The Beclin-1 complex (ATG14:PIK3C3:PIK3R4:BECN1) associates with the endoplasmic reticulum (ER). The ATG14 component of the complex contains an ER-binding motif in its N-terminal domain, which appears to be essential for its function in autophagy and for the recruitment of the other Beclin-1 complex subunits to the site of phagophore formation (Matsunaga et al. 2010). N-terminal myristoylation of the PIK3R4 (p150) component may also anchor the Beclin-1 complex to the membrane (Panaretou et al. 1997, Wirth et al. 2013).