Reactome | TOM40:TOM70 complex translocates proteins from the cytosol to the mitochondrial intermembrane space

TOM40:TOM70 complex translocates proteins from the cytosol to the mitochondrial intermembrane space

Stable Identifier

R-SCE-1252255

Type

Reaction [transition]

Species

Saccharomyces cerevisiae

Compartment

cytosol, mitochondrial intermembrane space, mitochondrial outer membrane

ReviewStatus

5/5

General

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TOM40:TOM70 complex translocates proteins from the cytosol to the mitochondrial intermembrane space

The TOM40:TOM70 Complex transports precursor proteins from the cytosol, across the outer membrane of the mitochondrion, and into the intermembrane space from where they may be targeted to all locations within the mitochondrion. TOM40, TOM22, TOM5, TOM6, and TOM7 form the general import pore across the membrane (Dekker et al. 1998). TOM20 and TOM22 interact with presequences on mitochondrial precursors (Haucke et al. 1995, Brix et al. 1997, Meisinger et al. 2001, Yamano et al. 2008). TOM70 interacts with hydrophobic sequences in mature internal regions of mitochondrial proteins (Schlossman et al. 1994, Brix et al. 1997, Yamamoto et al. 2009).
Substrates of the TOM40:TOM70 complex include ATP1 (Yamamoto et al. 2009), ATP2 (Yamamoto et al. 2009), ATP9 (Hines et al. 1990), TOM40 (Yamamoto et al. 2009), SSC1 (Yamamoto et al. 2009), CIT1 (Yamamoto et al. 2009), ACO1 (Yamamoto et al. 2009), IDH1 (Yamamoto et al. 2009), BCS1 (Stan et al. 2003), CYT1 (Diekert et al. 2001), TIM54 (Kurz et al. 1999), TIM22 (Kurz et al. 1999), AAC (Sollner et al. 1990, Kurz et al. 1990, Kubrich et al. 1998, Endres et al. 1999, Truscott et al. 2002, Vasiljev et al. 2004), HSP60 (Yamano et al. 2008), CYB2 (Krayl et al. 2007). Some proteins such as Aco1, Atp1, Cit1, Idh1, and Atp2 contain both presequences that interact with TOM20 and mature regions that interact with TOM70 (Yamamoto et al. 2009).

Literature References

PubMed ID	Title	Journal	Year
7890675	The yeast mitochondrial protein import receptor Mas20p binds precursor proteins through electrostatic interaction with the positively charged presequence Schatz, G, Lithgow, T, Haucke, V, Rospert, S, Hahne, K	J Biol Chem	1995
11598006	Apocytochrome c requires the TOM complex for translocation across the mitochondrial outer membrane Ahting, U, Lill, R, de Kroon, AI, Neupert, W, Diekert, K, de Kruijff, B, Niggemeyer, B	EMBO J	2001
21896724	In vivo protein-interaction mapping of a mitochondrial translocator protein Tom22 at work Yamano, K, Tanaka-Yamano, S, Endo, T, Shiota, T, Mabuchi, H	Proc Natl Acad Sci U S A	2011
8163488	Specific recognition of mitochondrial preproteins by the cytosolic domain of the import receptor MOM72 Pfanner, N, Neupert, W, Schlossmann, J, Dietmeier, K	J Biol Chem	1994
12391147	Mitochondrial import of the ADP/ATP carrier: the essential TIM complex of the intermembrane space is required for precursor release from the TOM complex Pfanner, N, Müller, H, Rehling, P, Wiedemann, N, Guiard, B, Meisinger, C, Truscott, KN	Mol Cell Biol	2002
17074805	A cooperative action of the ATP-dependent import motor complex and the inner membrane potential drives mitochondrial preprotein import Lim, JH, Guiard, B, Krayl, M, Voos, W, Martin, F	Mol Cell Biol	2007
10369662	Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22.54 complex Neupert, W, Brunner, M, Endres, M	EMBO J	1999
19767391	Roles of Tom70 in import of presequence-containing mitochondrial proteins Yamano, K, Esaki, M, Fukui, K, Endo, T, Terao, K, Uchida, M, Yamamoto, H, Nishikawa, S, Takahashi, H, Shiota, T, Yoshihisa, T, Kitamura, S	J Biol Chem	2009
2163763	A mitochondrial import receptor for the ADP/ATP carrier Pfanner, N, Griffiths, G, Pfaller, R, Neupert, W, Söllner, T	Cell	1990
9774667	Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex Pfanner, N, Müller, H, Brix, J, Honlinger, A, Ryan, MT, Dekker, PJ	Mol Cell Biol	1998
10397776	Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway Pfanner, N, Kurz, M, Ryan, MT, Martin, H, Rassow, J	Mol Biol Cell	1999
9632701	The import route of ADP/ATP carrier into mitochondria separates from the general import pathway of cleavable preproteins at the trans side of the outer membrane Pfanner, N, Kübrich, M, Honlinger, A, Rassow, J, Voos, W	J Biol Chem	1998
14668492	Reconstituted TOM core complex and Tim9/Tim10 complex of mitochondria are sufficient for translocation of the ADP/ATP carrier across membranes Panneels, V, Ahting, U, Go, NE, Neupert, W, Habib, SJ, Rapaport, D, Nussberger, S, Kozany, C, Prokisch, H, Nargang, FE, Vasiljev, A, Sinning, I	Mol Biol Cell	2004
11259583	Protein import channel of the outer mitochondrial membrane: a highly stable Tom40-Tom22 core structure differentially interacts with preproteins, small tom proteins, and import receptors Pfanner, N, Wagner, R, Lim, JH, Sickmann, A, Müller, H, Model, K, Ryan, MT, Meyer, HE, Hill, K, Meisinger, C	Mol Cell Biol	2001
9252394	Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70 Pfanner, N, Brix, J, Dietmeier, K	J Biol Chem	1997
18063580	Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import Esaki, M, Yamano, K, Endo, T, Hobbs, AE, Yatsukawa, Y, Jensen, RE	J Biol Chem	2008
12640110	Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex Pfanner, N, Schneider-Mergener, J, Neupert, W, Brix, J, Rapaport, D, Stan, T	Mol Cell Biol	2003
2170106	Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70 Schatz, G, Horstmann, H, Griffiths, G, Brutsch, H, Hines, V, Brandt, A	EMBO J	1990