744
description
  • recommendedName: fullName evidence="23"ADP/ATP translocase 2 alternativeName: fullName evidence="21"ADP,ATP carrier protein 2 alternativeName: fullName evidence="20"ADP,ATP carrier protein, fibroblast isoform alternativeName: fullName evidence="22"Adenine nucleotide translocator 2 shortName: ANT 2 alternativeName: fullName evidence="23"Solute carrier family 25 member 5 component recommendedName: ADP/ATP translocase 2, N-terminally processed /component
displayName UniProt:P05141 SLC25A5
geneName
  • SLC25A5
  • AAC2
  • ANT2
identifier P05141
isSequenceChanged false
keyword
  • Acetylation
  • Antiport
  • ATP-binding
  • Chromosome partition
  • Direct protein sequencing
  • Host-virus interaction
  • Membrane
  • Methylation
  • Mitochondrion
  • Mitochondrion inner membrane
  • Nucleotide-binding
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • Repeat
  • Transmembrane
  • Transmembrane helix
  • Transport
modified [InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType Protein
name
  • SLC25A5
otherIdentifier
  • 11715399_at
  • 11715400_x_at
  • 17106574
  • 200657_PM_at
  • 200657_at
  • 2547789
  • 292
  • 2996022
  • 3002414
  • 37740_r_at
  • 3988831
  • 3988832
  • 3988836
  • 3988837
  • 3988838
  • 3988839
  • 3988840
  • 3988841
  • 3988842
  • 3988843
  • 3988844
  • 3988845
  • 3988846
  • 3988847
  • 3988848
  • 8169640
  • A_14_P120807
  • A_23_P137057
  • A_23_P436179
  • A_24_P182182
  • A_24_P182183
  • GE57465
  • GO:0000295
  • GO:0002376
  • GO:0003723
  • GO:0005471
  • GO:0005515
  • GO:0005634
  • GO:0005739
  • GO:0005743
  • GO:0005757
  • GO:0005886
  • GO:0007059
  • GO:0008284
  • GO:0015078
  • GO:0015207
  • GO:0015297
  • GO:0015853
  • GO:0016020
  • GO:0017077
  • GO:0030154
  • GO:0030183
  • GO:0030218
  • GO:0031625
  • GO:0042645
  • GO:0046902
  • GO:0051503
  • GO:0055085
  • GO:0071817
  • GO:0140021
  • GO:1901029
  • GO:1901526
  • GO:1902600
  • GO:1990544
  • GO:1990830
  • GO:1990845
  • HMNXSV003004676
  • ILMN_1774062
  • J02683_s_at
  • TC0X000586.hg
  • g4502098_3p_at
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • ADT2_HUMAN
  • B2RCV1
  • O43350
sequenceLength 298
species [Species:48887] Homo sapiens
stId uniprot:P05141
url http://purl.uniprot.org/uniprot/P05141
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