UniProt:O60678 PRMT3

chain
  • initiator methionine:1
  • chain:2-531
checksum 71EC59E2BEDBE7AC
comment
  • FUNCTION Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (PubMed:22795084, PubMed:23445220, PubMed:25728001, PubMed:31378783, PubMed:33495566, PubMed:39513743). Catalyzes the asymmetric arginine dimethylation at multiple sites in the Arg/Gly-rich region of small ribosomal subunit protein uS5/RPS2 (PubMed:22795084). Also appears to methylate other ribosomal proteins (By similarity). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity (PubMed:33495566). Contributes to methylation of histone H4 'Arg-3', a specific tag for epigenetic transcriptional activation (PubMed:25728001, PubMed:31378783, PubMed:39513743). Mediates asymmetric arginine dimethylation of histone H4 'Arg-3' (H4R3me2a) in the promoter region of miRNA miR-3648, to promote its transcription and osteogenesis (PubMed:31378783).CATALYTIC ACTIVITY L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)ACTIVITY REGULATION Inhibited by N-ethylmaleimide and high concentrations of zinc chloride (By similarity). Allosterically inhibited by SGC707 (PubMed:25728001). Allosterically inhibited by (1-(benzo[d][1,2,3]thiadiazol- 6-yl)-3-(2-cyclohexenylethyl)urea) and derivatives thereof (PubMed:22795084, PubMed:23445220).BIOPHYSICOCHEMICAL PROPERTIES kcat is 0.1 min(-1) with RPS2 as substrate (at pH 8).SUBUNIT Monomer and homodimer (By similarity). Interacts with EPB41L3 (via FERM domain); the interaction is direct and inhibits the protein-arginine N-methyltransferase activity of PRMT3 (PubMed:15334060). Interacts with the 40S ribosomal protein RPS2/uS5 (PubMed:21059412, PubMed:27697862, PubMed:30530495). Interacts with ALDH1A1; the interaction is direct, inhibits ALDH1A1 aldehyde dehydrogenase activity and is independent of the methyltransferase activity of PRMT3 (PubMed:33495566). Interacts (via zinc-finger) with ZNF200 (via C-terminus); the interaction is direct and required to localize PRMT3 to the nucleus and inhibit its proteasomal degradation (PubMed:39513743).INTERACTION Localized to the nucleus by ZNF200.ALTERNATIVE PRODUCTS Induced during osteogenic differentiation of mesenchymal stem cells (at protein level).DOMAIN The C2H2-type zinc-finger is responsible for substrate specificity.SIMILARITY Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
crossReference
databaseName UniProt
dbId 50008
description
  • recommendedName: fullName evidence="22"Protein arginine N-methyltransferase 3 ecNumber evidence="19 20 21 22 23"2.1.1.319 alternativeName: fullName evidence="24"Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
displayName UniProt:O60678 PRMT3
geneName
  • PRMT3
  • HRMT1L3
identifier O60678
isSequenceChanged false
keyword
  • 3D-structure
  • Acetylation
  • Alternative splicing
  • Cytoplasm
  • Metal-binding
  • Methyltransferase
  • Nucleus
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • S-adenosyl-L-methionine
  • Transferase
  • Zinc
  • Zinc-finger
modified [InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType Protein
name
  • PRMT3
otherIdentifier
  • 10196
  • 11726759_s_at
  • 11740719_a_at
  • 11742086_a_at
  • 16722799
  • 213320_PM_at
  • 213320_at
  • 3323444
  • 3323445
  • 3323446
  • 3323447
  • 3323448
  • 3323449
  • 3323451
  • 3323452
  • 3323453
  • 3323454
  • 3323455
  • 3323456
  • 3323457
  • 3323458
  • 3323459
  • 3323460
  • 3323461
  • 3323462
  • 3323463
  • 3323465
  • 3323466
  • 3323468
  • 3323477
  • 3323478
  • 3323483
  • 3323484
  • 3323485
  • 3323490
  • 34998_at
  • 7938890
  • A_14_P120138
  • A_23_P9894
  • GE486562
  • GO:0003824
  • GO:0005515
  • GO:0005634
  • GO:0005737
  • GO:0005829
  • GO:0006325
  • GO:0006338
  • GO:0006355
  • GO:0008168
  • GO:0008270
  • GO:0016020
  • GO:0016274
  • GO:0016740
  • GO:0018216
  • GO:0031397
  • GO:0032259
  • GO:0035241
  • GO:0035242
  • GO:0042054
  • GO:0044020
  • GO:0045669
  • GO:0045815
  • GO:0046872
  • GO:0140096
  • GO:1900053
  • HMNXSV003022737
  • Hs.152337.0.S1_3p_at
  • ILMN_1662964
  • ILMN_1819790
  • PH_hs_0009167
  • TC11000257.hg
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • ANM3_HUMAN
  • A0A0A0MSN7
  • B4DUC7
sequenceLength 531
species [Species:48887] Homo sapiens
stId uniprot:O60678
url http://purl.uniprot.org/uniprot/O60678
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