Dengue polyprotein cis-auto-cleaves

Stable Identifier
R-HSA-9918772
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Dengue virus type 2
Compartment
endoplasmic reticulum membrane
ReviewStatus
5/5
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Dengue polyprotein cis-auto-cleaves
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The NS3 peptidase domain of the folded Dengue virus polyprotein, together with the upstream NS2B cofactor, can process the polyprotein "in cis" through autolytic cleavage. This has been demonstrated by cis-processing of DENV-2 polyprotein constructs at sites including NS2A/NS2B and NS2B/NS3 in vitro (Preugschat et al., 1990), and through cis autolytic cleavage at the NS2B/NS3 junction by recombinant Dengue virus (DENV-2, DENV-4) NS2B-NS3 proteins (Bera et al., 2007). Such NS3-mediated processing of DENV-2 polyprotein constructs results in the production of the C-prM-E-NS1-NS2A fragment, NS2B, and the NS345 fragment (Preugschat & Strauss, 1991). The ability of recombinant DENV-2 NS2B-NS3 to perform cis autolytic cleavage at the NS2B/NS3 junction contributes to forming NS2B and the NS3-containing fragment (Bera et al., 2007), and this enzyme can also cleave peptide substrates representing the NS2A/NS2B and NS2B/NS3 junctions (Shiryaev et al., 2007).
Literature References
PubMed ID Title Journal Year
17337448 Functional characterization of cis and trans activity of the Flavivirus NS2B-NS3 protease

Bera, AK, Kuhn, RJ, Smith, JL

J Biol Chem 2007
17067286 Cleavage preference distinguishes the two-component NS2B-NS3 serine proteinases of Dengue and West Nile viruses

Shiryaev, SA, Kozlov, IA, Ratnikov, BI, Smith, JW, Lebl, M, Strongin, AY

Biochem J 2007
1683727 Processing of nonstructural proteins NS4A and NS4B of dengue 2 virus in vitro and in vivo

Preugschat, F, Strauss, JH

Virology 1991
7941319 Processing of flavivirus structural glycoproteins: stable membrane insertion of premembrane requires the envelope signal peptide

Markoff, L, Chang, A, Falgout, B

Virology 1994
2143543 In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3

Preugschat, F, Yao, CW, Strauss, JH

J Virol 1990
Participants
Input
Output
Participates
as an event of
Catalyst Activity

serine-type endopeptidase activity of folded HyPro-Polyprotein [endoplasmic reticulum membrane]

Physical Entity
folded HyPro-Polyprotein [endoplasmic reticulum membrane] (Dengue virus type 2)
Activity
serine-type endopeptidase activity (GO:0004252)
Disease
Name Identifier Synonyms
dengue disease DOID:12205 breakbone fever, Dengue Fever, classic dengue
Cross References
Mondo
Authored
Stephan, R  (2024-08-18)
Reviewed
Mansur, DS  (2025-09-18)
Roque Ascenção, F  (2025-10-29)
Created
Stephan, R  (2024-08-18)
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