Reactome | NPAS4 binds ARNT

NPAS4 binds ARNT

Stable Identifier

R-HSA-9634850

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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NPAS4 is a basic-helix-loop-helix (bHLH) transcription factor that needs to dimerize with another bHLH protein in order to be able to bind to target DNA. ARNT (ARNT1) is a bHLH transcription factor that can form a transcriptionally active heterodimer with NPAS4 (Ooe et al. 2004). In vivo, endogenous Arnt and Npas4 can be co-immunoprecipitated from the mouse brain (Ooe et al. 2009) and from primary cultures of mouse brain slices (Brigidi et al. 2019). Based on a study using primary cultures of mouse brain slices, Npas4 induced by excitatory post-synaptic potentials (EPSPs) exclusively associates with Arnt both at 5 min, when Npas4 is in the dendrites and 60 min post-stimulation, after Npas4 has translocated to the nucleus. Npas4 and Arnt mRNAs co-localize in the dendrites and may be translated concurrently (Brigidi et al. 2019).

Literature References

PubMed ID	Title	Journal	Year
14701734	Identification of a novel basic helix-loop-helix-PAS factor, NXF, reveals a Sim2 competitive, positive regulatory role in dendritic-cytoskeleton modulator drebrin gene expression Nakatuka, I, Ooe, N, Mikami, N, Kaneko, H, Saito, K	Mol. Cell. Biol.	2004
31585079	Genomic Decoding of Neuronal Depolarization by Stimulus-Specific NPAS4 Heterodimers Lin, PA, Brigidi, GS, Hayes, MGB, Bartlett, A, Bloodgood, BL, Heinz, S, Texari, L, Ecker, JR, Delos Santos, NP, Benner, C, Hartzell, AL	Cell	2019