NS5 binds NS3

Stable Identifier
R-HSA-9920215
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Dengue virus type 2
Compartment
cytosol
ReviewStatus
5/5
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NS5 binds NS3
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DENV NS3 and NS5 interact (Takahashi et al., 2012). The polymerase activity of NS5 probably requires the helicase and the NTPase activity of NS3 to replicate the genome. Also, 5′-end capping of nascent RNA involves coordinated activities of NS3 (RNA 5′-triphosphatase) and the NS5 N-terminal SAM-dependent methyltransferase (N7- and 2′-O methylation) (Johansson et al., 2001 using DENV-2 strain TSV01).
Literature References
PubMed ID Title Journal Year
23072882 Establishment of a robust dengue virus NS3-NS5 binding assay for identification of protein-protein interaction inhibitors

Takahashi, H, Takahashi, C, Moreland, NJ, Chang, YT, Sawasaki, T, Ryo, A, Vasudevan, SG, Suzuki, Y, Yamamoto, N

Antiviral Res 2012
11257177 A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin-beta and the viral helicase, NS3

Johansson, M, Brooks, AJ, Jans, DA, Vasudevan, SG

J Gen Virol 2001
37478848 Structures of dengue virus RNA replicase complexes

Osawa, T, Aoki, M, Ehara, H, Sekine, SI

Mol Cell 2023
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
dengue disease DOID:12205 breakbone fever, Dengue Fever, classic dengue
Cross References
Mondo
Authored
Stephan, R  (2024-09-06)
Reviewed
Mansur, DS  (2025-09-18)
Roque Ascenção, F  (2025-10-29)
Roque Ascenção, F  (2026-03-03)
Created
Orlic-Milacic, M  (2024-08-29)
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