ALDOB tetramer cleaves Fru-1-P to GA and DHAP

Stable Identifier
Reaction [transition]
Homo sapiens
beta-D-fructose 1-phosphate => D-glyceraldehyde + dihydroxyacetone phosphate, Aldolase B (ALDOB) cleaves fructose-1-phosphate to form D-glyceraldehyde and dihydroxyacetone phosphate, D-fructose-1-phosphate <=> D-glyceraldehyde + dihydroxyacetone phosphate
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
Cytosolic aldolase B (ALDOB) catalyzes the reaction of D-fructose 1-phosphate (Fru 1-P) to form dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde (GA) (Hers & Kusaka 1953; Schapira 1975). The active form of the enzyme is a tetramer (Dalby et al. 2001). Deficiencies in the enzyme are associated with hereditary fructose intolerance in vivo (e.g., Tolan 1995; Ali et al. 1998).
ALDOB is the same aldolase isoform that catalyzes the reversible cleavage of fructose-1,6-bisphosphate in glycolysis. This isoform, found in liver, kidney, and intestine, is approximately equally active with fructose 1 phosphate and fructose 1,6 bisphosphate as substrates at saturating concentrations, while the muscle and brain isoforms (ALDOA and ALDOC, respectively), have little activity with fructose-1-phosphate (Lebherz & Rutter 1969; Penhoet et el. 1969).
Literature References
PubMed ID Title Journal Year
8535439 Molecular basis of hereditary fructose intolerance: mutations and polymorphisms in the human aldolase B gene

Tolan, DR

Hum Mutat 1995
5353106 Isolation of fructose diphosphate aldolases A, B, and C

Kochman, M, Rutter, WJ, Penhoet, EE

Biochemistry 1969
11679716 The structure of human liver fructose-1,6-bisphosphate aldolase

Littlechild, JA, Tolan, DR, Dalby, AR

Acta Crystallogr D Biol Crystallogr 2001
13093749 [The metabolism of fructose-1-phosphate in the liver.]

Hers, HG, Kusaka, T

Biochim Biophys Acta 1953
5777313 Distribution of fructose diphosphate aldolase variants in biological systems

Rutter, WJ, Lebherz, HG

Biochemistry 1969
805723 Kinetic and immunological abnormalities of aldolase B in hereditary fructose intolerance

Schapira, F

Biochem. Soc. Trans. 1975
9610797 Hereditary fructose intolerance

Cox, TM, Ali, M, Rellos, P

J. Med. Genet. 1998
Catalyst Activity

fructose-1-phosphate aldolase activity of ALDOB tetramer [cytosol]

Orthologous Events
Cross References
Cite Us!