SARS-CoV-2 nsp13 binds TBK1

Stable Identifier
R-HSA-9705082
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
Compartment
ReviewStatus
5/5
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Viral infection and stimulation of some pattern-recognition receptors such as the toll-like receptor 3 (TLR3) in the endosomes or the RIG-I-like receptors in the cytosol leads to the activation of the IKK-related TANK binding kinase 1 (TBK1). TBK1 in turn phosphorylates the interferon regulatory factor 3 (IRF3/7). The phosphorylated IRF3 forms a dimer and translocates to the nucleus, activating the transcription of type I IFN (IFN-I) genes (Tu D et al. 2013). Non-structural protein 13 (nsp13) originated from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was found to interact with TBK1 in human embryonic kidney 293 (HEK293) cells, that were co-transfected with plasmids expressing TBK1 and tagged nsp13 (Xia H et al. 2020; Gordon DE et al. 2020). Viral nsp13 antagonized IFN-I production in HEK293 cells by binding to TBK1 and suppressing phosphorylation of TBK1 (Xia H et al. 2020; Vazquez et al, 2021).
Literature References
PubMed ID Title Journal Year
32979938 Evasion of Type I Interferon by SARS-CoV-2

Chen, JY, Rajsbaum, R, Zhang, X, Xia, H, Cao, Z, Wang, H, Menachery, VD, Shi, PY, Xie, X

Cell Rep 2020
34166398 SARS-CoV-2 viral proteins NSP1 and NSP13 inhibit interferon activation through distinct mechanisms

Negatu, SG, Miller, J, Ramage, HR, Swanson, SE, Cherry, S, Dittmar, M, Jurado, KA, Vazquez, C

PLoS One 2021
32353859 A SARS-CoV-2 protein interaction map reveals targets for drug repurposing

Foussard, H, Beltrao, P, Koh, C, Vignuzzi, M, Peng, S, Subramanian, A, Richards, AL, Kuzuoglu-Ozturk, D, Bouhaddou, M, Melnyk, JE, Ruggero, D, Wang, HY, Venkataramanan, S, Tummino, TA, Meyer, B, O'Neal, MC, Wenzell, NA, Guo, JZ, Barrio-Hernandez, I, Huang, XP, Verba, KA, Chorba, JS, Emerman, M, Ideker, T, Lyu, J, Hernandez-Armenta, C, Fabius, JM, Obernier, K, Safari, M, Craik, CS, Dai, SA, Kim, M, Perica, T, Haas, P, Moreno, E, Zhang, Z, Cakir, M, Soucheray, M, Liu, Y, Cai, Y, Hüttenhain, R, Rosenthal, SB, Hiatt, J, Chang, JCJ, Ashworth, A, Calviello, L, Ott, M, Kortemme, T, Klippsten, S, Kaake, RM, Jang, GM, Broadhurst, DJ, Memon, D, Gordon, DE, Roth, TL, d'Enfert, C, Shi, Y, Floor, SN, Frankel, AD, Fujimori, DG, Trenker, R, Vallet, T, McGregor, MJ, Mukherjee, S, García-Sastre, A, Fletcher, SJ, Fraser, JS, Xu, J, Saltzberg, DJ, Sharp, PP, White, KM, Kirby, IT, Braberg, H, Schwartz, O, Lin, Y, Naing, ZZC, Shoichet, BK, Noack, J, Mac Kain, A, Polacco, BJ, Lou, K, Rosenberg, OS, Mathy, CJP, Bennett, MJ, Shen, W, Ganesan, SJ, Bohn, M, Savar, NS, Cavero, DA, Haas, K, Stroud, RM, Eckhardt, M, Li, Q, Young, JM, O'Meara, MJ, Miorin, L, Liboy-Lugo, J, Swaney, DL, Gross, JD, Pilla, KB, von Zastrow, M, Jacobson, M, Tutuncuoglu, B, Roesch, F, Shokat, KM, Rezelj, VV, Zhou, Y, Krogan, NJ, Roth, BL, Agard, DA, Ugur, FS, Sali, A, Malik, HS, Liu, X, Jura, N, Taunton, J, Verdin, E, Batra, J, Hubert, M, Rathore, U, Rakesh, R, Tran, QD, Shengjuler, D, Modak, M, Wankowicz, SA

Nature 2020
Participants
Participates
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Authored
Reviewed
Created
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