Cleavage of DNA by DFF40

Stable Identifier
R-HSA-211247
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The DFF40 cleaves DNA substrates to generate fragments possessing ends with 5’-phosphate and 3’-hydroxyl groups, and generates exclusively double strand breaks (primarily blunt ends). It has some sequence preferences on naked DNA substrates and prefers purine/pyrimidine blocks with rotational symmetry (Widlak et al., 2000). DFF is both a deoxyribonucleotide-specific and a double-strand-specific endonuclease (Hanus et al., 2008).

Literature References
PubMed ID Title Journal Year
18283539 The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme

Hanus, J, Kalinowska-Herok, M, Widlak, P

Apoptosis 2008
10713148 Cleavage preferences of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease) on naked DNA and chromatin substrates

Widlak, P, Li, P, Wang, X, Garrard, WT

J Biol Chem 2000
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
deoxyribonuclease activity of DFF40 associated with chromatin [nucleoplasm]
Physical Entity
Activity
This event is regulated
Orthologous Events
Authored
Reviewed
Created