UniProt:O43809 NUDT21

chain
  • initiator methionine:1
  • chain:2-227
checksum D204243E57F1CCC5
comment
  • FUNCTION Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:14690600, PubMed:15937220, PubMed:17024186, PubMed:17098938, PubMed:29276085, PubMed:8626397, PubMed:9659921). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:14690600, PubMed:17024186, PubMed:8626397, PubMed:9659921). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:17098938, PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:17098938, PubMed:20695905, PubMed:29276085). NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7 (PubMed:29276085). Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing (PubMed:14690600, PubMed:15169763, PubMed:17024186, PubMed:20479262, PubMed:22813749, PubMed:8626397). The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (PubMed:20479262, PubMed:21295486). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (By similarity). Binds to chromatin (By similarity). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides (PubMed:18445629).SUBUNIT Homodimer (via N- and C-terminus); binds RNA as homodimer (PubMed:18445629, PubMed:20479262, PubMed:20695905). Component of the cleavage factor Im (CFIm) complex which is a heterotetramer composed of two subunits of NUDT21/CPSF5 and two subunits of CPSF6 or CPSF7 or a heterodimer of CPSF6 and CPSF7 (PubMed:14561889, PubMed:20695905, PubMed:21295486, PubMed:23187700, PubMed:8626397, PubMed:9659921). The cleavage factor Im (CFIm) complex associates with the CPSF and CSTF complexes to promote the assembly of the core mRNA 3'-processing machinery (PubMed:29276085). Interacts with CPSF6 (via the RRM domain); this interaction is direct and enhances binding to RNA (PubMed:14561889, PubMed:15169763, PubMed:17172643, PubMed:19864460, PubMed:29276085). Interacts with CPSF7 (PubMed:29276085, Ref.30). Interacts with FIP1L1; this interaction occurs in a RNA sequence-specific manner (PubMed:15937220). Interacts with PABPN1 (PubMed:15169763). Interacts (via N-terminus) with PAPOLA (via C-terminus); this interaction is direct and diminished by acetylation (PubMed:15169763, PubMed:17172643). Interacts with SNRNP70 (PubMed:14561889). Interacts with VIRMA (PubMed:29507755).INTERACTION Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460). In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles (PubMed:15169763).TISSUE SPECIFICITY Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.PTM Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 and SIRT2.SIMILARITY Belongs to the Nudix hydrolase family. CPSF5 subfamily.CAUTION Lacks the conserved metal-binding residues in the NUDIX motif and does not have hydrolase activity.
crossReference
databaseName UniProt
dbId 72009
description
  • recommendedName: fullName evidence="23"Cleavage and polyadenylation specificity factor subunit 5 alternativeName: Cleavage and polyadenylation specificity factor 25 kDa subunit shortName: CPSF 25 kDa subunit alternativeName: fullName evidence="24"Cleavage factor Im complex 25 kDa subunit shortName evidence="24"CFIm25 alternativeName: Nucleoside diphosphate-linked moiety X motif 21 shortName: Nudix motif 21 alternativeName: fullName evidence="25"Nudix hydrolase 21 alternativeName: Pre-mRNA cleavage factor Im 68 kDa subunit
displayName UniProt:O43809 NUDT21
geneName
  • NUDT21
  • CFIM25
  • CPSF25
  • CPSF5
identifier O43809
isSequenceChanged false
keyword
  • 3D-structure
  • Acetylation
  • Cytoplasm
  • Differentiation
  • Direct protein sequencing
  • Methylation
  • mRNA processing
  • Nucleus
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • RNA-binding
modified [InstanceEdit:9963647] Weiser, Joel, 2025-08-15
moleculeType Protein
name
  • NUDT21
otherIdentifier
  • 11051
  • 11718401_a_at
  • 11718402_a_at
  • 11756899_a_at
  • 16826708
  • 202697_PM_at
  • 202697_at
  • 213461_3p_at
  • 213461_PM_at
  • 213461_at
  • 224830_PM_at
  • 224830_at
  • 3692894
  • 3692896
  • 3692897
  • 3692898
  • 3692899
  • 3692900
  • 3692901
  • 3692902
  • 3692903
  • 3692904
  • 3692905
  • 3692908
  • 3692909
  • 3692912
  • 3692913
  • 3692914
  • 3692915
  • 3692916
  • 3692917
  • 3692918
  • 3692919
  • 3692920
  • 39142_at
  • 4048119
  • 64044_at
  • 8001496
  • 80880_at
  • 90992_at
  • A_23_P129614
  • A_24_P287756
  • A_33_P3418942
  • GE55148
  • GE86161
  • GO:0003682
  • GO:0003723
  • GO:0003729
  • GO:0005515
  • GO:0005634
  • GO:0005654
  • GO:0005737
  • GO:0005813
  • GO:0005815
  • GO:0005847
  • GO:0005849
  • GO:0006397
  • GO:0010608
  • GO:0016071
  • GO:0016604
  • GO:0030154
  • GO:0031124
  • GO:0034451
  • GO:0035925
  • GO:0042382
  • GO:0042802
  • GO:0042803
  • GO:0042826
  • GO:0051262
  • GO:0051290
  • GO:0065003
  • GO:0110104
  • GO:0180010
  • GO:2000738
  • GO:2000975
  • HMNXSV003003268
  • Hs.288061.1.S1_3p_at
  • Hs.288061.1.S1_3p_s_at
  • Hs.288061.1.S1_3p_x_at
  • Hs.301528.1.A1_3p_at
  • ILMN_1798886
  • ILMN_2133146
  • PH_hs_0026091
  • TC16001133.hg
  • g5901925_3p_at
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • CPSF5_HUMAN
  • Q6IB85
  • Q6NE84
sequenceLength 227
species [Species:48887] Homo sapiens
stId uniprot:O43809
url http://purl.uniprot.org/uniprot/O43809

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