QTRT1:QTRTD1 exchanges guanine for queuine at guanosine-34 of tRNA(Asn),tRNA(Asp),tRNA(His),tRNA(Tyr) yielding queuosine-34 tRNA(Asn),tRNA(Asp),tRNA(His),tRNA(Tyr)

Stable Identifier
R-HSA-6782443
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol, mitochondrial outer membrane
ReviewStatus
5/5
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QTRT1:QTRTD1 exchanges guanine for queuine at guanosine-34 of tRNA(Asn),tRNA(Asp),tRNA(His),tRNA(Tyr) yielding queuosine-34 tRNA(Asn),tRNA(Asp),tRNA(His),tRNA(Tyr)
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The transglycosylase complex QTRT1:QTRTD1 exchanges guanine for queuine at nucleotide 34 of tRNA(Tyr) (Chen et al. 2010, Chen et al. 2011). The QTRT1 subunit is responsible for the transglycosylase activity. Eukaryotes are unable to synthesize queuine and must obtain it from dietary sources or symbiotic gut flora. As inferred from the mouse homologs, QTRT1:QTRTD1 associates with the outer mitochondrial membrane (Boland et al. 2009). The homologous enzyme in Escherichia coli is tgt.
Literature References
PubMed ID Title Journal Year
21131277 Evolution of eukaryal tRNA-guanine transglycosylase: insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases

Chen, YC, Brooks, AF, Goodenough-Lashua, DM, Kittendorf, JD, Showalter, HD, Garcia, GA

Nucleic Acids Res. 2011
20354154 Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure

Chen, YC, Kelly, VP, Stachura, SV, Garcia, GA

RNA 2010
19414587 Queuosine formation in eukaryotic tRNA occurs via a mitochondria-localized heteromeric transglycosylase

Boland, C, Hayes, P, Santa-Maria, I, Nishimura, S, Kelly, VP

J. Biol. Chem. 2009
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Output
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Event Information
Go Biological Process
tRNA modification (0006400)
Catalyst Activity

tRNA-guanosine(34) queuine transglycosylase activity of QTRT1:QTRTD1 [mitochondrial outer membrane]

Physical Entity
QTRT1:QTRTD1 [mitochondrial outer membrane] (Homo sapiens)
Activity
tRNA-guanosine(34) queuine transglycosylase activity (GO:0008479)
Authored
May, B  (2015-06-07)
Reviewed
Levinger, L  (2015-08-11)
Motorin, Y  (2015-08-25)
Jarrous, N  (2015-10-24)
Swairjo, MA  (2025-03-17)
May, B  (2025-06-02)
Created
May, B  (2015-06-08)
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