SARS-CoV-2 N binds to G3BP1,G3BP2

Stable Identifier
R-HSA-9729481
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
Compartment
ReviewStatus
5/5
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Stress granules (SGs) are dynamic large cytoplasmic mRNA-protein aggregates, formed in response to various stresses, including heat shock, oxidative stress, or viral infection. They can counter viral infection by insulating invaders and limiting viral mRNA translation. SARS-CoV-2 nucleocapsid (N) protein interacts with the SG core component G3BP1/2 (Nabeel-Shah et al. 2020; Gordon et al. 2020; Luo et al. 2021; Wang et al. 2021) and almost completely disrupts SG formation. GST pull-down assays with wild-type and truncated proteins indicated that a fragment from the N-terminal intrinsically disordered region (N-IDR, aa1–48) of N protein and the nuclear transport factor 2 (NTF2) domain of G3BP1are required for the interaction (Huang W et al. 2021). N protein impaired the interaction between G3BP1 and other SG-related proteins and led to SG reconstitution through phase separating with G3BP to occupy protein–protein interaction positions of G3BP (Huang W et al. 2021). It is proposed that N protein-mediated SG disassembly is crucial for SARS-CoV-2 production (Luo et al. 2021; Wang et al. 2021).
Literature References
PubMed ID Title Journal Year
  SARS-CoV-2 Nucleocapsid protein attenuates stress granule formation and alters gene expression via direct interaction with host mRNAs

Zhang, Z, Wei, H, Farhangmehr, S, Ashraf, K, Lee, H, Zhong, G, Ahmed, N, Tang, H, Burke, GL, Greenblatt, F, Yang, J, Blencowe, J, Pu, S, Nabeel-Shah, S

   
33479219 SARS-CoV-2 nucleocapsid protein undergoes liquid-liquid phase separation into stress granules through its N-terminal intrinsically disordered region

Wang, J, Yin, H, Shi, C, Xu, Q

Cell Discov 2021
33495715 SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production

Jin, Z, Zhao, T, Ju, X, Huang, X, Ma, P, Ding, Q, Li, P, Xu, X, Chi, T, Li, Z, Zhang, Y, Luo, L, Jin, B, Huang, C, Zou, Y, Liu, J, He, S, Huang, J, Liang, A, Zhou, Y

Sci Bull (Beijing) 2021
34400613 Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein

Yu, Y, Ju, X, Jia, D, Ding, Q, Sun, Q, Li, X, Tian, M, Huang, W

Cell Discov 2021
32353859 A SARS-CoV-2 protein interaction map reveals targets for drug repurposing

Foussard, H, Beltrao, P, Koh, C, Vignuzzi, M, Peng, S, Subramanian, A, Richards, AL, Kuzuoglu-Ozturk, D, Bouhaddou, M, Melnyk, JE, Ruggero, D, Wang, HY, Venkataramanan, S, Tummino, TA, Meyer, B, O'Neal, MC, Wenzell, NA, Guo, JZ, Barrio-Hernandez, I, Huang, XP, Verba, KA, Chorba, JS, Emerman, M, Ideker, T, Lyu, J, Hernandez-Armenta, C, Fabius, JM, Obernier, K, Safari, M, Craik, CS, Dai, SA, Kim, M, Perica, T, Haas, P, Moreno, E, Zhang, Z, Cakir, M, Soucheray, M, Liu, Y, Cai, Y, Hüttenhain, R, Rosenthal, SB, Hiatt, J, Chang, JCJ, Ashworth, A, Calviello, L, Ott, M, Kortemme, T, Klippsten, S, Kaake, RM, Jang, GM, Broadhurst, DJ, Memon, D, Gordon, DE, Roth, TL, d'Enfert, C, Shi, Y, Floor, SN, Frankel, AD, Fujimori, DG, Trenker, R, Vallet, T, McGregor, MJ, Mukherjee, S, García-Sastre, A, Fletcher, SJ, Fraser, JS, Xu, J, Saltzberg, DJ, Sharp, PP, White, KM, Kirby, IT, Braberg, H, Schwartz, O, Lin, Y, Naing, ZZC, Shoichet, BK, Noack, J, Mac Kain, A, Polacco, BJ, Lou, K, Rosenberg, OS, Mathy, CJP, Bennett, MJ, Shen, W, Ganesan, SJ, Bohn, M, Savar, NS, Cavero, DA, Haas, K, Stroud, RM, Eckhardt, M, Li, Q, Young, JM, O'Meara, MJ, Miorin, L, Liboy-Lugo, J, Swaney, DL, Gross, JD, Pilla, KB, von Zastrow, M, Jacobson, M, Tutuncuoglu, B, Roesch, F, Shokat, KM, Rezelj, VV, Zhou, Y, Krogan, NJ, Roth, BL, Agard, DA, Ugur, FS, Sali, A, Malik, HS, Liu, X, Jura, N, Taunton, J, Verdin, E, Batra, J, Hubert, M, Rathore, U, Rakesh, R, Tran, QD, Shengjuler, D, Modak, M, Wankowicz, SA

Nature 2020
Participants
Participates
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Authored
Reviewed
Created
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