botB LC cleaves target cell VAMP2

Stable Identifier
R-HSA-194796
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
cytosol, synaptic vesicle membrane
ReviewStatus
5/5
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botB LC cleaves target cell VAMP2
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Botulinum toxin type B light chain (botB LC), in the cytosol of a target cell, catalyzes the removal of an aminoterminal peptide from vesicle-associated membrane protein 2 (VAMP2). botB LC is a zinc metalloprotease (Foran et al. 1994; Schiavo et al. 1992). VAMP2 is associated with the cytosolic face of the target cell synaptic vesicle and is required for vesicle docking and exocytosis. Its cleavage by botulinum toxin blocks synaptic vesicle fusion with the plasma membrane and neurotransmitter release and in vivo leads to a long lasting flaccid paralysis (Sudhof et al, 1993; Sudhof 2004).
Literature References
PubMed ID Title Journal Year
1331807 Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin

Schiavo, G, Benfenati, F, Poulain, B, Rossetto, O, Polverino de Laureto, P, DasGupta, BR, Montecucco, C

Nature 1992
8402889 Membrane fusion machinery: insights from synaptic proteins

Südhof, TC, De Camilli, P, Niemann, H, Jahn, R

Cell 1993
7803399 Differences in the protease activities of tetanus and botulinum B toxins revealed by the cleavage of vesicle-associated membrane protein and various sized fragments

Foran, P, Shone, CC, Dolly, JO

Biochemistry 1994
15217342 The synaptic vesicle cycle

Südhof, TC

Annu Rev Neurosci 2004
Participants
Input
Output
Participates
as an event of
Catalyst Activity

metalloendopeptidase activity of botB LC:Zn2+ [cytosol]

Physical Entity
botB LC:Zn2+ [cytosol] (Clostridium botulinum)
Activity
metalloendopeptidase activity (GO:0004222)
Disease
Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Cross References
Mondo
Authored
Krupa, S  (2006-06-15)
Gopinathrao, G  (2006-06-15)
Reviewed
Ichtchenko, K  (2007-08-03)
Thirunavukkarasu, N  (2014-11-18)
Sharma, S  (2014-11-18)
Created
Gopinathrao, G  (2007-03-27)
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