Reactome | UniProt:P00491 PNP

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UniProt:P00491 PNP Show undefined attributes

chain	chain:1-289
checksum	91622FB1D26479D3
comment	FUNCTION Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate (PubMed:23438750, PubMed:3029074, PubMed:9305964). Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine (PubMed:9305964).CATALYTIC ACTIVITY inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthineCATALYTIC ACTIVITY guanosine + phosphate = alpha-D-ribose 1-phosphate + guanineCATALYTIC ACTIVITY 2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanineCATALYTIC ACTIVITY 2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthineACTIVITY REGULATION Inhibited by 5'-deaza-1'-aza-2c-deoxy-1'-(9-methylene)-Immucilin-G (DADMe-ImmG).BIOPHYSICOCHEMICAL PROPERTIES kcat is 57 sec(-1) with inosine as substrate (PubMed:9305964). kcat is 47 sec(-1) with inosine as substrate (PubMed:23438750). kcat is 70 sec(-1) with hypoxanthine as substrate (PubMed:9305964). kcat is 28 sec(-1) with guanosine as substrate (PubMed:9305964). kcat is 48 sec(-1) with guanine as substrate (PubMed:9305964). kcat is 0.0024 sec(-1) with adenosine as substrate (PubMed:9305964). kcat is 0.31 sec(-1) with adenine as substrate (PubMed:9305964).PATHWAY Purine metabolism; purine nucleoside salvage.SUBUNIT Homotrimer.INTERACTION Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the PNP/MTAP phosphorylase family.ONLINE INFORMATION NP mutation db
crossReference	[DatabaseIdentifier:12188875] PDB:1V41 [DatabaseIdentifier:12817488] ZINC target:P00491 [DatabaseIdentifier:12814054] ZINC - Substances:PNPH_HUMAN [DatabaseIdentifier:12810631] ZINC - Predictions - Purchasable:PNPH_HUMAN [DatabaseIdentifier:12807740] ZINC - Metabolites:PNPH_HUMAN [DatabaseIdentifier:12807086] ZINC - Investigational:PNPH_HUMAN [DatabaseIdentifier:12804930] ZINC - Biogenic:PNPH_HUMAN [DatabaseIdentifier:12325536] RefSeq:NP_000261.2 [DatabaseIdentifier:12286992] Pharos - Targets:P00491 [DatabaseIdentifier:12247063] PRO:P00491 [DatabaseIdentifier:12188897] PDB:3GGS [DatabaseIdentifier:12188898] PDB:2OC4 [DatabaseIdentifier:12188885] PDB:1PF7 [DatabaseIdentifier:12188863] PDB:7ZSN [DatabaseIdentifier:12188884] PDB:1RCT [DatabaseIdentifier:12188899] PDB:7ZSP [DatabaseIdentifier:12188881] PDB:2A0X [DatabaseIdentifier:12188887] PDB:1ULB [DatabaseIdentifier:12188871] PDB:1V45 [DatabaseIdentifier:12188861] PDB:2Q7O [DatabaseIdentifier:12188886] PDB:4ECE [DatabaseIdentifier:12188877] PDB:1YRY [DatabaseIdentifier:12188890] PDB:2A0Y [DatabaseIdentifier:12188868] PDB:3K8Q [DatabaseIdentifier:12188900] PDB:1RSZ [DatabaseIdentifier:12188873] PDB:1V3Q [DatabaseIdentifier:12188862] PDB:3BGS [DatabaseIdentifier:12188865] PDB:5UGF [DatabaseIdentifier:12188893] PDB:4GKA [DatabaseIdentifier:12188882] PDB:2A0W [DatabaseIdentifier:12188895] PDB:3GB9 [DatabaseIdentifier:12188880] PDB:4EB8 [DatabaseIdentifier:12188883] PDB:2OC9 [DatabaseIdentifier:12188869] PDB:1V2H [DatabaseIdentifier:12188866] PDB:7ZSL [DatabaseIdentifier:12188896] PDB:1PWY [DatabaseIdentifier:12188867] PDB:7ZSM [DatabaseIdentifier:12188864] PDB:7ZSO [DatabaseIdentifier:12188889] PDB:3D1V [DatabaseIdentifier:12188879] PDB:4EAR [DatabaseIdentifier:12188891] PDB:3PHB [DatabaseIdentifier:12188874] PDB:1RFG [DatabaseIdentifier:12188870] PDB:1M73 [DatabaseIdentifier:12188894] PDB:1RR6 [DatabaseIdentifier:12188901] PDB:5ETJ [DatabaseIdentifier:12188876] PDB:3K8O [DatabaseIdentifier:12188888] PDB:1ULA [DatabaseIdentifier:11452876] ENSEMBL:ENSG00000198805 [DatabaseIdentifier:11452877] ENSEMBL:ENSG00000291814 [DatabaseIdentifier:11542659] ENSEMBL:ENSP00000517386 [DatabaseIdentifier:11542660] ENSEMBL:ENSP00000354532 [DatabaseIdentifier:11542662] ENSEMBL:ENSP00000517384 [DatabaseIdentifier:11542661] ENSEMBL:ENSP00000513359 [DatabaseIdentifier:11665361] ENSEMBL:ENST00000708853 [DatabaseIdentifier:11665359] ENSEMBL:ENST00000697613 [DatabaseIdentifier:11665360] ENSEMBL:ENST00000708857 [DatabaseIdentifier:11665358] ENSEMBL:ENST00000361505 [DatabaseIdentifier:11781073] Guide to Pharmacology - Targets:2841 [DatabaseIdentifier:11789119] GeneCards:PNP [DatabaseIdentifier:11804747] GlyGen:P00491 [DatabaseIdentifier:11834236] HMDB Protein:HMDBP03752 [DatabaseIdentifier:11842143] HPA:ENSG00000198805-PNP [DatabaseIdentifier:12126738] OpenTargets:ENSG00000198805 [DatabaseIdentifier:12137986] Orphanet:PNP [DatabaseIdentifier:12188872] PDB:2ON6 [DatabaseIdentifier:12188878] PDB:3INY [DatabaseIdentifier:12188892] PDB:1RT9
databaseName	UniProt
dbId	61934
description	recommendedName: Purine nucleoside phosphorylase shortName: PNP ecNumber evidence="8 12"2.4.2.1 alternativeName: Inosine phosphorylase alternativeName: Inosine-guanosine phosphorylase
displayName	UniProt:P00491 PNP
geneName	PNP NP
identifier	P00491
isSequenceChanged	false
keyword	3D-structure Acetylation Cytoplasm Direct protein sequencing Disease variant Glycosyltransferase Phosphoprotein Proteomics identification Purine salvage Reference proteome Transferase
modified	[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType	Protein
name	PNP
otherIdentifier	11718400_a_at 16781536 201695_PM_s_at 201695_s_at 3527515 3527516 3527517 3527518 3527519 3527520 3527521 3527522 3527523 3527524 3527525 3527527 3527528 3527529 3527530 3527531 430_at 4860 7973067 A_14_P105467 A_23_P140256 GO:0000255 GO:0001882 GO:0002060 GO:0002376 GO:0003824 GO:0004731 GO:0005515 GO:0005576 GO:0005737 GO:0005829 GO:0006139 GO:0006148 GO:0006149 GO:0006157 GO:0006166 GO:0006204 GO:0006738 GO:0006955 GO:0009116 GO:0009165 GO:0009410 GO:0016740 GO:0016757 GO:0016763 GO:0032743 GO:0034418 GO:0034774 GO:0042102 GO:0042301 GO:0042802 GO:0043101 GO:0046059 GO:0046638 GO:0047975 GO:0055086 GO:0070062 GO:1901135 GO:1904813 HMNXSV003001137 ILMN_2172174 K02574_at PH_hs_0004336 TC14000056.hg TC14001578.hg g4557800_3p_a_at
physicalEntity	[EntityWithAccessionedSequence:R-HSA-61933] PNP [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-6803278] PNP [ficolin-1-rich granule lumen] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-6806505] PNP [extracellular region] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-6803318] PNP [secretory granule lumen] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-9735785] PNP D128G [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-9735781] PNP E89K [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-9735772] PNP R234P [cytosol] - Homo sapiens
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:ensembl:ENSG00000198805] ENSEMBL:ENSG00000198805 PNP [ReferenceDNASequence:12726617] UCSC:P00491 PNP [ReferenceDNASequence:11318025] BioGPS Gene:4860 PNP [ReferenceDNASequence:11397956] COSMIC - genes:PNP PNP [ReferenceDNASequence:11415656] CTD Gene:4860 PNP [ReferenceDNASequence:11434720] dbSNP Gene:4860 PNP [ReferenceDNASequence:11820771] HGNC:7892 PNP [ReferenceDNASequence:11861155] KEGG:hsa:4860 PNP [ReferenceDNASequence:11947814] Monarch:4860 PNP [ReferenceDNASequence:12031907] NCBI Gene:4860 PNP [ReferenceDNASequence:12111695] OMIM:164050 PNP
referenceTranscript	[ReferenceRNASequence:12507473] RefSeq:NM_000270.4 PNP
schemaClass	ReferenceGeneProduct
secondaryIdentifier	PNPH_HUMAN B2R8S5 D3DS00 Q15160 Q5PZ03
sequenceLength	289
species	[Species:48887] Homo sapiens
stId	uniprot:P00491
url	http://purl.uniprot.org/uniprot/P00491

