FTSJ1 2'-O-methylates guanosine-34 in tRNA(Phe)

Stable Identifier
R-HSA-9024161
Type
Reaction [transition]
Species
Homo sapiens
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FTSJ1 methylates the 2'‑hydroxyl group of cytidine‑32 and guanosine‑34 in the anticodon loop of tRNA(Phe), as well as C32 and N34 of other substrate tRNAs (Guy and Phizicky 2015a, Guy et al. 2015b). Based on the functional and sequence homology between human FTSJ1 and yeast Trm7 the reaction is inferred to occur in the cytosol. THADA is required together with FTSJ1 for the methylation reaction at C32, based on its complementation of trm732 mutants in yeast, but the function of THADA has not been directly demonstrated in human cells (Guy and Phizicky 2015a).

Literature References
PubMed ID Title Journal Year
26310293 Defects in tRNA Anticodon Loop 2'-O-Methylation Are Implicated in Nonsyndromic X-Linked Intellectual Disability due to Mutations in FTSJ1

Guy, MP, Shaw, M, Weiner, CL, Hobson, L, Stark, Z, Rose, K, Kalscheuer, VM, Gecz, J, Phizicky, EM

Hum. Mutat. 2015
25404562 Conservation of an intricate circuit for crucial modifications of the tRNAPhe anticodon loop in eukaryotes

Guy, MP, Phizicky, EM

RNA 2015
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Catalyst Activity
Title
tRNA (guanosine-2'-O-)-methyltransferase activity of FTSJ1 [cytosol]
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