Crumbs forms a ternary complex with Pals1 and PATJ at tight junctions

Stable Identifier
R-HSA-420661
Type
Reaction [binding]
Species
Homo sapiens
Compartment
plasma membrane
ReviewStatus
5/5
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Crumbs forms a ternary complex with Pals1 and PATJ at tight junctions
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The CRB3:PALS1:PATJ complex is the second major cell polarity protein complex at tight junctions (TJs) (Shin et al., 2006). The integral membrane protein CRB3 localizes to the apical domain of epithelial cells and is concentrated at TJs. CRB3 directly associates with PALS1(MPP5) which interacts with PATJ, a protein consisting of 10 PDZ domains. The interaction with CRB3 might recruit the MPP5:PATJ complex to TJs (Lemmers et al., 2002; Roh et al., 2003). Although its precise functions of the individual components have not been established, the complex is required for TJ formation, in part through the stabilization of apical and lateral components of tight junctions (Michel et al., 2005; Shin et al., 2005).

Severe acute respiratory syndrome coronavirus type 1 )SARS-CoV-1) envelope (E) protein was found to interfere with host PDZ‐dependent interactions of MPP5 (PALS1) thus affecting TJ formation (Teoh KT et al. 2010). Similar data were reported for SARS-CoV-2 E ( Toto A et al. 2020; Javorsky A et al. 2021).

Literature References
PubMed ID Title Journal Year
16129888 PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells

Michel, D, Arsanto, JP, Massey-Harroche, D, Beclin, C, Wijnholds, J, Le Bivic, A

J Cell Sci 2005
11964389 hINADl/PATJ, a homolog of discs lost, interacts with crumbs and localizes to tight junctions in human epithelial cells

Lemmers, C, Médina, E, Delgrossi, MH, Michel, D, Arsanto, JP, Le Bivic, A

J Biol Chem 2002
Participants
Input
Output
Participates
as an event of
This event is regulated
Negatively by
Regulator
MMP5:E pentamer [endoplasmic reticulum-Golgi intermediate compartment membrane] (Homo sapiens)
Active Unit
Ub-3xPalmC-E pentamer [endoplasmic reticulum-Golgi intermediate compartment membrane]
Authored
Matthews, L  (2009-05-15)
Reviewed
Ebnet, K  (2009-08-27)
Messina, F  (2022-02-18)
Created
Matthews, L  (2009-05-12)
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