CDT1-mediated loading of MCM2-7 to replication origin in budding yeast

Stable Identifier
R-SCE-9749345
Type
Reaction [binding]
Species
Saccharomyces cerevisiae
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
CDT1-mediated loading of MCM2-7 to replication origin in budding yeast
Based on yeast studies, CDT1 binding to the MCM2-7 complex induces a structural change in the MCM2-7 that relieves MCM2-7 autoinhibition by the C-terminus of its MCM6 subunit, enabling a direct association between MCM2-7 and CDC6 bound to the ORC(1-6) complex at the replication origin (Fernández-Cid et al. 2013). As a result, the OCCM complex is formed, which contains ORC(1-6), CDC6, CDT1 and MCM2-7 bound to the replication origin (Fernández-Cid et al. 2013, Sun et al. 2013).
Literature References
PubMed ID Title Journal Year
23603117 An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly

Fernández-Cid, A, Riera, A, Tognetti, S, Herrera, MC, Samel, S, Evrin, C, Winkler, C, Gardenal, E, Uhle, S, Speck, C

Mol Cell 2013
23851460 Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA

Sun, J, Evrin, C, Samel, SA, Fernández-Cid, A, Riera, A, Kawakami, H, Stillman, B, Speck, C, Li, H

Nat Struct Mol Biol 2013
Participants
Input
Output
Orthologous Events
CDT1-mediated loading of MCM2-7 to replication origins (Homo sapiens)
Authored
Kusic-Tisma, J  (2021-10-27)
Created
Orlic-Milacic, M  (2021-08-01)
Cite Us!