Reactome | SEC31:SEC13 and v-SNARE recruitment

SEC31:SEC13 and v-SNARE recruitment

Stable Identifier

R-HSA-204008

Type

Reaction [binding]

Species

Homo sapiens

Compartment

endoplasmic reticulum membrane, cytosol

ReviewStatus

5/5

Locations in the PathwayBrowser

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Metabolism of proteins (Homo sapiens)

- Post-translational protein modification (Homo sapiens)
  - Asparagine N-linked glycosylation (Homo sapiens)
    - Transport to the Golgi and subsequent modification (Homo sapiens)
      - ER to Golgi Anterograde Transport (Homo sapiens)
        
        COPII-mediated vesicle transport (Homo sapiens)
        
        SEC31:SEC13 and v-SNARE recruitment (Homo sapiens)

General

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The outer coat proteins SEC13 and SEC31A are thought to exist in a cytosolic heterohexamer with SEC23IP and are recruited to the ER through interaction with pre-bound SEC23-SEC24 complexes (Stephens et al, 2000; Hammond and Glick, 2000; Stagg et al. 2006; Ong et al, 2010; reviewed in Szul and Sztul, 2011). Human SEC31B is close to 50% identical to human SEC31A (Tang et al. 2000, Stankewich et al. 2006). While SEC31A is ubiquitously and abundantly expressed, SEC31B is expressed at a high level in testis (Tang et al. 2000: Northern blot; Stankewich et al. 2006: Northern blot and dot-blot), thymus (Tang et al. 2000: Northern blot; Stankewich et al. 2006: dot-blot), cerebellum (Stankewich et al. 2006: dot-blot), lymph node (Stankewich et al. 2006: dot-blot), and pituitary gland (Stankewich et al. 2006: dot-blot) and at a low level in other tissues (Tang et al. 2000; Stankewich et al. 2006). SEC31B can form dimers with SEC31A (Stankewich et al. 2006) and it co-immunoprecipitates and co-localizes with at least two components of the COPII coat, SEC23 and SEC13 (Stankewich et al. 2006). The dynamic behavior of SEC31B in the cells is consistent with its participation with COPII-coated organelles (Stankewich et al. 2006). In a zebrafish model of retinal development, oligonucleotide-mediated gene knockdown of either sec31a or sec31b leads to loss of COPII function (Niu et al. 2014). In Arabidopsis, SEC31A and SEC31B were both shown to function as COPII components that are essential and interchangeable in pollen development (Liu et al. 2021). Based on experimental evidence described above, SEC31B is annotated as a candidate SEC31 component of the COPII coat. v-SNAREs such as the SEC22 proteins are also incorporated into the emerging vesicle through interactions with components of the COPII coat (Xu et al, 2000; Mancias and Goldberg, 2008; Gordon et al, 2010; reviewed in Szul and Sztul, 2011; Lord et al, 2013).

Literature References

PubMed ID	Title	Journal	Year
22013193	COPII and COPI traffic at the ER-Golgi interface Sztul, E, Szul, T	Physiology (Bethesda)	2011
10788476	Mammalian homologues of yeast sec31p. An ubiquitously expressed form is localized to endoplasmic reticulum (ER) exit sites and is essential for ER-Golgi transport Low, DY, Hong, W, Horstmann, H, Tang, BL, Wong, ET, Zhang, T	J Biol Chem	2000
10825291	COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites Paccaud, JP, Lin-Marq, N, Stephens, DJ, Pagano, A, Pepperkok, R	J Cell Sci	2000
16495487	Human Sec31B: a family of new mammalian orthologues of yeast Sec31p that associate with the COPII coat Morrow, JS, Stankewich, MC, Stabach, PR	J Cell Sci	2006
11035026	Subunit structure of a mammalian ER/Golgi SNARE complex Xu, D, Joglekar, AP, Williams, AL, Hay, JC	J. Biol. Chem.	2000
23378591	The highly conserved COPII coat complex sorts cargo from the endoplasmic reticulum and targets it to the golgi Ferro-Novick, S, Miller, EA, Lord, C	Cold Spring Harb Perspect Biol	2013
20679433	p125A exists as part of the mammalian Sec13/Sec31 COPII subcomplex to facilitate ER-Golgi transport Hong, W, Loo, LS, Ong, YS, Tang, BL	J. Cell Biol.	2010
16407955	Structure of the Sec13/31 COPII coat cage Balch, WE, LaPointe, P, Foss, TR, Carragher, B, Gürkan, C, Stagg, SM, Potter, CS, Fowler, DM	Nature	2006
20545907	A targeted siRNA screen to identify SNAREs required for constitutive secretion in mammalian cells Gordon, DE, Bond, LM, Sahlender, DA, Peden, AA	Traffic	2010
10982397	Dynamics of transitional endoplasmic reticulum sites in vertebrate cells Glick, BS, Hammond, AT	Mol Biol Cell	2000
18843296	Structural basis of cargo membrane protein discrimination by the human COPII coat machinery Goldberg, J, Mancias, JD	EMBO J.	2008