| chain |
|
| checksum |
D204D8B91CFF00A4
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| comment |
-
FUNCTION Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis (By similarity). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly (By similarity). Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis (By similarity). Required for central/midzone spindle assembly and cleavage furrow formation (By similarity). Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (By similarity). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP (By similarity). Phosphorylation of INCENP leads to increased AURKB activity (By similarity). Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/vimentin, HASPIN, and histone H3 (By similarity). A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres (By similarity). Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively) (PubMed:11784863). AURKB is also required for kinetochore localization of BUB1 and SGO1 (By similarity). Phosphorylation of p53/TP53 negatively regulates its transcriptional activity (By similarity). Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes (PubMed:24034696). Acts as an inhibitor of CGAS during mitosis: catalyzes phosphorylation of the N-terminus of CGAS during the G2-M transition, blocking CGAS liquid phase separation and activation, and thereby preventing CGAS-induced autoimmunity (By similarity). Phosphorylates KRT5 during anaphase and telophase (PubMed:29518391). Phosphorylates ATXN10 which promotes phosphorylation of ATXN10 by PLK1 and may play a role in the regulation of cytokinesis and stimulating the proteasomal degradation of ATXN10 (By similarity).CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)ACTIVITY REGULATION Activity is greatly increased when AURKB is within the CPC complex. In particular, AURKB-phosphorylated INCENP acts as an activator of AURKB. Positive feedback between HASPIN and AURKB contributes to CPC localization.SUBUNIT Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; predominantly independent AURKB- and AURKC-containing complexes exist. Associates with RACGAP1 during M phase. Interacts with SPDYC; this interaction may be required for proper localization of active, Thr-237-phosphorylated AURKB form during prometaphase and metaphase. Interacts with p53/TP53. Interacts (via the middle kinase domain) with NOC2L (via the N- and C-terminus domains). Interacts with CDCA1. Interacts with EVI5. Interacts with JTB. Interacts with NDC80. Interacts with PSMA3 (By similarity). Interacts with RNF2/RING1B (PubMed:24034696). Interacts with SEPTIN1. Interacts with SIRT2. Interacts with TACC1. Interacts with TTC28 (By similarity).SUBCELLULAR LOCATION Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the midbody. Proper localization of the active, Thr-237-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Colocalized with SIRT2 during cytokinesis with the midbody. Localization (and probably targeting of the CPC) to the inner centromere occurs predominantly in regions with overlapping mitosis-specific histone phosphorylations H3pT3 and H2ApT12.TISSUE SPECIFICITY Expressed in testis, intestine and spleen. All of them are tissues that contain a large number of proliferating cells. Expressed during S phase, in a cell-cycle-dependent fashion.DEVELOPMENTAL STAGE Strongly expressed in 8.5 and 12.5 dpc.PTM The phosphorylation of Thr-237 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-237 phosphorylation is indispensable for the AURKB kinase activity.PTM Acetylated at Lys-220 by KAT5 at kinetochores, increasing AURKB activity and promoting accurate chromosome segregation in mitosis.PTM Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. Deubiquitinated by USP35; inhibiting CDH1-mediated degradation of AURKB.SIMILARITY Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
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| crossReference |
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| databaseName |
UniProt
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| dbId |
85700
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| description |
-
recommendedName: fullName evidence="9"Aurora kinase B ecNumber evidence="6"2.7.11.1 alternativeName: Aurora 1 alternativeName: Aurora- and IPL1-like midbody-associated protein 1 alternativeName: fullName evidence="11"Aurora/IPL1-related kinase 2 shortName evidence="11"ARK-2 shortName: Aurora-related kinase 2 alternativeName: fullName evidence="10"STK-1 alternativeName: Serine/threonine-protein kinase 12 alternativeName: Serine/threonine-protein kinase 5 alternativeName: fullName evidence="12"Serine/threonine-protein kinase aurora-B
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| displayName |
UniProt:O70126 Aurkb
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| geneName |
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Aurkb
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Aik2
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Aim1
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Airk2
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Ark2
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Stk1
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Stk12
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Stk5
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| identifier |
O70126
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| isSequenceChanged |
false
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| keyword |
-
Acetylation
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ATP-binding
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Cell cycle
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Cell division
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Centromere
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Chromosome
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Cytoplasm
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Cytoskeleton
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Kinase
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Kinetochore
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Mitosis
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Nucleotide-binding
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Nucleus
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Phosphoprotein
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Reference proteome
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Serine/threonine-protein kinase
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Transferase
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Ubl conjugation
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| modified |
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
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| moleculeType |
Protein
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| name |
|
| otherIdentifier |
-
10377405
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1424128_x_at
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1451246_s_at
-
17251485
-
20877
-
4329527
-
4416371
-
4432111
-
4478480
-
4514316
-
4551966
-
4561548
-
4610570
-
4660779
-
4676038
-
4780508
-
4795195
-
4878198
-
4956559
-
4977431
-
5049633
-
5162529
-
5427825
-
5522616
-
5523784
-
5547921
-
5556883
-
5603078
-
5606770
-
98469_at
-
A_51_P415059
-
A_55_P1980631
-
A_55_P2884008
-
A_66_P103201
-
D21099_s_at
-
GE40529
-
GO:0000122
-
GO:0000166
-
GO:0000278
-
GO:0000281
-
GO:0000775
-
GO:0000776
-
GO:0000779
-
GO:0000910
-
GO:0000922
-
GO:0002376
-
GO:0002903
-
GO:0003824
-
GO:0004672
-
GO:0004674
-
GO:0005515
-
GO:0005524
-
GO:0005634
-
GO:0005654
-
GO:0005694
-
GO:0005737
-
GO:0005813
-
GO:0005815
-
GO:0005819
-
GO:0005856
-
GO:0005876
-
GO:0006325
-
GO:0006338
-
GO:0006355
-
GO:0006468
-
GO:0007010
-
GO:0007051
-
GO:0007052
-
GO:0010369
-
GO:0015630
-
GO:0016301
-
GO:0016740
-
GO:0019900
-
GO:0030496
-
GO:0031117
-
GO:0032133
-
GO:0032206
-
GO:0032465
-
GO:0032466
-
GO:0032467
-
GO:0034501
-
GO:0034644
-
GO:0036089
-
GO:0043226
-
GO:0043687
-
GO:0044022
-
GO:0044839
-
GO:0044878
-
GO:0045824
-
GO:0051233
-
GO:0051256
-
GO:0051301
-
GO:0051315
-
GO:0061024
-
GO:0061952
-
GO:0062033
-
GO:0090267
-
GO:0097431
-
GO:0106310
-
GO:0140096
-
GO:0140273
-
GO:0140896
-
GO:0160049
-
GO:1901970
-
GO:1902425
-
GO:1903490
-
GO:1905116
-
GO:1990023
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GO:1990758
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ILMN_1239230
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mMC000969
|
| physicalEntity |
|
| referenceDatabase |
[ReferenceDatabase:2] UniProt
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| referenceGene |
|
| referenceTranscript |
|
| schemaClass |
ReferenceGeneProduct
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| secondaryIdentifier |
-
AURKB_MOUSE
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Q61882
-
Q8C6C1
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| sequenceLength |
345
|
| species |
[Species:48892] Mus musculus
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| stId |
uniprot:O70126
|
| url |
http://purl.uniprot.org/uniprot/O70126
|
