RNF144A polyubiquitinates PRKDC

Stable Identifier
Reaction [omitted]
Homo sapiens
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The ubiquitin E3 ligase RNF144A transfers ubiquitin from the E2-ubiquitin conjugate UBE2L3:Ubiquitin to an unknown residue of PRKDC (DNA-PKcs, the catalytic subunit of DNA-dependent protein kinase) (Ho et al. 2014). RNF144A polyubiquitinates PRKDC with lysine-48 linked ubiquitin, leading to proteasomal degradation of PRKDC. Expression of RNF144A is activated by TP53 (p53) and the degradation of PRKDC caused by RNF144A may be pro-apoptotic (Ho et al. 2014). RNF144A contains a transmembrane domain that localizes RNF144A to endosomal membranes (Ho et al. 2015).

Literature References
PubMed ID Title Journal Year
24979766 RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage

Lin, WC, Mahanic, CS, Ho, SR, Lee, YJ

Proc. Natl. Acad. Sci. U.S.A. 2014
26216882 Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain

Lin, WC, Ho, SR, Lee, YJ

J. Biol. Chem. 2015
Catalyst Activity

ubiquitin protein ligase activity of RNF144A:PRKDC:Ub:UBE2L3 [cytosol]

Orthologous Events
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