ALDOB tetramer cleaves Fru-1-P to GA and DHAP

Stable Identifier
Homo sapiens
Aldolase B (ALDOB) cleaves fructose-1-phosphate to form D-glyceraldehyde and dihydroxyacetone phosphate, D-fructose-1-phosphate <=> D-glyceraldehyde + dihydroxyacetone phosphate
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Cytosolic aldolase B (ALDOB) catalyzes the reaction of D-fructose 1-phosphate (Fru 1-P) to form dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde (GA) (Hers & Kusaka 1953; Schapira 1975). The active form of the enzyme is a tetramer (Dalby et al. 2001). Deficiencies in the enzyme are associated with hereditary fructose intolerance in vivo (e.g., Tolan 1995; Ali et al. 1998).
ALDOB is the same aldolase isoform that catalyzes the reversible cleavage of fructose-1,6-bisphosphate in glycolysis. This isoform, found in liver, kidney, and intestine, is approximately equally active with fructose 1 phosphate and fructose 1,6 bisphosphate as substrates at saturating concentrations, while the muscle and brain isoforms (ALDOA and ALDOC, respectively), have little activity with fructose-1-phosphate (Lebherz & Rutter 1969; Penhoet et el. 1969).

Literature References
PubMed ID Title Journal Year
8535439 Molecular basis of hereditary fructose intolerance: mutations and polymorphisms in the human aldolase B gene

Tolan, DR

Hum Mutat 1995
5353106 Isolation of fructose diphosphate aldolases A, B, and C

Kochman, M, Rutter, WJ, Penhoet, EE

Biochemistry 1969
11679716 The structure of human liver fructose-1,6-bisphosphate aldolase

Littlechild, JA, Tolan, DR, Dalby, AR

Acta Crystallogr D Biol Crystallogr 2001
13093749 [The metabolism of fructose-1-phosphate in the liver.]

Hers, HG, Kusaka, T

Biochim Biophys Acta 1953
5777313 Distribution of fructose diphosphate aldolase variants in biological systems

Rutter, WJ, Lebherz, HG

Biochemistry 1969
805723 Kinetic and immunological abnormalities of aldolase B in hereditary fructose intolerance

Schapira, F

Biochem. Soc. Trans. 1975
9610797 Hereditary fructose intolerance

Cox, TM, Ali, M, Rellos, P

J. Med. Genet. 1998
Catalyst Activity

fructose-1-phosphate aldolase activity of ALDOB tetramer [cytosol]

Orthologous Events
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