Reactome | Retroviruses use cellular tRNAs as primers for reverse trans...

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created	[InstanceEdit:177417] D'Eustachio, P, 2006-03-30 15:26:43
dbId	177465
displayName	Retroviruses use cellular tRNAs as primers for reverse trans...
literatureReference	[LiteratureReference:177452] Identification of tRNAs incorporated into wild-type and mutant human immunodeficiency virus type 1 [LiteratureReference:182855] Primer tRNAs for reverse transcription [LiteratureReference:182839] Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles [LiteratureReference:182851] Site-specific crosslinking of 4-thiouridine-modified human tRNA(3Lys) to reverse transcriptase from human immunodeficiency virus type I
modified	[InstanceEdit:182820] Gopinathrao, G, 2006-07-19 16:21:19
schemaClass	Summation
text	Retroviruses use cellular tRNAs as primers for reverse transcription of the viral genomic RNA (Mak and Kleiman 1997). The primer tRNA is selectively packaged during assembly of retrovirus particles. In the case of HIV-1, lysine tRNAs are preferentially incorporated during retroviral packaging, and lysine tRNA 3, the specific isoacceptor form that serves as a primer for reverse transcription, anneals to the PBS (primer binding site) within the U5 region of the viral genomic RNA. This association appears to be mediated by the viral reverse transcriptase (RT) protein, possibly its "thumb" and "connection" domains (Jiang et al. 1993; Mak et al. 1994; Mishima and Steitz 1995).

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