TPST1,2 transfer SO4(2-) from PAPS to PODXL2

Stable Identifier
Reaction [transition]
Homo sapiens
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Podocalyxin-like protein 2 (PODXL2 aka endoglycan) acts as a ligand for vascular selectins which mediate the rapid rolling of leukocytes over vascular surfaces. PODXL2 interacts with selectins through sulfation on two tyrosine residues (97 and 118) (Fieger et al. 2003, Kerr et al. 2008) and O-linked carbohydrate structures within its acidic amino-terminal region (latter not shown here). Protein-tyrosine sulfotransferases 1 and 2 (TPST1 and TPST2) are Golgi membrane-resident proteins which catalyse the transfer of sulfate from 3'-phospho-5'-adenylyl sulfate (PAPS) to tyrosine residues within acidic motifs of polypeptides such as PODXL2 (Ouyang et al. 1998, Danan et al. 2008, Teramoto et al. 2013).

Literature References
PubMed ID Title Journal Year
18606703 Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins

Fieger, CB, Snapp, KR, Kerr, SC, Rosen, SD

J. Immunol. 2008
23481380 Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction

Adachi, R, Nakanishi, Y, Kurogi, K, Kawaguchi, Y, Kakuta, Y, Suiko, M, Sakakibara, Y, Kimura, M, Liu, MC, Fujikawa, Y, Soejima, M, Teramoto, T, Mishiro-Sato, E

Nat Commun 2013
18672380 Mass spectrometric kinetic analysis of human tyrosylprotein sulfotransferase-1 and -2

Moore, KL, Danan, LM, Hoffhines, AJ, Leary, JA, Yu, Z

J. Am. Soc. Mass Spectrom. 2008
12889478 Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x

Fieger, CB, Sassetti, CM, Rosen, SD

J. Biol. Chem. 2003
9501187 Tyrosylprotein sulfotransferase: purification and molecular cloning of an enzyme that catalyzes tyrosine O-sulfation, a common posttranslational modification of eukaryotic proteins

Lane, WS, Ouyang, Yb, Moore, KL

Proc. Natl. Acad. Sci. U.S.A. 1998
Catalyst Activity

protein-tyrosine sulfotransferase activity of TPST1,2 [Golgi membrane]

Orthologous Events
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