botB HC:LC binds SYT1 or 2 and GT1b on the target cell surface

Stable Identifier
R-HSA-5244397
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
extracellular region, plasma membrane
ReviewStatus
5/5
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botB HC:LC binds SYT1 or 2 and GT1b on the target cell surface
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The Botulinum toxin type B disulfide-bonded heavy chain - light chain dimer (botB HC:LC, encoded by the C. botulinum botB gene) (Swaminathan & Eswaramoorthy 2000) binds ganglioside GT1b and syntagmin 1 or 2 (SYT1 or 2) on the plasma membrane of a human target cell. In vivo, this process specifically targets synapses at neuromuscular junctions, where toxin association with ganglioside may position it to bind efficiently to SYT1 or 2 when those proteins are exposed at the cell surface by exocytosis (Dong et al. 2003). In vitro, botB HC:LC can bind gangliosides in addition to GT1b but with lower affinity (Kozaki et al. 1998). Only GT1b binding is annotated here.
Literature References
PubMed ID Title Journal Year
10932256 Structural analysis of the catalytic and binding sites of Clostridium botulinum neurotoxin B

Swaminathan, S, Eswaramoorthy, S

Nat Struct Biol 2000
14504267 Synaptotagmins I and II mediate entry of botulinum neurotoxin B into cells

Tepp, WH, Chapman, ER, Dong, M, Goodnough, MC, Johnson, EA, Richards, DA

J Cell Biol 2003
9712688 Ganglioside GT1b as a complementary receptor component for Clostridium botulinum neurotoxins

Mochida, S, Kamata, Y, Kozaki, S, Nishiki, T, Watarai, S

Microb. Pathog. 1998
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Output
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as an event of
Disease
Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Authored
Gopinathrao, G  (2006-06-15)
Krupa, S  (2006-06-15)
Reviewed
Ichtchenko, K  (2007-08-03)
Thirunavukkarasu, N  (2014-11-18)
Sharma, S  (2014-11-18)
Created
D'Eustachio, P  (2014-01-18)
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