SMARCA4, one of two ATPase components of SWI/SNF chromatin r...

created [InstanceEdit:3322420] Rothfels, K, 2013-05-03
dbId 3322421
displayName SMARCA4, one of two ATPase components of SWI/SNF chromatin r...
modified [InstanceEdit:3777177] Rothfels, K, 2013-06-26
schemaClass Summation
text SMARCA4, one of two ATPase components of SWI/SNF chromatin remodelling complexes, was identified in a two-hybrid screen for beta-catenin interactors that activate TCF/LEF-dependent transcription (Barker et al, 2001; Wilson and Roberts, 2011). SMARCA4 co-immunoprecipitates with beta-catenin when both tagged proteins are expressed in HEK293 cells; likewise, tagged SMARCA4 co-precipitates endogenous beta-catenin from a colon carcinoma cell line. Expression of SMARCA4 enhances the expression of a TCF reporter in a manner that depends on a functional ATPase domain, indicating a probable role for chromatin remodelling at WNT-responsive promoters (Barker et al, 2001). By ChIP, SMARCA4 and beta-catenin are associated with WNT-responsive promoters upon WNT pathway stimulation; this study also identified TERT, a telomerase reverse transcriptase, as SMARCA4-interacting protein that is present at WNT promoters (Park et al, 2009).
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