UniProt:P00741 F9

chain
  • signal peptide:1-25
  • chain:26-462
  • propeptide:29-46
  • chain:47-192
  • propeptide:193-227
  • chain:228-462
checksum 415B2A7BD6EA256A
comment
  • FUNCTION Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.CATALYTIC ACTIVITY Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.SUBUNIT Heterodimer of a light chain and a heavy chain; disulfide-linked (By similarity). Interacts (inactive and activated) with F11 (activated) in calcium-dependent manner (By similarity). Interacts with SERPINC1 (By similarity). Interacts with the heterodimeric snake venom coagulation factor IX-binding protein (PubMed:12695512).SUBCELLULAR LOCATION Detected in blood plasma (at protein level) (PubMed:12695512).DOMAIN Calcium binds to the gamma-carboxyglutamic acid (Gla) residues in the Gla domain (PubMed:12695512). Calcium can also bind, with stronger affinity, to another site beyond the Gla domain (By similarity). Under physiological ion concentrations, Ca(2+) is displaced by Mg(2+) from some of the gammaglutamate residues in the N-terminal Gla domain. This leads to a subtle conformation change that may affect the interaction with its binding protein (PubMed:12695512).PTM Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease (By similarity). Activated by coagulation factor VIIa-tissue factor (F7-F3) complex in calcium-dependent manner (By similarity).PTM The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.PTM Predominantly O-glucosylated at Ser-99 by POGLUT1 in vitro.PTM Carboxylated by vitamin K-dependent GGCX to form gamma-carboxyglutamate; these residues are essential for the binding of calcium.SIMILARITY Belongs to the peptidase S1 family.
crossReference
databaseName UniProt
dbId 90198
description
  • recommendedName: Coagulation factor IX ecNumber: 3.4.21.22 alternativeName: Christmas factor component recommendedName: Coagulation factor IXa light chain /component component recommendedName: Coagulation factor IXa heavy chain /component
displayName UniProt:P00741 F9
geneName
  • F9
identifier P00741
isSequenceChanged false
keyword
  • 3D-structure
  • Blood coagulation
  • Calcium
  • Direct protein sequencing
  • Disulfide bond
  • EGF-like domain
  • Gamma-carboxyglutamic acid
  • Glycoprotein
  • Hemophilia
  • Hemostasis
  • Hydrolase
  • Hydroxylation
  • Magnesium
  • Metal-binding
  • Phosphoprotein
  • Protease
  • Reference proteome
  • Secreted
  • Serine protease
  • Signal
  • Sulfation
  • Zymogen
modified [InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType Protein
name
  • F9
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • FA9_BOVIN
  • F1MFL4
sequenceLength 462
species [Species:48898] Bos taurus
stId uniprot:P00741
url http://purl.uniprot.org/uniprot/P00741
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