Tet3 oxidizes 5-methylcytosine to 5-hydroxymethylcytosine in chromatin containing histone H3.3 dimethylated on lysine-9

Stable Identifier
R-MMU-9817463
Type
Reaction [transition]
Species
Mus musculus
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Tet3 oxidizes 5-methylcytosine to 5-hydroxymethylcytosine in chromatin containing histone H3.3 dimethylated on lysine-9
Tet3 supplied by maternal cytoplasm is the major enzyme catalyzing oxidation of 5-methylcytidine to 5-hydroxymethylcytidine in the male pronucleus (Gu et al. 2011, Wossidlo et al. 2011, Iqbal et al. 2011, Santos et al. 2013). Tet3-catalyzed demethylation is part of the second phase of active demethylation in the male pronucleus (Santos et al. 2013). The first phase appears to be catalyzed by cytidine deamination followed by base excision repair (Santos et al. 2013). Accumulation of 5-hydroxymethylcytidine requires the cytosine methylases Dnmt3a and Dnmt1 (Amouroux et al. 2016). In mouse embryonic stem cells 5-hydroxymethylcytidine is mostly associated with euchromatin and depends on pre-existing 5-methylcytidine (Ficz et al. 2011). Tet3 is associated with Stgp4 and Mettl23 bound to chromatin (Hatanaka et al. 2017). Tet1 and Tet2 may also play a role in production of hydroxycytidine in the paternal genome (Ficz et al. 2011).
Literature References
PubMed ID Title Journal Year
26751286 De novo DNA methylation drives 5hmC accumulation in mouse zygotes

Amouroux, R, Nashun, B, Shirane, K, Nakagawa, S, Hill, PW, D'Souza, Z, Nakayama, M, Matsuda, M, Turp, A, Ndjetehe, E, Encheva, V, Kudo, NR, Koseki, H, Sasaki, H, Hajkova, P

Nat Cell Biol 2016
28930672 Histone H3 Methylated at Arginine 17 Is Essential for Reprogramming the Paternal Genome in Zygotes

Hatanaka, Y, Tsusaka, T, Shimizu, N, Morita, K, Suzuki, T, Machida, S, Satoh, M, Honda, A, Hirose, M, Kamimura, S, Ogonuki, N, Nakamura, T, Inoue, K, Hosoi, Y, Dohmae, N, Nakano, T, Kurumizaka, H, Matsumoto, K, Shinkai, Y, Ogura, A

Cell Rep 2017
21892189 The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes

Gu, TP, Guo, F, Yang, H, Wu, HP, Xu, GF, Liu, W, Xie, ZG, Shi, L, He, X, Jin, SG, Iqbal, K, Shi, YG, Deng, Z, Szabó, PE, Pfeifer, GP, Li, J, Xu, GL

Nature 2011
21321204 Reprogramming of the paternal genome upon fertilization involves genome-wide oxidation of 5-methylcytosine

Iqbal, K, Jin, SG, Pfeifer, GP, Szabó, PE

Proc. Natl. Acad. Sci. U.S.A. 2011
21407207 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming

Wossidlo, M, Nakamura, T, Lepikhov, K, Marques, CJ, Zakhartchenko, V, Boiani, M, Arand, J, Nakano, T, Reik, W, Walter, J

Nat Commun 2011
24279473 Active demethylation in mouse zygotes involves cytosine deamination and base excision repair

Santos, F, Peat, J, Burgess, H, Rada, C, Reik, W, Dean, W

Epigenetics Chromatin 2013
Participants
Input
Output
Catalyst Activity

DNA 5-methylcytosine dioxygenase activity of Tet3:Stgp4:Mettl23:chromatin with H3.3 dimethylarginine-17 and 5-mC [nucleoplasm]

Physical Entity
Tet3:Stgp4:Mettl23:chromatin with H3.3 dimethylarginine-17 and 5-mC [nucleoplasm] (Mus musculus)
Activity
DNA 5-methylcytosine dioxygenase activity (GO:0070579)
Orthologous Events
TET3 oxidizes 5-methylcytosine to 5-hydroxymethylcytosine in chromatin containing histone H3.3 (Homo sapiens)
Authored
May, B  (2022-09-18)
Reviewed
Xie, W  (2023-08-08)
Created
May, B  (2022-09-21)
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