Formation of Meiotic Single-stranded Invasion Complex

Stable Identifier
R-MMU-912421
Type
Reaction [omitted]
Species
Mus musculus
Compartment
nucleoplasm
Synonyms
Formation of Early Recombination Nodule
ReviewStatus
5/5
General
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PNG
Low
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Formation of Meiotic Single-stranded Invasion Complex
Two RecA homologs, Rad51 and the meiosis-specific Dmc1, coat single-stranded 3' ends of DNA produced by resection of double-strand breaks. Rad51 and Dmc1 interact and colocalize to the same early recombination nodules (Ashley et al. 1995, Ikeya et al. 1996, Plug et al. 1996, Barlow et al. 1997, Barlow et al. 1998, Tarsounas et al. 1999, Moens et al. 2007). Knockouts of Dmc1 abolish recombination and synapsis therefore Rad51 is not sufficient for recombination (Pittman et al. 1998, Yoshida et al. 1998).
Immunocytology shows the Rpa heterotrimer arrives at recombination nodules with or after Rad51 and Dmc1 (Plug et al. 1998, Plug et al. 1997, Moens et al. 2007). Rpa also co-localizes with Atm (Plug et al. 1997). The checkpoint kinase Cdk4 colocalizes with Rpa (Ashley et al. 2001).
Brca1 and Brca2 are found extensively distributed on synaptonemal complexes. Results from knockout mice indicate that Brca2, Rad51C, and Tex15 participate in loading Rad51 and Dmc1 onto single-stranded DNA (Kuznetsov et al. 2007, Yang et al. 2008). Brca1 participates in loading Rad51 but not Dmc1.
Literature References
PubMed ID Title Journal Year
9735362 Atm deficiency results in severe meiotic disruption as early as leptonema of prophase I

Barlow, C, Liyanage, M, Moens, PB, Tarsounas, M, Nagashima, K, Brown, K, Rottinghaus, S, Jackson, SP, Tagle, D, Ried, T, Wynshaw-Boris, A

Development 1998
9443891 Changes in protein composition of meiotic nodules during mammalian meiosis

Plug, AW, Peters, AH, Keegan, KS, Hoekstra, MF, de Boer, P, Ashley, T

J Cell Sci 1998
9660953 Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog

Pittman, DL, Cobb, J, Schimenti, KJ, Wilson, LA, Cooper, DM, Brignull, E, Handel, MA, Schimenti, JC

Mol Cell 1998
10525529 RAD51 and DMC1 form mixed complexes associated with mouse meiotic chromosome cores and synaptonemal complexes

Tarsounas, M, Morita, T, Pearlman, RE, Moens, PB

J Cell Biol 1999
17344431 Initiation and resolution of interhomolog connections: crossover and non-crossover sites along mouse synaptonemal complexes

Moens, PB, Marcon, E, Shore, JS, Kochakpour, N, Spyropoulos, B

J Cell Sci 2007
9660954 The mouse RecA-like gene Dmc1 is required for homologous chromosome synapsis during meiosis

Yoshida, K, Kondoh, G, Matsuda, Y, Habu, T, Nishimune, Y, Morita, T

Mol Cell 1998
9398850 ATM and RPA in meiotic chromosome synapsis and recombination

Plug, AW, Peters, AH, Xu, Y, Keegan, KS, Hoekstra, MF, Baltimore, D, de Boer, P, Ashley, T

Nat Genet 1997
17312021 RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females

Kuznetsov, S, Pellegrini, M, Shuda, K, Fernandez-Capetillo, O, Liu, Y, Martin, BK, Burkett, S, Southon, E, Pati, D, Tessarollo, L, West, SC, Donovan, PJ, Nussenzweig, A, Sharan, SK

J Cell Biol 2007
8650194 Presynaptic association of Rad51 protein with selected sites in meiotic chromatin

Plug, AW, Xu, J, Reddy, G, Golub, EI, Ashley, T

Proc Natl Acad Sci U S A 1996
7587590 Dynamic changes in Rad51 distribution on chromatin during meiosis in male and female vertebrates

Ashley, T, Plug, AW, Xu, J, Solari, AJ, Reddy, G, Golub, EI, Ward, DC

Chromosoma 1995
18283110 Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis

Yang, F, Eckardt, S, Leu, NA, McLaughlin, KJ, Wang, PJ

J Cell Biol 2008
11171374 Localization of two mammalian cyclin dependent kinases during mammalian meiosis

Ashley, T, Walpita, D, de Rooij, DG

J Cell Sci 2001
9135082 Localization of mouse Rad51 and Lim15 proteins on meiotic chromosomes at late stages of prophase 1

Ikeya, T, Shinohara, A, Sato, S, Tabata, S, Ogawa, T

Genes Cells 1996
9311981 Distribution of the Rad51 recombinase in human and mouse spermatocytes

Barlow, AL, Benson, FE, West, SC, Hultén, MA

EMBO J 1997
Participants
Input
Output
Orthologous Events
Formation of meiotic single-stranded DNA invasion complex (Homo sapiens)
Authored
May, B  (2010-07-03)
Reviewed
Bolcun-Filas, E  (2011-02-25)
Cohen, PE  (2011-02-05)
Holloway, JK  (2011-02-05)
Lyndaker, A  (2011-02-25)
Schimenti, JC  (2011-02-05)
Strong, E  (2011-02-25)
Created
May, B  (2010-07-09)
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