Reactome | TDG excises 5-carboxylcytosine

TDG excises 5-carboxylcytosine

Stable Identifier

R-HSA-5221061

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Thymine DNA glycosylase (TDG) excises 5-carboxylcytosine (5-caC) from DNA (Maiti and Drohat 2011, Hashimoto et al. 2012, Zhang et al. 2012, inferred from mouse in He et al. 2011) by flipping the base out of the helix and cleaving the N-glycosidic bond to leave an abasic site (apurinic/apyrimidinic site, AP site) (Hashimoto et al. 2012, Zhang et al. 2012). TDG interacts with the G opposite the excised base and remains bound to the abasic site (Maiti et al. 2008). Dissociation of TDG from DNA is the rate-limiting step of the reaction.

Literature References

PubMed ID	Title	Journal	Year
21817016	Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA He, YF, Li, BZ, Li, Z, Liu, P, Wang, Y, Tang, Q, Ding, J, Jia, Y, Chen, Z, Li, L, Sun, Y, Li, X, Dai, Q, Song, CX, Zhang, K, He, C, Xu, GL	Science	2011
18587051	Crystal structure of human thymine DNA glycosylase bound to DNA elucidates sequence-specific mismatch recognition Maiti, A, Morgan, MT, Pozharski, E, Drohat, AC	Proc. Natl. Acad. Sci. U.S.A.	2008
22327402	Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA Zhang, L, Lu, X, Lu, J, Liang, H, Dai, Q, Xu, GL, Luo, C, Jiang, H, He, C	Nat. Chem. Biol.	2012
21862836	Thymine DNA glycosylase can rapidly excise 5-formylcytosine and 5-carboxylcytosine: potential implications for active demethylation of CpG sites Maiti, A, Drohat, AC	J. Biol. Chem.	2011
22962365	Excision of 5-hydroxymethyluracil and 5-carboxylcytosine by the thymine DNA glycosylase domain: its structural basis and implications for active DNA demethylation Hashimoto, H, Hong, S, Bhagwat, AS, Zhang, X, Cheng, X	Nucleic Acids Res.	2012