Precursor proteins enter TIM23 SORT complex

Stable Identifier
Reaction [binding]
Saccharomyces cerevisiae
SVG |   | PPTX  | SBGN
Precursor proteins enter TIM23 SORT complex

The TIM23 Complex resides in the inner membrane of the mitochondrion and transfers precursor proteins to the inner membrane. The TIM23 complex appears to adopt different configurations (and perhaps different subunit compositions) depending on whether the substrate is destined for the inner membrane or the matrix. Here we refer to the TIM23 SORT complex as the configuration that delivers inner membrane proteins. The TIM21 subunit is required for this activity.The N-terminal presequence of precursors first interacts with TIM50 and TIM23 (Mokranjac et al. 2003, Martinez-Caballero et al. 2007, Tamura et al. 2009). The TIM17 and TIM23 subunits form a channel and are required to initiate translocation of precursors (Milislav et al. 2001).
Substrates of the TIM23 SORT complex targeted to the inner membrane include CYB2 (Krayl et al. 2007, Hutu et al. 2008, Popov-Celeketic et al. 2008, Tamura et al. 2009, Mokranjac et al. 2009), DLD (Mokranjac et al. 2009), ATP9 (Leuenberger et al. 1999, Milisav et al. 2001, Tamura et al. 2009), COQ2 (Leuenberger et al. 1999), TIM54 (Leuenberger et al. 1999, Kurz et al. 1999), COX4 (Martinez-Caballero et al. 2007), COX5A (Mokranjac et al. 2003), and ATP2 (Dekker et al. 1997, Mokranjac et al. 2003).

Literature References
PubMed ID Title Journal Year
17148445 Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23

Martinez-Caballero, S, Grigoriev, SM, Herrmann, JM, Campo, ML, Kinnally, KW

J Biol Chem 2007
15797382 Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17

Chacinska, A, Lind, M, Frazier, AE, Dudek, J, Meisinger, C, Geissler, A, Sickmann, A, Meyer, HE, Truscott, KN, Guiard, B, Pfanner, N, Rehling, P

Cell 2005
10469659 Different import pathways through the mitochondrial intermembrane space for inner membrane proteins

Leuenberger, D, Bally, NA, Schatz, G, Koehler, CM

EMBO J 1999
9312000 The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70-Tim44

Dekker, PJ, Martin, F, Maarse, AC, Bomer, U, Müller, H, Guiard, B, Meijer, M, Rassow, J, Pfanner, N

EMBO J 1997
17074805 A cooperative action of the ATP-dependent import motor complex and the inner membrane potential drives mitochondrial preprotein import

Krayl, M, Lim, JH, Martin, F, Guiard, B, Voos, W

Mol Cell Biol 2007
18070913 Sorting switch of mitochondrial presequence translocase involves coupling of motor module to respiratory chain

Wiedemann, N, van der Laan, M, Hutu, DP, Rehling, P, Pfanner, N

J Cell Biol 2007
19144822 Role of Tim50 in the transfer of precursor proteins from the outer to the inner membrane of mitochondria

Mokranjac, D, Sichting, M, Popov-Celeketi?, D, Mapa, K, Gevorkyan-Airapetov, L, Zohary, K, Hell, K, Azem, A, Neupert, W

Mol Biol Cell 2009
19884344 Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sorting

Chacinska, A, van der Laan, M, Mehnert, CS, Guiard, B, Mick, DU, Hutu, DP, Truscott, KN, Wiedemann, N, Meisinger, C, Pfanner, N, Rehling, P

Mol Cell Biol 2010
19139266 Tim23-Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import

Tamura, Y, Harada, Y, Shiota, T, Yamano, K, Watanabe, K, Yokota, M, Yamamoto, H, Sesaki, H, Endo, T

J Cell Biol 2009
10397776 Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway

Kurz, M, Martin, H, Rassow, J, Pfanner, N, Ryan, MT

Mol Biol Cell 1999
18418384 Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria

Popov-Celeketi?, D, Mapa, K, Neupert, W, Mokranjac, D

EMBO J 2008
12574118 Tim50, a novel component of the TIM23 preprotein translocase of mitochondria

Mokranjac, D, Paschen, SA, Kozany, C, Prokisch, H, Hoppins, SC, Nargang, FE, Neupert, W, Hell, K

EMBO J 2003
1350514 Cytochromes c1 and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism

Glick, BS, Brandt, A, Cunningham, K, Müller, S, Hallberg, RL, Schatz, G

Cell 1992
8654364 Differential requirement for the mitochondrial Hsp70-Tim44 complex in unfolding and translocation of preproteins

Voos, W, von Ahsen, O, Müller, H, Guiard, B, Rassow, J, Pfanner, N

EMBO J 1996
17828250 Motor-free mitochondrial presequence translocase drives membrane integration of preproteins

van der Laan, M, Meinecke, M, Dudek, J, Hutu, DP, Lind, M, Perschil, I, Guiard, B, Wagner, R, Pfanner, N, Rehling, P

Nat Cell Biol 2007
11344168 Modular structure of the TIM23 preprotein translocase of mitochondria

Milisav, I, Moro, F, Neupert, W, Brunner, M

J Biol Chem 2001
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