Referrals

(referenceEntity)	[EntityWithAccessionedSequence:R-HSA-61933] PNP [cytosol] [EntityWithAccessionedSequence:R-HSA-6803278] PNP [ficolin-1-rich granule lumen] [EntityWithAccessionedSequence:R-HSA-6806505] PNP [extracellular region] [EntityWithAccessionedSequence:R-HSA-6803318] PNP [secretory granule lumen] [EntityWithAccessionedSequence:R-HSA-9735785] PNP D128G [cytosol] [EntityWithAccessionedSequence:R-HSA-9735781] PNP E89K [cytosol] [EntityWithAccessionedSequence:R-HSA-9735772] PNP R234P [cytosol]
(referenceSequence)	[ReplacedResidue:9735777] L-aspartic acid 128 replaced with glycine [ReplacedResidue:9735774] L-glutamic acid 89 replaced with L-lysine [ReplacedResidue:9735779] L-arginine 234 replaced with L-proline
(interactor)	[UndirectedInteraction:11146993] UniProt:P17612 <-> UniProt:P00491 (IntAct) [UndirectedInteraction:11204123] UniProt:P63000 <-> UniProt:P00491 (IntAct) [UndirectedInteraction:11214267] UniProt:P06241 <-> UniProt:P00491 (IntAct) [UndirectedInteraction:11239473] UniProt:Q92673 <-> UniProt:P00491 (IntAct) [UndirectedInteraction:11258651] UniProt:Q9BXM7 <-> UniProt:P00491 (IntAct) [UndirectedInteraction:11269959] UniProt:P14136 <-> UniProt:P00491 (IntAct) [UndirectedInteraction:11274033] UniProt:P05067 <-> UniProt:P00491 (IntAct)

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