Phosphorylation of GORASP1, GOLGA2 and RAB1A by CDK1:CCNB

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R-HSA-2172183
Type
Reaction [transition]
Species
Homo sapiens
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GORASP1 (GRASP65) and GOLGA2 (GM130) form a complex on cis-Golgi membranes. RAB1A or RAB1B, small RAS GTP-ases, can also associate with this complex through interaction with GOLGA2 (Moyer et al. 2001, Weide et al. 2001). GOLGA2 provides a docking site for the USO1 (p115) homodimer (Nakamura et al. 1995, Seeman et al. 2000). RAB1 also participates in this interaction and facilitates it when in the GTP-bound state (Moyer et al. 2001). Binding of USO1 to GORASP1:GOLGA2:RAB1:GTP complex enables fusion of vesicles originating in the endoplasmic reticulum (ER) with cisternae of cis-Golgi.
In mitotic prophase, CDK1 (CDC2) in complex with either CCNB1 (cyclin B1) or CCNB2 (cyclin B2), as both CCNB1 and CCNB2 can localize to Golgi (Jackman et al. 1995, Draviam et al. 2001), phosphorylates GORASP1, GOLGA2 and RAB1 (Bailly et al. 1991, Lowe et al. 1998, Preisinger et al. 2005). Phosphorylation of GOLGA2 and RAB1 impairs their association with USO1, which inhibits thethering and subsequent fusion of ER-originating vesicles with cis-Golgi cisternae, resulting in cessation of ER to Golgi protein trafficking at the start of mitosis and increase in the number of Golgi trafficking vesicles at the expense of Golgi cisternae (Lowe et al. 1998, Seeman et al. 2000, Moyer et al. 2001, Diao et al. 2008).

Literature References
PubMed ID Title Journal Year
1902553 Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2

Bailly, E, McCaffrey, M, Touchot, N, Zahraoui, A, Goud, B, Bornens, M

Nature 1991
11285137 Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering

Moyer, BD, Allan, BB, Balch, WE

Traffic 2001
7737117 Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus

Jackman, M, Firth, M, Pines, J

EMBO J 1995
15678101 Plk1 docking to GRASP65 phosphorylated by Cdk1 suggests a mechanism for Golgi checkpoint signalling

Preisinger, C, Korner, R, Wind, M, Lehmann, WD, Kopajtich, R, Barr, FA

EMBO J 2005
9150144 The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner

Nakamura, N, Lowe, M, Levine, TP, Rabouille, C, Warren, G

Cell 1997
11238451 The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus

Draviam, VM, Orrechia, S, Lowe, M, Pardi, R, Pines, J

J Cell Biol 2001
9753325 Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis

Lowe, M, Rabouille, C, Nakamura, N, Watson, R, Jackman, M, Jämsä, E, Rahman, D, Pappin, DJC, Warren, G

Cell 1998
11306556 The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b

Weide, T, Bayer, M, Köster, M, Siebrasse, JP, Peters, R, Barnekow, A

EMBO Rep. 2001
10679020 The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo

Seemann, J, Jokitalo, EJ, Warren, G

Mol. Biol. Cell 2000
18167358 Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner

Diao, A, Frost, L, Morohashi, Y, Lowe, M

J. Biol. Chem. 2008
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Title
cyclin-dependent protein serine/threonine kinase activity of CCNB1,CCNB2:p-T161-CDK1 [cytosol]
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