After formation of the pBAF core trimer consisting of a dime...

created [InstanceEdit:9932445] Rothfels, Karen, 2024-12-19
dbId 9932438
displayName After formation of the pBAF core trimer consisting of a dime...
modified [InstanceEdit:9938986] Rothfels, Karen, 2025-02-21
schemaClass Summation
text After formation of the pBAF core trimer consisting of a dimer of SMARCC1/2 and one copy of SMARCD1/2/3, SMARCE1 and SMARCB1 are recruited (Mashtalir et al, 2018). Crosslinking and knockout studies indicate that the SMARCE1 subunit of the pBAF complex is directly recruited through interaction with the SMARCC dimer (Mashtalir et al, 2018; Mashtalir et al, 2020). Together, the SMARCC homodimer and SMARCD, SMARCE1 and SMARCB1 form the pBAF core module that lies adjacent to the nucleosome (Nakayama et al, 2017; Mashtalir et al, 2018; Mashtalir et al, 2020; He et al, 2020). Knockdown of SMARCE1 or SMARCB1 destabilize pBAF complex assembly, with SMARCE1 knockdown causing an increase in free ARID2 as assessed by gradient sedimentation. SMARCE1 and SMARCB1 are dispensable for the assembly of the ncBAF complex (Mashtalir et al, 2018).
SMARCB1 has a positive C-terminal alpha-helix that mediates contacts with acidic patches on the nucleosome (He et al, 2020; Mashtalir et al, 2020). Mutations in SMARCB1, common in pediatric rhabdoid tumors, affect the targeting of the SWI/SNF complex to enhancers such that recruitment at "super-enhancers" is maintained at the expense of regular, non-super-enhancer regions (Wang et al, 2017; Nakayama et al, 2017).
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