The DFF40 cleaves DNA substrates to generate fragments possessing ends with 5’-phosphate and 3’-hydroxyl groups, and generates exclusively double strand breaks (primarily blunt ends). It has some sequence preferences on naked DNA substrates and prefers purine/pyrimidine blocks with rotational symmetry (Widlak et al., 2000). DFF is both a deoxyribonucleotide-specific and a double-strand-specific endonuclease (Hanus et al., 2008).
Li, P, Wang, X, Widlak, P, Garrard, WT
Hanus, J, Kalinowska-Herok, M, Widlak, P
deoxyribonuclease activity of DFF40 associated with chromatin [nucleoplasm]
© 2023 Reactome
