TDG excises 5-carboxylcytosine

Stable Identifier
Reaction [transition]
Homo sapiens
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Thymine DNA glycosylase (TDG) excises 5-carboxylcytosine (5-caC) from DNA (Maiti and Drohat 2011, Hashimoto et al. 2012, Zhang et al. 2012, inferred from mouse in He et al. 2011) by flipping the base out of the helix and cleaving the N-glycosidic bond to leave an abasic site (apurinic/apyrimidinic site, AP site) (Hashimoto et al. 2012, Zhang et al. 2012). TDG interacts with the G opposite the excised base and remains bound to the abasic site (Maiti et al. 2008). Dissociation of TDG from DNA is the rate-limiting step of the reaction.

Literature References
PubMed ID Title Journal Year
21817016 Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA

Li, BZ, Tang, Q, Sun, Y, Jia, Y, Li, X, Chen, Z, Song, CX, Wang, Y, Li, Z, Liu, P, Zhang, K, Ding, J, Li, L, Dai, Q, Xu, GL, He, C, He, YF

Science 2011
18587051 Crystal structure of human thymine DNA glycosylase bound to DNA elucidates sequence-specific mismatch recognition

Morgan, MT, Pozharski, E, Maiti, A, Drohat, AC

Proc. Natl. Acad. Sci. U.S.A. 2008
22327402 Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA

Lu, J, Liang, H, Lu, X, Dai, Q, He, C, Xu, GL, Jiang, H, Zhang, L, Luo, C

Nat. Chem. Biol. 2012
21862836 Thymine DNA glycosylase can rapidly excise 5-formylcytosine and 5-carboxylcytosine: potential implications for active demethylation of CpG sites

Maiti, A, Drohat, AC

J. Biol. Chem. 2011
22962365 Excision of 5-hydroxymethyluracil and 5-carboxylcytosine by the thymine DNA glycosylase domain: its structural basis and implications for active DNA demethylation

Hong, S, Hashimoto, H, Zhang, X, Bhagwat, AS, Cheng, X

Nucleic Acids Res. 2012
Event Information
Catalyst Activity

DNA N-glycosylase activity of TDG [nucleoplasm]

Orthologous Events
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