53BP1-deficient DT40 chicken cells exhibit elevated sensitiv...

created [InstanceEdit:351443] Saxena, A, 2008-05-22 20:44:57
dbId 351437
displayName 53BP1-deficient DT40 chicken cells exhibit elevated sensitiv...
modified [InstanceEdit:391641] Saxena, A, 2009-02-23 15:55:32
schemaClass Summation
text 53BP1-deficient DT40 chicken cells exhibit elevated sensitivity to IR-induced damage with persistent gamma-H2AX foci, indicating a role for 53BP1 in checkpoint signaling and/or DNA repair pathways. Epistasis analysis reveals that 53BP1 plays a role in a pathway distinct from the Ku- and Artemis-dependent NHEJ pathways, but still requiring DNA ligase IV. The 53BP1-dependent repair pathway is important for survival of cells irradiated with IR during the G1 phase of the cell cycle. Although intra S and G2/M checkpoints were intact, the frequency of isochromatid-type chromosomal aberrations was elevated after irradiation in 53BP1-deficient cells.
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