Unknown nuclease cleaves pre-piRNA in Asz1:Ddx4:Mov10l1:6xMeR-Piwil2:pre-piRNA:Tdrd1:Tdrd12

Stable Identifier
Reaction [omitted]
Mus musculus
SVG |   | PPTX  | SBGN
Unknown nuclease cleaves pre-piRNA in Asz1:Ddx4:Mov10l1:6xMeR-Piwil2:pre-piRNA:Tdrd1:Tdrd12

After binding Piwil2 (Mili in mouse, HILI in human) the 3' end of the pre-piRNA is trimmed by an unknown nuclease (Aravin et al. 2006, Grivna et al. 2006, Aravin et al. 2008, Vourekas et al. 2012). The final size of the piRNA appears to be determined by the particular PIWI protein with which it is associated (reviewed in Siomi et al. 2011). The existence of a magnesium-dependent trimming activity is inferred from in vitro reactions using proteins of silkworm (Bombyx mori) ovary cells (Kawaoka et al. 2011). Mov10l1, which has a helicase domain, associates with Piwil2 and is required for loading Piwil2 with piRNA (Zheng et al. 2010, Frost et al. 2010). Piwil2, Tdrd1, Mvh, and Asz (Gasz) are located in the intermitochondrial cement, the chromatoid body, and the pi-body, a type of nuage (electron-dense perinuclear material) (Aravin et al. 2009, reviewed in Pillai and Chuma 2012).

Literature References
PubMed ID Title Journal Year
21427766 PIWI-interacting small RNAs: the vanguard of genome defence

Siomi, MC, Sato, K, Pezic, D, Aravin, AA

Nat. Rev. Mol. Cell Biol. 2011
22221002 piRNAs and their involvement in male germline development in mice

Pillai, RS, Chuma, S

Dev. Growth Differ. 2012
20547853 MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs

Frost, RJ, Hamra, FK, Richardson, JA, Qi, X, Bassel-Duby, R, Olson, EN

Proc. Natl. Acad. Sci. U.S.A. 2010
16751777 A novel class of small RNAs bind to MILI protein in mouse testes

Aravin, A, Gaidatzis, D, Pfeffer, S, Lagos-Quintana, M, Landgraf, P, Iovino, N, Morris, P, Brownstein, MJ, Kuramochi-Miyagawa, S, Nakano, T, Chien, M, Russo, JJ, Ju, J, Sheridan, R, Sander, C, Zavolan, M, Tuschl, T

Nature 2006
22842725 Mili and Miwi target RNA repertoire reveals piRNA biogenesis and function of Miwi in spermiogenesis

Vourekas, A, Zheng, Q, Alexiou, P, Maragkakis, M, Kirino, Y, Gregory, BD, Mourelatos, Z

Nat. Struct. Mol. Biol. 2012
18922463 A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice

Aravin, AA, Sachidanandam, R, Bourc'his, D, Schaefer, C, Pezic, D, Toth, KF, Bestor, T, Hannon, GJ

Mol. Cell 2008
21925389 3' end formation of PIWI-interacting RNAs in vitro

Kawaoka, S, Izumi, N, Katsuma, S, Tomari, Y

Mol. Cell 2011
20011505 Cytoplasmic compartmentalization of the fetal piRNA pathway in mice

Aravin, AA, van der Heijden, GW, CastaƱeda, J, Vagin, VV, Hannon, GJ, Bortvin, A

PLoS Genet. 2009
20534472 Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway

Zheng, K, Xiol, J, Reuter, M, Eckardt, S, Leu, NA, McLaughlin, KJ, Stark, A, Sachidanandam, R, Pillai, RS, Wang, PJ

Proc. Natl. Acad. Sci. U.S.A. 2010
16766680 A novel class of small RNAs in mouse spermatogenic cells

Grivna, ST, Beyret, E, Wang, Z, Lin, H

Genes Dev. 2006
Orthologous Events
Cite Us!