Reactome | Cleavage of DNA by DFF40

Cleavage of DNA by DFF40

Stable Identifier

R-HSA-211247

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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The DFF40 cleaves DNA substrates to generate fragments possessing ends with 5’-phosphate and 3’-hydroxyl groups, and generates exclusively double strand breaks (primarily blunt ends). It has some sequence preferences on naked DNA substrates and prefers purine/pyrimidine blocks with rotational symmetry (Widlak et al., 2000). DFF is both a deoxyribonucleotide-specific and a double-strand-specific endonuclease (Hanus et al., 2008).

Literature References

PubMed ID	Title	Journal	Year
10713148	Cleavage preferences of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease) on naked DNA and chromatin substrates Li, P, Wang, X, Widlak, P, Garrard, WT	J Biol Chem	2000
18283539	The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme Hanus, J, Kalinowska-Herok, M, Widlak, P	Apoptosis	2008