SEC31:SEC13 and v-SNARE recruitment

Stable Identifier
R-HSA-204008
Type
Reaction [binding]
Species
Homo sapiens
Compartment
endoplasmic reticulum membrane, cytosol
ReviewStatus
5/5
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
SEC31:SEC13 and v-SNARE recruitment
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
The outer coat proteins SEC13 and SEC31A are thought to exist in a cytosolic heterohexamer with SEC23IP and are recruited to the ER through interaction with pre-bound SEC23-SEC24 complexes (Stephens et al, 2000; Hammond and Glick, 2000; Stagg et al. 2006; Ong et al, 2010; reviewed in Szul and Sztul, 2011). Human SEC31B is close to 50% identical to human SEC31A (Tang et al. 2000, Stankewich et al. 2006). While SEC31A is ubiquitously and abundantly expressed, SEC31B is expressed at a high level in testis (Tang et al. 2000: Northern blot; Stankewich et al. 2006: Northern blot and dot-blot), thymus (Tang et al. 2000: Northern blot; Stankewich et al. 2006: dot-blot), cerebellum (Stankewich et al. 2006: dot-blot), lymph node (Stankewich et al. 2006: dot-blot), and pituitary gland (Stankewich et al. 2006: dot-blot) and at a low level in other tissues (Tang et al. 2000; Stankewich et al. 2006). SEC31B can form dimers with SEC31A (Stankewich et al. 2006) and it co-immunoprecipitates and co-localizes with at least two components of the COPII coat, SEC23 and SEC13 (Stankewich et al. 2006). The dynamic behavior of SEC31B in the cells is consistent with its participation with COPII-coated organelles (Stankewich et al. 2006). In a zebrafish model of retinal development, oligonucleotide-mediated gene knockdown of either sec31a or sec31b leads to loss of COPII function (Niu et al. 2014). In Arabidopsis, SEC31A and SEC31B were both shown to function as COPII components that are essential and interchangeable in pollen development (Liu et al. 2021). Based on experimental evidence described above, SEC31B is annotated as a candidate SEC31 component of the COPII coat. v-SNAREs such as the SEC22 proteins are also incorporated into the emerging vesicle through interactions with components of the COPII coat (Xu et al, 2000; Mancias and Goldberg, 2008; Gordon et al, 2010; reviewed in Szul and Sztul, 2011; Lord et al, 2013).
Literature References
PubMed ID Title Journal Year
22013193 COPII and COPI traffic at the ER-Golgi interface

Szul, T, Sztul, E

Physiology (Bethesda) 2011
10788476 Mammalian homologues of yeast sec31p. An ubiquitously expressed form is localized to endoplasmic reticulum (ER) exit sites and is essential for ER-Golgi transport

Tang, BL, Zhang, T, Low, DY, Wong, ET, Horstmann, H, Hong, W

J Biol Chem 2000
10825291 COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites

Stephens, DJ, Lin-Marq, N, Pagano, A, Pepperkok, R, Paccaud, JP

J Cell Sci 2000
16495487 Human Sec31B: a family of new mammalian orthologues of yeast Sec31p that associate with the COPII coat

Stankewich, MC, Stabach, PR, Morrow, JS

J Cell Sci 2006
11035026 Subunit structure of a mammalian ER/Golgi SNARE complex

Xu, D, Joglekar, AP, Williams, AL, Hay, JC

J. Biol. Chem. 2000
23378591 The highly conserved COPII coat complex sorts cargo from the endoplasmic reticulum and targets it to the golgi

Lord, C, Ferro-Novick, S, Miller, EA

Cold Spring Harb Perspect Biol 2013
20679433 p125A exists as part of the mammalian Sec13/Sec31 COPII subcomplex to facilitate ER-Golgi transport

Ong, YS, Tang, BL, Loo, LS, Hong, W

J. Cell Biol. 2010
16407955 Structure of the Sec13/31 COPII coat cage

Stagg, SM, Gürkan, C, Fowler, DM, LaPointe, P, Foss, TR, Potter, CS, Carragher, B, Balch, WE

Nature 2006
20545907 A targeted siRNA screen to identify SNAREs required for constitutive secretion in mammalian cells

Gordon, DE, Bond, LM, Sahlender, DA, Peden, AA

Traffic 2010
10982397 Dynamics of transitional endoplasmic reticulum sites in vertebrate cells

Hammond, AT, Glick, BS

Mol Biol Cell 2000
18843296 Structural basis of cargo membrane protein discrimination by the human COPII coat machinery

Mancias, JD, Goldberg, J

EMBO J. 2008
Participants
Input
Output
Participates
as an event of
Authored
Gillespie, ME  (2007-07-14)
Reviewed
Gagneux, P  (2010-11-18)
Matthews, L  (2024-04-10)
Created
Gillespie, ME  (2007-11-22)
Cite Us!