SGO1 (also known as SGOL1 or Shugoshin 1), a protein essential for cohesion of sister chromatids and proper seggregation of duplicated chromosomes during mitosis, was found to bind to CCNA2 specifically in G2 as compared with S phase (Pagliuca et al. 2011) and was reproducibly identified as a substrate of CCNA:CDK1 (Dumitru et al. 2017).
SGO1 was shown to be phosphorylated by CDK1 in vivo at an evolutionarily conserved consensus CDK1/2 site T346, and this site was shown to be phosphorylated in vitro by the CCNB1:CDK1 complex, while CCNA:CDK1 complexes were not tested as catalysts (Liu et al. 2013). CDK1-mediated phosphorylation of SGO1 facilitates SGO1-mediated recruitment of PP2A to the cohesin complex, thus preventing premature separation of duplicated chromosomes (Liu et al. 2013).
Dumitru, AMG, Rusin, SF, Clark, AEM, Kettenbach, AN, Compton, DA
Liu, H, Rankin, S, Yu, H
Pagliuca, FW, Collins, MO, Lichawska, A, Zegerman, P, Choudhary, JS, Pines, J
cyclin-dependent protein serine/threonine kinase activity of p-S-CCNA:p-T161-CDK1 [nucleoplasm]
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