CDT1-mediated loading of MCM2-7 to replication origin in budding yeast

Stable Identifier
R-SCE-9749345
Type
Reaction [binding]
Species
Saccharomyces cerevisiae
Compartment
nucleoplasm
ReviewStatus
5/5
General
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PNG
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 | PPTX  | SBGN
CDT1-mediated loading of MCM2-7 to replication origin in budding yeast
Based on yeast studies, CDT1 binding to the MCM2-7 complex induces a structural change in the MCM2-7 that relieves MCM2-7 autoinhibition by the C-terminus of its MCM6 subunit, enabling a direct association between MCM2-7 and CDC6 bound to the ORC(1-6) complex at the replication origin (Fernández-Cid et al. 2013). As a result, the OCCM complex is formed, which contains ORC(1-6), CDC6, CDT1 and MCM2-7 bound to the replication origin (Fernández-Cid et al. 2013, Sun et al. 2013).
Literature References
PubMed ID Title Journal Year
23603117 An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly

Fernández-Cid, A, Speck, C, Samel, S, Gardenal, E, Winkler, C, Riera, A, Herrera, MC, Evrin, C, Uhle, S, Tognetti, S

Mol Cell 2013
23851460 Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA

Fernández-Cid, A, Li, H, Samel, SA, Speck, C, Riera, A, Sun, J, Kawakami, H, Evrin, C, Stillman, B

Nat Struct Mol Biol 2013
Participants
Input
Output
Orthologous Events
CDT1-mediated loading of MCM2-7 to replication origins (Homo sapiens)
Authored
Kusic-Tisma, J  (2021-10-27)
Created
Orlic-Milacic, M  (2021-08-01)
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