Reactome | 30S pre-rRNA is nucleolytically processed at site 1 to yield 21S pre-rRNA

30S pre-rRNA is nucleolytically processed at site 1 to yield 21S pre-rRNA

Stable Identifier

R-HSA-6791221

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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30S pre-rRNA is nucleolytically processed at site 1 to yield 21S pre-rRNA

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An unknown endonuclease cleaves site 1 (also called site A1), removing the remainder of the 5' external transcribed spacer (5' ETS) from 30S pre-rRNA containing the 18S rRNA (Freed et al. 2012, Tomecki et al. 2015). Sixteen proteins of the small ribosomal subunit are required for processing sequences flanking the 18S rRNA in the 30S pre-rRNA (O'Donohue et al. 2010) presumably due to their assembly onto pre-rRNA as processing proceeds. Additionally, FCF1 (hUTP24, part of the SSU processome) is required for cleavage at site 1 (Tomecki et al. 2015) and NOL11 and CIRH1A (Cirhin, part of the UTPA complex) interact and are required for cleavage at site 1 and other sites (A', A0, and 2) (Freed et al. 2012). FTSJ3 and NIP7 interact and are required for processing at sites 1, 2, and A0 (Morello et al. 2011).

Literature References

PubMed ID	Title	Journal	Year
22916032	NOL11, implicated in the pathogenesis of North American Indian childhood cirrhosis, is required for pre-rRNA transcription and processing Freed, EF, Prieto, JL, McCann, KL, McStay, B, Baserga, SJ	PLoS Genet.	2012
20798176	The NIP7 protein is required for accurate pre-rRNA processing in human cells Morello, LG, Hesling, C, Coltri, PP, Castilho, BA, Rimokh, R, Zanchin, NI	Nucleic Acids Res.	2011
20819938	Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits O'Donohue, MF, Choesmel, V, Faubladier, M, Fichant, G, Gleizes, PE	J. Cell Biol.	2010
22195017	The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing Morello, LG, Coltri, PP, Quaresma, AJ, Simabuco, FM, Silva, TC, Singh, G, Nickerson, JA, Oliveira, CC, Moore, MJ, Zanchin, NI	PLoS ONE	2011
26237581	hUTP24 is essential for processing of the human ribosomal RNA precursor at site A1, but not at site A0 Tomecki, R, Labno, A, Drazkowska, K, Cysewski, D, Dziembowski, A	RNA Biol	2015